Calcein acetoxymethyl ester calcein am

Manufactured by Beyotime

Sourced in China

Calcein acetoxymethyl ester (Calcein-AM) is a fluorescent dye commonly used in cell biology and biochemistry. It is a cell-permeant compound that can be used to measure cell viability and proliferation. Calcein-AM is non-fluorescent until it enters a live cell and is hydrolyzed by intracellular esterases, producing a green fluorescent signal.

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Lab products found in correlation

Propidium iodide, by Beyotime (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Abcam (1 mentions) Penicillin streptomycin solution p s, by Thermo Fisher Scientific (1 mentions) Anti cd44 antibody, by BioLegend (1 mentions) Oleic acid, by Sinopharm (1 mentions) Cck 8, by Dojindo Laboratories (1 mentions) Crystal violet staining solution, by Keygen Biotech (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Paclitaxel, by Selleck Chemicals (1 mentions) Gapdh, by Beyotime (1 mentions) Cell counting kit 8 cck 8, by Keygen Biotech (1 mentions) Annexin 5 fitc kit, by Beyotime (1 mentions) Matrix gel, by Beyotime (1 mentions) Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions) Testosterone, by Solarbio (1 mentions) Dcfh da, by Beyotime (1 mentions) Trypsase, by Beyotime (1 mentions)

Spelling variants of this product

Calcein AM (83 citations) Calcein (6 citations) Calcein-acetoxymethyl ester (5 citations)

5 protocols using calcein acetoxymethyl ester calcein am

Synthesis and Functionalization of Multifunctional Nanoparticles

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For material synthesis, 1‐octadecylamine, oleic acid, ethanol, Ca(NO₃)₂, Na₃PO₄, (3‐aminopropyl) triethoxysilane (APTES), ammonia, hydrochloric acid (HCl), sodium hydroxide (NaOH) were purchased from Sinopharm Chemical Reagent Co., Ltd. Dox. N‐Hydroxysulfosuccinimide sodium salt (Sulfo‐NHS) was purchased from Shanghai Macklin Biochemical Co., Ltd. Phosphate buffered saline (PBS), EDC, and Dox were purchased from Sigma‐Aldrich Chemical Reagent Co., Ltd. Anti‐CD44 antibody was purchased from BioLegend (America)
For cell experiments, minimum Essential Medium (MEM), α−minimum essential medium (α−MEM), fetal bovine serum (FBS), and Penicillin–Streptomycin solution (P/S) were purchased from Gibco (America). Cell Plasma Membrane Staining Kit with DiI (Red Fluorescence), Hoechst33342, propidium iodide (PI), and calcein acetoxymethyl ester (Calcein‐AM) were purchased from Beyotime Biotechnology Co., Ltd (China). DAPI was purchased from Abcam. CCK‐8 was purchased from Dojindo (Japan). Crystal violet staining solution and Annexin V‐kFluor488/PI staining assay were purchased from KeyGEN BioTECH (China).

Xia H., Hao M., Li K., Chen X., Yu L., Qiu J., Zhang H., Li H., Sang Y, & Liu H. (2023). CD44 and HAP‐Conjugated hADSCs as Living Materials for Targeted Tumor Therapy and Bone Regeneration. Advanced Science, 10(20), 2206393.

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Alantolactone and Brevilin A Cytotoxicity Evaluation

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Alantolactone (ALT) (>98% purity) was obtained from Tauto-Biotech (Shanghai, China) while Brevilin A (Brv-A) (purity >98%) and Paclitaxel (PTX) (purity >99%) was obtained from Selleck (China). DMEM (Dulbecco’s modified Eagle’s Medium), trypsin without or with EDTA, Streptomycin-Penicillin (10,000 U/ml), and fetal bovine serum (FBS) were all purchased from Gibco (Thermo Fisher Scientific, Inc.). Cell counting kit-8 (CCK-8) was purchased from KeyGen Biotech (Nanjing, China) and Protease-inhibitor cocktail from Sigma-Aldrich (St. Louis, MO). Dimethyl Sulfoxide (DMSO), Phenyl methyl sulfonyl fluoride (PMSF), Propidium Iodide (PI), crystal violet stain, Calcein acetoxymethyl ester (Calcein-AM), and annexin V-FITC kit for apoptosis detection were obtained from Beyotime Biotechnology (China). The primary antibodies for cleaved caspase-3, cleaved caspase-9 and cleaved PARP as well as p-STAT3, and STAT3 (Tyr 705) were obtained from Cell-Signaling Technology (Beverly, MA). FUT4 from Proteintech (Wuhan, China) while P-GP was obtained from abcam (Cambridge, MA). GAPDH was procured from Beyotime Biotechnology (China), while the HRP (Horseradish Peroxidase)-conjugated secondary type of antibodies (anti-mouse and anti-rabbit) were obtained from Sigma-Aldrich (St. Louis, MO).

