Protein chemistry reagents and buffers

All organic solvents were analytical grade reagents (AR) procured from Merck Chemical Inc. (Darmstadt, Germany). MTT (3-[4,5-dimethylthiazol-2yl]-2.5-diphenylterazolium bromide) was obtained from Sigma Aldrich company (St Louis, MO, USA). Fetal bovine serum (FBS), Dulbecco’s Modified Eagle Medium (DMEM)/high glucose, L-glutamine and penicillin/streptomycin were purchased from Gibco Inc. (NY, USA). Total RNA extraction, cDNA synthesis, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) kits, reagents and PCR oligo primers were ordered and purchased from Qiagen (Hilden, Germany). Corning^® 96-well clear flat-bottom polystyrene TC-treated Microplates, Corning^® 75 cm² cell culture flasks with vent cap, Falcon^® (15 and 50 mL) polystyrene centrifuge tubes and sterile individually-wrapped Stripette™ serological polystyrene pipettes were purchased from Corning^® USA. All protein chemistry reagents and buffers were obtained from Bio-Rad Laboratories GmbH (Munich, Germany).

MTT (3-[4,5-dimethylthiazol-2yl]-2.5-diphenylterazolium bromide) was purchased from Sigma Aldrich (St Louis, MO, USA). DMEM, FBS, l-glutamine and penicillin/streptomycin were obtained from Hyclone Laboratories (Logan, UT, USA). Primers for 16s sequencing, PCR and RT-qPCR were ordered from Macrogen (Seoul, South Korea). Kits for cDNA reverse transcription and SYBR Green master mix were purchased from Qiagen (Hilden, Germany). All protein chemistry reagents and buffers were obtained from Bio-Rad Laboratories GmbH (Munich, Germany).