Anti vamp1

Bungarus caeruleus venom was supplied by Latoxan (France) and the lyophilized samples were kept at –20°C and reconstituted in phosphate-buffered saline solution (PBS) prior to use. The following primary antibodies were employed: anti-VAMP1 (1:200) [54 (link)], anti-Neurofilament (Abcam, cat. Ab 4680, 1:800), mAb-A06 (MT1) provided by Prof. M. Solimena [55 (link)], α-bungarotoxin Alexa555-conjugated (α-BTX555) (cat. B35451, 1:200) and secondary antibodies Alexa-conjugated (1:200) were from Life Technologies. Agomelatine (cat. A1362), Ramelteon (cat. SML2262), and melatonin (cat. M5250) were purchased from Sigma-Aldrich. Unless otherwise stated, all other reagents were from Sigma.

The following primary antibodies were employed: anti-VAMP1 (1:200, generated as described in [23 (link)]), anti-CXCR4 (Abcam, cat. Ab 124824, 1:400, Cambridge, UK), and anti-Neurofilament (Abcam, cat. Ab 4680, 1:800). The α-bungarotoxin (α-BTx) (cat. B35451, 1:200) and Alexa-conjugated secondary antibodies (1:200) were from Life Technologies. Unless otherwise stated, all other reagents were from Sigma. NUCC-390 was synthesized as previously described in [20 (link)].
The M. nigrocinctus venom was a pool of more than 50 specimens collected in the central and Pacific regions of Costa Rica and kept at the Serpentarium of Instituto Clodomiro Picado (University of Costa Rica). Once obtained, the venom was freeze-dried and stored at −20 °C.