Crystal violet

Dimethyl sulfoxide (DMSO), Crystal violet and trypan blue dye were obtained from Sigma (St. Louis, MO, USA). Crystal violet stain was prepared as 1% using 0.5% (w/v) Crystal violet and 50% methanol that were adjusted to volume using distilled water and subsequently filtered through a Whatman No.1 filter paper. The studied bacterial strain was Escherichia coli, ATCC 25922, obtained from the American type culture collection (ATCC).

MRC-5 (normal human Lung fibroblast cells) were obtained from VACSERA Tissue Culture Unit. PC-3 cells (human prostate carcinoma cell line) were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA). The normal human lung fibroblasts (MRC-5) cells were propagated in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% heat-inactivated Fetal bovine serum, 1% L-glutamine, HEPES buffer, and 50µg/mL gentamycin. The human prostate carcinoma cell line PC3 cells were grown on RPMI-1640 medium supplemented with 10% inactivated fetal calf serum and 50 µg/mL gentamycin. All cells were maintained at 37 °C in a humidified atmosphere with 5% CO₂ and were subcultured two times a week. Chemicals used: Dimethyl sulfoxide (DMSO), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide), crystal violet, and trypan blue dye were purchased from Sigma (St. Louis, MO, USA). Fetal bovine serum, DMEM, RPMI-1640, HEPES buffer solution, L-glutamine, gentamycin, and 0.25% trypsin-EDTA were purchased from Lonza. crystal violet stain (1%): composed of 0.5% (w/v) crystal violet and 50% methanol then made up to volume with deionized water and filtered through a Whatman No.1 filter paper.