Log In Sign up
The largest database of trusted experimental protocols

Antibodies for α sma

Manufactured by Abcam
Visit Supplier
Sourced in United Kingdom

Antibodies for α-SMA are used to detect and quantify the presence of alpha-smooth muscle actin (α-SMA) in biological samples. α-SMA is a cytoskeletal protein found in vascular smooth muscle cells and is commonly used as a marker for these cells.

Automatically generated - may contain errors

Lab products found in correlation

Recombinant human tgf β1 protein, by R&D Systems (1 mentions) 2 2 azobis 2 methylpropionamide dihydrochloride, by Yuanye Bio-Technology (1 mentions) Fluorescence tagged secondary antibodies, by Thermo Fisher Scientific (1 mentions) Phospho sapk jnk, by Cell Signaling Technology (1 mentions) Trypsin edta solution, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin cocktail, by Thermo Fisher Scientific (1 mentions) Cd31 antibody, by BD (1 mentions) Polyvinylidene fluoride membrane, by Bio-Rad (1 mentions) Propidium iodide, by Thermo Fisher Scientific (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Bromodeoxyuridine (brdu), by Merck Group (1 mentions) Mg132, by Merck Group (1 mentions) Cyclin d1, by Cell Signaling Technology (1 mentions) Chip assay kit, by Cell Signaling Technology (1 mentions) Cell lysis buffer 9803, by Cell Signaling Technology (1 mentions) Protein a g agarose gel, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

α-SMA (863 citations)

2 protocols using antibodies for α sma

1

Costunolide-Based Fibrosis Inhibition

Check if the same lab product or an alternative is used in the 5 most similar protocols
Costunolide (≥98%) was obtained from Nanjing Plant Origin Bio-Technology Co., Ltd. (Nanjing, China). Methacrylic acid copolymer Type A was obtained from Rohm (Germany). Tetraethyl orthosilicate (TEOS) and hexadecyl trimethyl ammonium bromide (CTAB) were purchased from Aladdin (Shanghai, China). 2,2′-Azobis (2-methylpropionamide) dihydrochloride (AIBA, ≥99%) was purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). L-lysine was purchased from Rhawn Reagent (Shanghai, China). Recombinant human TGF-β1 protein was obtained from R&D Systems (R&D, USA). Antibodies for α-SMA were obtained from Abcam (Abcam, UK). Antibodies for TGF-β1, MMP2, COL1A1, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and HRP-conjugated secondary antibodies against mouse or rabbit IgG were purchased from Proteintech (Wuhan, China). A rapid block buffer was obtained from New Cell & Molecular Biotech Co., Ltd (Suzhou, China). All other reagents were of analytical grade and were obtained from commercial sources.
Niu X., Wang X., Niu B., Meng Y., He H., Wang Y, & Li G. (2021). Costunolide Loaded in pH-Responsive Mesoporous Silica Nanoparticles for Increased Stability and an Enhanced Anti-Fibrotic Effect. Pharmaceuticals, 14(10), 951.
+ Open protocol
+ Expand
2

Molecular Mechanisms of Cell Signaling

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Protease inhibitor cocktail (P8340), phosphatase inhibitor cocktail (P2850), cycloheximide, MG132 and BrdU were purchased from Sigma-Aldrich. Cell lysis buffer (9803) and antibodies for β-actin, PDGFRβ (1:150), pPDGFRβ (y751/y771, 1:1000), p-LRP6, and p53, Pp53(ser15, 1:3000), p38 (1:200), Pp38(Thr180/Tyr182, 1:1000), JNK (1:500 mg/mL), Phospho-SAPK/JNK (1:1000, Thr183/Tyr185), cyclin D1, ChiP assay kit were all purchased from Cell Signaling Technology. DMEM, fetal bovine serum (FBS), penicillin streptomycin cocktail, Trypsin-EDTA solution, and TRIzol were purchased from GIBCO/Invitrogen; polyvinylidene fluoride membranes from Bio-Rad Laboratories; Antibodies for TCF7L2(sc-8631, Santa Cruz Biotechnology, Inc, 1:200), and protein A/G agarose gel were purchased from Santa Cruz Biotechnology. These TCF7L2 antibody has been previously validated by our group using RNA interference40 (link). Antibodies for α-SMA (1 ug/mL), and SM-MHC (0.4 ug/ml), GATA6 (rabbit, 1:200), p300 (1 µg/ml), p16, were purchased from Abcam, GATA6(goat) antibody was from Novus and CD31 antibody from BD PharMingen, and secondary fluorescence tagged antibodies and propidium iodide from Invitrogen.
Srivastava R., Rolyan H., Xie Y., Li N., Bhat N., Hong L., Esteghamat F., Adeniran A., Geirsson A., Zhang J., Ge G., Nobrega M., Martin K.A, & Mani A. (2018). TCF7L2 (Transcription Factor 7-Like 2) Regulation of GATA6 (GATA-Binding Protein 6)-Dependent and -Independent Vascular Smooth Muscle Cell Plasticity and Intimal Hyperplasia. Arteriosclerosis, Thrombosis, and Vascular Biology, 39(2), 250-262.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!