A five-neck jacketed beaker (50 mL, Ace Glass Inc.), temperature controlled at 20 °C (recirculating bath, Julabo, F32-ME) and equipped with an overhead stirrer (J-Kem Scientific, OHS-1M) at 300 rpm was employed to evaluate the CCSA. The PAT probes of the CCSA (Incentive™ Vision IR/UV probe, ITA-Instruments Ltd) and the Raman spectrometer (Kaiser Optical Systems) were inserted into a jacketed beaker (Figure 1 ). The signals of the CCSA at 280, 340, 600, and 860 nm were recorded in 0.5 s intervals over 5 min utilizing the Incentive Vision Monitoring software (v. 1.1). The Raman spectra were obtained employing a R m nRxn2™ multi-channel spectrometer equipped with an immersion probe and a 785 nm laser using automatic cosmic ray filter and intensity correction (15 s exposure time, averaging 3 scans per measurement). The spectra were recorded in 1 min intervals and averaged over 5 min using the iC Raman software (v. 4.1.917). To prevent external light interference, the entire jacketed beaker was covered with aluminum foil (not shown in Figure 1 ).
F32 me
Manufactured by Julabo
Sourced in Germany
The Julabo F32-ME is a laboratory equipment designed for temperature control applications. It is a recirculating chiller that provides precise temperature regulation within a specified temperature range. The core function of the F32-ME is to circulate a temperature-controlled fluid through an external application.
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Lab products found in correlation
2 protocols using f32 me
1
Multimodal Characterization of CCSA
2
Viscosity Analysis of Chyme Samples
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The dynamic viscosity was determined in the chyme samples obtained from the in vitro digestion of model breads. The rheometer (Haake MARS 60, Thermo Fisher Scientific, Karlsruhe, Germany) was equipped with a circulating thermostat (F32-ME, Julabo GmbH, Seelbach, Germany) and a cone/plate measurement system (diameter = 50 mm, 1° cone angle, measurement gap: 0.052 mm, sample volume: 1 mL). The sample chamber was conditioned to 37 °C by Peltier temperature control modules to simulate human body temperature.
After loading the sample, the measuring system moved to the trim position (measurement gap plus 0.01 mm) and then to the measuring position. The sample was tempered to 37 °C for 120 s at a shear rate ɣ∴ of 0.000 1/s. Measurements were performed using CR (controlled rate) rotation and for the shear rate ramp the parameters ɣ∴ = 0.001 1/s (Ω = 0.0001677 1/min) to ɣ∴ = 100.0 1/s (Ω = 16.77 1/min) were applied with logarithmic data acquisition and continuous display for 100 s. Evaluation of the dynamic viscosity η was carried out using the software Haake RheoWin (version 4.87.0010, Thermo Fisher Scientific, Karlsruhe, Germany).
After loading the sample, the measuring system moved to the trim position (measurement gap plus 0.01 mm) and then to the measuring position. The sample was tempered to 37 °C for 120 s at a shear rate ɣ∴ of 0.000 1/s. Measurements were performed using CR (controlled rate) rotation and for the shear rate ramp the parameters ɣ∴ = 0.001 1/s (Ω = 0.0001677 1/min) to ɣ∴ = 100.0 1/s (Ω = 16.77 1/min) were applied with logarithmic data acquisition and continuous display for 100 s. Evaluation of the dynamic viscosity η was carried out using the software Haake RheoWin (version 4.87.0010, Thermo Fisher Scientific, Karlsruhe, Germany).
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