The bone marrow MSCs were labeled with phycoerythrin-conjugated anti-CD73 (cat no. 550257), fluorescein isothiocyanate (FITC)-conjugated anti-CD90 (cat no. 555595), FITC-conjugated anti-CD105 (cat no. 561443) (all from BD Biosciences, Franklin Lakes, NJ, USA) and human leukocyte antigen G (HLA-G; cat no. 12-9957-73; eBioscience, Inc., San Diego, CA, USA). The fluorescence of the above MSC surface markers was detected and analyzed using flow cytometry with a BD FACSAria™ II system (BD Biosciences). Data were analyzed using the FlowJo software version 7.6 (FlowJo, LLC, Ashland, Oregon, USA).
Fitc conjugated anti cd105
Manufactured by BD
Sourced in United States
FITC-conjugated anti-CD105 is a monoclonal antibody used for the identification and analysis of CD105-positive cells. CD105, also known as endoglin, is a transmembrane glycoprotein expressed on endothelial cells, certain hematopoietic stem cells, and other cell types. The FITC (Fluorescein Isothiocyanate) conjugate allows for the detection of CD105-positive cells using flow cytometry or fluorescence microscopy.
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Lab products found in correlation
Fitc conjugated anti cd90, by BD (2 mentions)
Fitc conjugated anti cd45, by BD (2 mentions)
Pe conjugated anti cd73, by BD (2 mentions)
Anti cd44, by Miltenyi Biotec (2 mentions)
Cd133, by Miltenyi Biotec (2 mentions)
Facsaria 2 system, by BD (1 mentions)
Anti cd73, by BD (1 mentions)
Influx cell sorter, by BD (1 mentions)
Phosphate buffered saline (pbs), by Boster Bio (1 mentions)
Pe conjugated anti cd14, by BD (1 mentions)
Facscalibur flow cytometer, by BD (1 mentions)
Pe conjugated anti cd11b, by BD (1 mentions)
Fitc conjugated anti cd34, by BD (1 mentions)
Accuri, by BD (1 mentions)
Percp cy5.5 conjugated anti cd90, by BD (1 mentions)
Apc conjugated anti cd73, by BD (1 mentions)
Pe conjugated anti cd146, by BD (1 mentions)
Easycyte flow cytometer, by Merck Group (1 mentions)
Pe conjugated anti cd70, by BD (1 mentions)
6 protocols using fitc conjugated anti cd105
1
Phenotypic Characterization of Mesenchymal Stem Cells
2
Flow Cytometry Analysis of MSCs
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MSCs were digested and washed with phosphate-buffered saline (PBS, Boster, Wuhan, China). After incubation with specific antibodies, MSCs were washed again and resuspended in PBS. Flow cytometry was performed using a BD Biosciences Influx Cell Sorter (San Jose, CA, USA). Human phycoerythrin- (PE-) conjugated anti-CD29, fluorescein isothiocyanate- (FITC-) conjugated anti-CD73, FITC-conjugated anti-CD105, FITC-conjugated anti-CD45, PE-conjugated anti-CD14, and PE-conjugated anti-HLA-DR antibodies were used for these experiments (all from BD Biosciences, San Diego, CA, USA).
3
Immunophenotyping of Human BM-MSCs
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Cultured human BM-MSC were harvested with TrypLE Select, and 1 × 106 cells were incubated with primary antibodies for 30 min at 4 °C. The primary antibodies used in this study were FITC-conjugated anti-CD105, PE-conjugated anti-CD73, FITC-conjugated anti-CD90, FITC-conjugated anti-CD45, FITC-conjugated anti-CD34, PE-conjugated anti-CD11b, and PE-conjugated anti-CD14, FITC-conjugated anti-CD19 (BD Bioscience). Cells were washed with blocking reagent and analyzed in a FACS Calibur Flow Cytometer (Beckton Dickinson).
4
Mesenchymal Stem Cell Characterization
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A total of 1.0 × 106 passage 3 cells were incubated per tube with PerCP Cy5.5-conjugated anti-CD90, APC-conjugated anti-CD73, FITC-conjugated anti-CD105, and PE-conjugated anti-CD146 antibodies (all from BD Biosciences, Franklin Lakes, NJ) for 30 minutes, at room temperature, and protected from light. Data were acquired using a BD Accuri flow cytometer (BD Biosciences) and analyzed with the CSampler Accuri Software (BD Biosciences).
5
Stem Cell Marker Expression Profiling
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HeLa, HCT116, and 22Rʋ1 cells were grown in an ultra-low attachment plate for six days with and without treatment of GSK J4. Cells were washed two times in ice-cold PBS and resuspended in 100 µL of staining solution (5 µL PE-conjugated anti-CD 70 (BD Bioscience), CD 133 (Miltenyibiotec-130-090-853), and FITC-conjugated anti-CD 105(BD Bioscience- 56143) and anti-CD44(Miltenyibiotec -130-098-210), 1% FBS, in 1X PBS and incubated at room temperature under the dark condition for 2 h. Then, cells were washed three times in a wash buffer (1%FBS in 1X PBS) and then analyzed by using a Guava Easy-Cyte flow cytometer. For all assays, 10,000 cells were taken for measurement and also for analysis. For each plot, 1000 cells were displayed.
6
Quantification of Cancer Stem Cell Markers
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HeLa, HCT116 and 22R 1 cells were grown in ultra-low attachment plate for six days with and without treatment of GSK J4 and matrix detached cells were washed two times in ice-cold PBS and resuspended in 100 µl of staining solution (5 µl PE-conjugated anti-CD 73 (BD Bioscience), CD 133 (Miltenyibiotec -130-090-853) and FITC conjugated anti-CD 105(BD Bioscience-56143), and anti-CD44(Miltenyibiotec -130-098-210), 1% FBS, in 1X PBS and incubated at room temperature under the dark condition for 2 hours. Then, cells were washed three times in wash buffer (1%FBS in 1X PBS) and then analyzed by Guava Easy-Cyte ow cytometer. For all assays, 10,000 cells were taken for measurement and also for analysis. For each plot 1000 cells were displayed.
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