Samples of yellow ligamentum flavum collected from patients in the study group and participants in the control group, minced using a scalpel, were transferred to a new Eppendorf tube and weighed. The prepared samples were incubated with a solution of 4 M guanidine hydrochloride (Sigma Aldrich St. Louis, MO, USA), 1 M sodium acetate (Sigma Aldrich St. Louis, MO, USA), Triton 2% (Sigma Aldrich St. Louis, MO, USA). and protease inhibitor cocktail (Sigma Aldrich St. Louis, MO, USA) for 12 hours at 4°C on a laboratory rocker. Next, the samples were centrifuged, and the obtained supernatant was stored at −20°C until the next stage began.
Triton 2
Manufactured by Merck Group
Sourced in United States
Triton 2% is a surfactant product used in various laboratory applications. It functions as a detergent and emulsifier to aid in the solubilization and dispersion of materials.
Automatically generated - may contain errors
2 protocols using triton 2
1
Ligamentum Flavum Protein Extraction
2
Protein Extraction from IVD Samples
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Using a scalpel, IVD samples were cut into pieces approximately 1 mm in size, placed in a new test tube, and weighed. The weight of the sample was determined by subtracting the weight of the test tube from the weight of the sample in the test tube. The samples were incubated with a solution of 4M guanidine hydrochloride (Sigma Aldrich St. Louis, MO, USA, catalog number G3272), 1M sodium acetate (Sigma Aldrich St. Louis, MO, USA, catalog number S2889-250G), Triton 2% (Sigma Aldrich St. Louis, MO, USA, catalog number X100), and protease inhibitor cocktail (Sigma Aldrich St. Louis, MO, USA, catalog number P8340) for 12 h at 4°C on a laboratory cradle. The samples were then centrifuged (3000 rpm, 10 min) to obtain the supernatant, which was stored at −20°C.
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