Ding Y., Zhen Z., Nisar M.A., Ali F., Din R.U., Khan M., Mughal T.A., Alam G., Liu L, & Saleem M.Z. (2022). Sesquiterpene Lactones Attenuate Paclitaxel Resistance Via Inhibiting MALAT1/STAT3/ FUT4 Axis and P-Glycoprotein Transporters in Lung Cancer Cells. Frontiers in Pharmacology, 13, 795613.

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Biomimetic Tissue Engineering Scaffold

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Hyaluronic acid (HA), methacrylic anhydride, 2-hydroxy-4’-(2-hydroxyethoxy)-2-methylpropiophenone, poly (vinyl alcohol) (PVA), and Minoxidil were all purchased from Aladdin Bio-Chem Technology Co., Ltd (Shanghai, China). Poly (3-hydroxybutyric acid-co-3-hydroxyvaleric acid-co-3-hydroxyhexanoic acid trimer) (PHBVHHx or PBVHx), a novel trimer of PHA containing 90 mol% of 3-hydroxybutyrate, 3 mol% of 3-hydroxyvalerate and 7 mol% of 3-hydroxyhexanoate purchased from Bluepha Co., Ltd (Beijing, China). Testosterone was purchased from Solarbio Science & Technology Co., Ltd. (Shanghai, China). Cell Counting Kit-8 (CCK-8) was obtained from Dojindo Laboratories (Kumanoto, Japan). Calcein acetoxymethyl ester (Calcein-AM) and 3,8-diamino-5-[3-(diethylmethylammonio) propyl]-6-phenylphenanthridinium diiodide (PI), crystal violet staining solution, matrix-Gel™ (growth factor reduced) were obtained from Beyotime Biotechnology (Shanghai, China). VEGF and VEGFA ELISA Kit were purchased from ABclonal Technology Co., Ltd.

Ding Y.W., Li Y., Zhang Z.W., Dao J.W, & Wei D.X. (2024). Hydrogel forming microneedles loaded with VEGF and Ritlecitinib/polyhydroxyalkanoates nanoparticles for mini-invasive androgenetic alopecia treatment. Bioactive Materials, 38, 95-108.

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Osteoblast Cell Culture Protocol

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Polycaprolactone (PCL, Mw = 80000 Da) and penicillin-streptomycin (P&S) were purchased from Shanghai Yuanye Biochemical. Co., Ltd., Shanghai, China, Polyethylene glycol (PEG, Mw = 2000 Da) was obtained from Suzhou Sinopharm Co., Ltd., Suzhou, China, Stearic acid (SA, Analytical pure) and sodium dodecyl sulfonate (SDS) (purity ≥ 95%) were purchased from Aladdin Co., Ltd., Hongkong, China, Simvastatin (SIM, cell culture grade) was provided by Merrill Co., Ltd., New York, NY, USA, Tetrahydrofuran (THF) and anhydrous ethanol (AE) (analytically pure) were purchased from Jiangsu Qiangsheng Functional Chemistry Co., Ltd., Suzhou, China, N-butanol (Analytical Reagent) was purchased from Wuxi Zhanwang Chemical Reagent Co., Ltd., Wuxi, China, Phosphate buffer saline (PBS, PH 7.4) was purchased from Corning Co., Ltd., Glendale, CA, USA, Minimum essential medium-Alp (α-MEM) was purchased from Shanghai BasalMedia Technologies Co., Ltd., Shanghai, China, Fetal bovine serum (FBS) was purchased from Thermo Fisher Scientific, Waltham, MA, USA. Almar blue was purchased from Yeasen Biotechnology (Shanghai) Co., Ltd., Shanghai, China, Calcein acetoxy methyl ester (Calcein-AM) was purchased from Shanghai Beyotime Institute of Biotechnology, Shanghai, China. Mouse pre-osteoblasts (MC3T3-E1) were provided by Soochow University.

Pan L., Yang J, & Xu L. (2022). Preparation and Characterization of Simvastatin-Loaded PCL/PEG Nanofiber Membranes for Drug Sustained Release. Molecules, 27(21), 7158.

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Cytotoxicity Evaluation of Chlorinated Phenylalanine

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Manganese chloride (MnCl₂), tetramethylammonium hydroxide (TMAH), methylene blue (MB), H₂O₂, GSH, dimethyl sulfoxide (DMSO), 2-Chloro-Phe-OH [H-Phe(2-Cl)-OH 98%] were purchased from Shanghai Aladdin Bio-Chem Technology Co., Ltd. Methanol (CH₃OH, HPLC) and acetonitrile (CH₃CN, HPLC) were purchased from Fisher Chemical. RPMI-1640 medium was purchased from Wolcavi Biotechnology Co., Ltd (Beijing, China). Phosphate buffer and penicillin solution were purchased from Beijing Leagene Biotech Co., Ltd. Trypsase, DCFH-DA, Calcein acetoxymethyl ester (Calcein-AM) and propidium iodide (PI) were purchased from Beyotime Biotechnology (Shanghai, China).

Liu J., Wen C., Hu M., Long J., Zhang J., Li M, & Lin X.C. (2023). Metabolomics analysis of MnO2 nanosheets CDT for breast cancer cells and mechanism of cytotoxic action. RSC Advances, 13(38), 26630-26639.

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