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Gel doc ez documentation system

Manufactured by Bio-Rad
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The Gel Doc EZ Documentation System is a compact, automated imaging system designed for capturing high-quality images of electrophoresis gels and blots. It features a built-in camera and motorized sample tray for simple image acquisition. The system provides accurate, reproducible results for a variety of common lab techniques, including DNA, RNA, and protein gel documentation.

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Lab products found in correlation

Image lab, by Bio-Rad (2 mentions) Qiaamp dna mini kit, by Qiagen (1 mentions) Gotaq green master mix, by Promega (1 mentions)

Close spelling variants of this product

Gel DocTM EZ gel documentation system Gel Doc EZ Gel documentation system Gel Doc system Gel Doc

4 protocols using gel doc ez documentation system

1

Listeria Phage Plaque Assay

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A mixture of 150 μl stationary Listeria culture and 3 mL molten LC top agar (10 g/L tryptone, 5 g/L yeast extract, 10 g/L glucose, 7.5 g/L NaCl, 10 mM CaCl2, 10 mM MgSO4, 0.5% agar) was poured onto a BHI plate (1.5% agar) to generate a bacterial lawn, 3 μL of phage ten-fold serial dilutions were spotted on top, and after 24 hr incubation at 30°C, plate images were collected using the Gel Doc EZ Documentation system (BioRad) and Image Lab (BioRad) software.
Osuna B.A., Karambelkar S., Mahendra C., Sarbach A., Johnson M.C., Kilcher S, & Bondy-Denomy J. (2020). Critical anti-CRISPR locus repression by a bi-functional Cas9 inhibitor. Cell host & microbe, 28(1), 23-30.e5.
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2

Molecular Detection of Malaria Parasites

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The RBC pellet isolated from the small blood volume obtained from each study participant is stored at −80°C until assayed. Upon thawing, DNA is extracted using QIAGEN’s QIAamp DNA mini Kit. A final elution volume of 50 ul is used. Molecular detection of P. falciparum and/or P. vivax is determined by PCR amplifcation of multicopy target loci (Pvr47 present as 14 copies in the P. vivax genome, and Pfr364 present as 41 copies in the P. falciparum genome) as described [41 (link)], GoTaq Green Mastermix (Promega), and a Veriti 96-well Fast Thermal Cycler (Applied Biosytems). PCR products are visualized by gel electrophoresis using ethidium bromide and a Gel Doc EZ documentation system (BioRad). Positive and negative controls are included in all experiments, and 10% of samples are assayed at an independent lab for QC purposes.
Ompad D.C., Kessler A., Van Eijk A.M., Padhan T.K., Haque M.A., Sullivan S.A., Tozan Y., Rocklöv J., Mohanty S., Pradhan M.M., Sahu P.K, & Carlton J.M. (2021). The effectiveness of malaria camps as part of the Durgama Anchalare Malaria Nirakaran (DAMaN) program in Odisha, India: study protocol for a cluster-assigned quasi-experimental study. Global Health Action, 14(1), 1886458.
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3

Detecting Plasmodium Species by PCR

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DNA samples were tested for Plasmodium species by a PCR assay targeting multi-copy loci Pfr364 and Pvr4720 (link) as previously described7 (link). Five μl of DNA was used in a total reaction volume of 30 μl, and amplification products were visually confirmed by ethidium bromide-stained gel electrophoresis using a Gel Doc EZ documentation system (BioRad Laboratories, Inc.).
Ompad D.C., Padhan. T.K., Kessler A., Mohanty S., Tozan Y., Jones A.M., van Eijk A.M., Sullivan S.A., Haque M.A., Pradhan M.M., Mohanty S., Carlton J.M, & Sahu P.K. (2023). The effectiveness of malaria camps as part of the malaria control program in Odisha, India. medRxiv.
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4

Listeria Phage Spot Titer Assay

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A mixture of 150 μL stationary Listeria culture and 3 mL molten LC top agar (10 g/L tryptone, 5 g/L yeast extract, 10 g/L glucose, 7.5 g/L NaCl, 10 mM CaCl2, 10 mM MgSO4, 0.5% agar) was poured onto a BHI plate (1.5% agar) to generate a bacterial lawn, 3 μL of phage ten-fold serial dilutions were spotted on top, and after 24 hr incubation at 30°C, plate images were collected using the Gel Doc EZ Documentation system (BioRad) and Image Lab (BioRad) software.
Osuna B.A., Karambelkar S., Mahendra C., Christie K.A., Garcia B., Davidson A.R., Kleinstiver B.P., Kilcher S, & Bondy-Denomy J. (2020). Listeria phages induce Cas9 degradation to protect lysogenic genomes. Cell host & microbe, 28(1), 31-40.e9.
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