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Beyoclick edu 488 detection kit

Manufactured by Beyotime
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The BeyoClick™ EdU‐488 detection kit is a laboratory tool used to label and detect newly synthesized DNA in cells. It uses a chemical compound called EdU (5-ethynyl-2'-deoxyuridine) that incorporates into DNA during replication. The kit provides a fluorescent dye, Alexa Fluor® 488, which binds to the EdU and allows visualization of the labeled DNA under a fluorescence microscope.

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Lab products found in correlation

Bx53 microscope, by Olympus (1 mentions) Fluorescence microscope, by Olympus (1 mentions)

Close spelling variants of this product

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2 protocols using beyoclick edu 488 detection kit

1

Assessing cell proliferation by EdU staining

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HaCaT keratinocytes were seeded onto 24‐well plate and allowed to grow till 50% confluency. Cells were transfected with miR‐221 mimics and miR‐221 inhibitors. 48h after transfection, the medium was replaced with 10 μM 5‐ethynyl‐2´‐deoxyuridine (EdU) for 2 h. Further, the cells were fixed, permeabilized and stained with EdU (BeyoClick™ EdU‐488 detection kit, Beyotime). Fluorescence was finally visualized with Olympus BX53 microscope (Olympus).
Feng X., Zhang L., Feng W., Zhang C., Jin T., Li J, & Guo J. (2022). miR‐221 promotes keratinocyte proliferation and migration by targeting SOCS7 and is regulated by YB‐1. Journal of Cellular and Molecular Medicine, 26(8), 2299-2311.
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2

EdU Assay for Cell Proliferation

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The EdU assay was performed following the guidelines provided by the BeyoClick EdU-488 Detection Kit (Beyotime). Cells were initially seeded in 6-well plates and cultured for 24 h. Subsequently, they were incubated with EdU solution at the concentration of 10 μM for 2 h in a cell incubator. Then, cells were fixed in 4% paraformaldehyde, permeabilized with 0.3% Triton, and stained with reaction buffer and Hoechst33342. The observation and photography of cells were carried out under a fluorescence microscope (Olympus).
Wang R., Su D., Liu Y., Huang H., Qiu J., Cao Z., Yang G., Chen H., Luo W., Tao J., Weng G, & Zhang T. (2024). The NF-κB/NUAK2 signaling axis regulates pancreatic cancer progression by targeting SMAD2/3. iScience, 27(4), 109406.
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