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Sybr green fluorescence

Manufactured by Nippon Gene
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Sourced in Japan

SYBR Green is a fluorescent dye used in molecular biology for the detection and quantification of DNA. It binds to double-stranded DNA, resulting in a fluorescent signal that can be measured and used to determine the amount of DNA present in a sample.

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Lab products found in correlation

Lightcycler 480 detector, by Roche (2 mentions) Easyspin plus tissue cell rna extraction kit, by Aidlab (2 mentions) Hiscript q rt supermix kit, by Vazyme (2 mentions)

2 protocols using sybr green fluorescence

1

Gene Expression Analysis in Cells

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Total RNA was isolated using the EASYspin Plus tissue/cell RNA extraction kit (Aidlab, China) according to the manufacturer's protocol. One microgram of total RNA was reverse transcribed using the HiScript Q RT SuperMix kit (Vazyme, China). Quantitative PCR was then performed using TaqMan polymerase with SYBR Green fluorescence (Nippon Gene, Japan) on a LightCycler 480 Detector (Roche, Germany). Target gene threshold cycles (Ct values) were normalized to GAPDH as an endogenous control. Primer sequences: GAPDH forward, 5'-GAAAGCCTGCCGGTGACTAA-3' and reverse, 5'-AGGAAAAGCATCACCCG GAG-3'; Bax forward, 5'-CCAGAGGCGGGGTTTCAT-3' and reverse, 5'-CATCCTCTGCAGCTCCATGT-3'; Bcl2 forward, 5'-GAACTGGGGGAGGATTGTGG-3' and reverse, 5'-CATCCCAGCCTCCGTTATCC-3'); FASTKD2 forward, 5'-TCCTGAATCCCTAAACATGAAAA-3' and reverse, 5'-GCCATAACTTCCACGAACTG-3'; Caspase3 forward, 5'-GAAAGCCGAAACTCTTCATCAT-3' and reverse, 5'-ATGCCATATCATCGTCAGTTCC-3'.
Zhang Y.F., Yuan L.L., Wang Z.C., Zhuang W.B., Zhang W.J., Liu H.T., Li M, & Fan L.H. (2024). DHEA down-regulates mitochondrial dynamics and promotes apoptosis of lung adenocarcinoma cells through FASTKD2. Journal of Cancer, 15(8), 2110-2122.
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2

Total RNA Extraction and qPCR Analysis

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Total RNA was isolated using the EASYspin Plus tissue/cell RNA extraction kit (Aidlab, China) according to the manufacturer's protocol. One microgram of total RNA was reverse transcribed using the HiScript Q RT SuperMix kit (Vazyme, China). Quantitative PCR was then performed using TaqMan polymerase with SYBR Green fluorescence (Nippon Gene, Japan) on a LightCycler 480 Detector (Roche, Germany). Target gene threshold cycles (Ct values) were normalized to GAPDH as an endogenous control. The primer sequences are listed in Table S2.
Luo J., Xia Y., Yin Y., Luo J., Liu M., Zhang H., Zhang C., Zhao Y., Yang L, & Kong L. (2019). ATF4 destabilizes RET through nonclassical GRP78 inhibition to enhance chemosensitivity to bortezomib in human osteosarcoma. Theranostics, 9(21), 6334-6353.
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