Nkg2a pe clone z199

Manufactured by Beckman Coulter

The NKG2A PE (clone Z199) is a flow cytometry reagent that is used to detect the expression of the NKG2A receptor on the surface of natural killer (NK) cells and a subset of T cells. The NKG2A receptor is an inhibitory receptor that plays a role in the regulation of the immune response.

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Lab products found in correlation

Cd3 alexa fluor 700 clone sp34 2, by BD (1 mentions) Cd4 brilliant violet 605, by BioLegend (1 mentions) Facsaria 2, by BD (1 mentions) Live dead fixable near ir dead cell stain, by Thermo Fisher Scientific (1 mentions) Tnf α, by R&D Systems (1 mentions)

Close spelling variants of this product

NKG2A-PE (10 citations) NKG2A (Z199, (6 citations) NKG2A (clone Z199 (2 citations) NKG2A PE Cy7 (clone Z199; (2 citations)

2 protocols using nkg2a pe clone z199

Multiparametric flow cytometry panel for immune cell subsets

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PBMCs were thawed and stained with a cocktail of antibodies including CD3 Alexa Fluor 700 (clone SP34-2, BD Pharmingen), CD4 Brilliant Violet 605 (clone OKT4, BioLegend), CD8 Brilliant Violet 785 (clone RPA-T8, BioLegend), CD14 Pacific Blue (clone M5E2, BD Pharmingen), CD16 phycoerythrin (PE)-Cy7 (clone 3G8, BD Pharmingen), CD20 fluorescein isothiocyanate (FITC) (clone 2H7, BD Pharmingen), NKG2A PE (clone Z199, Beckman Coulter), and LIVE/DEAD Aqua viability dye (Life Technologies) to differentiate lymphocyte populations. The stained cells were analyzed and sorted using a BD FACS Aria II, producing pure bulk populations of CD4+ T cells, CD8+ T cells, NK cells, and B cells. The gating strategy is shown in fig. S1.

Ehrenberg P.K., Shangguan S., Issac B., Alter G., Geretz A., Izumi T., Bryant C., Eller M.A., Wegmann F., Apps R., Creegan M., Bolton D.L., Sekaly R.P., Robb M.L., Gramzinski R.A., Pau M.G., Schuitemaker H., Barouch D.H., Michael N.L, & Thomas R. (2019). A vaccine-induced gene expression signature correlates with protection against SIV and HIV in multiple trials. Science translational medicine, 11(507), eaaw4236.

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Multiparametric Immune Profiling Protocol

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All antibodies were purchased from BD Biosciences unless specified otherwise, and clone information is given in parentheses. Antibodies to the following antigens were used in this study: LIVE/DEAD fixable Near-IR Dead Cell stain (Invitrogen), CD3 (clone SP34.2), CCR7 (clone 150503; R&D Systems), CD8α (clone T8/7Pt-3F9, Nonhuman Primate Reagent Resource Program), CD8α (clone SK1), CD16 (clone 3G8), CD56 (clone NCAM16.2), NKG2A-PE (clone Z199; Beckman-Coulter), CD20 (clone 2H7), HLA-DR–Percp (G46-6), CXCR3 (clone IC6), CCR6 (clone 11A9), CCR7 (clone 150503, R&D Systems), CCR4 (clone 1G1), CD32a (clone FL18.26), CD69 (clone FN50), Ki67 (clone B56), perforin (clone Mab-Pf344), granzyme B (GB-11), BCL-6 (clone K112-91), IFN-γ (clone B27), TNF-α (Mab11), CD107a (clone H4A3). See Supplemental Table 2 for more details.

Rahman S.A., Billingsley J.M., Sharma A.A., Styles T.M., Govindaraj S., Shanmugasundaram U., Babu H., Riberio S.P., Ali S.A., Tharp G.K., Ibegbu C., Waggoner S.N., Johnson R.P., Sekaly R.P., Villinger F., Bosinger S.E., Amara R.R, & Velu V. (2022). Lymph node CXCR5+ NK cells associate with control of chronic SHIV infection. JCI Insight, 7(8), e155601.

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