Mig cxcl9 mouse elisa kit

To measure the concentration of CXCL9 in the skin, lesional and healthy skin were collected from wild-type and Ifnlr1^−/− mice at 6 dpi. Samples were weighed and then homogenized using beads and resuspended in 1 mL of DMEM. CXCL9 was measured from homogenized skin supernatants using the MIG/CXCL9 Mouse ELISA Kit (ThermoFisher, #EMCXCL9) using optical density read at 450 nm and fit against the standard curve according to manufacturer protocol.

The levels of tumor necrosis factor α (TNF-α), interferon-gamma (IFN-γ), CXCL10, and CXCL9 in the co-culture system were detected according to the instructions of Human TNF-α ELISA Kit (D711045, Shanghai Sangon Biological Engineering Technology & Services Co., Ltd., Shanghai, China), Human IFN-γ ELISA Kit (D711044, Sangon), Human CXCL10/IP-10 ELISA Kit (PC208, Beyotime), and MIG/CXCL9 Human ELISA Kit (EHCXCL9, Thermo Fisher).
The levels of TNF-α, IFN-γ, CXCL10, and CXCL9 in mouse xenograft tumors were detected according to the instructions of Mouse TNF-α ELISA Kit (PT512, Beyotime), Mouse IFN-γ ELISA Kit (PI508, Beyotime), Mouse CXCL10/IP-10 ELISA Kit (PC206, Beyotime), and MIG/CXCL9 Mouse ELISA Kit (EMCXCL9, Thermo Fisher).