Mouse anti il 10

The following primary antibodies were used: anti-BrdU monoclonal antibodies (1:400, Oxford Biotechnology, UK), goat anti-DCX (1:400; Santa Cruz Biotechnology, CA, USA), rabbit anti-BDNF (1:200; Abcam, Cambridge, MA, USA), goat anti-mouse IGF-1 (1:200; BD Bioscience, San Jose, CA, USA), rat anti-mouse anti-CD3 (eBioscience, Santa Clara, CA, USA), rabbit anti-Iba-1 (1:1000; Wako Chemical, Japan), mouse anti-GFAP (1:10,000; Sigma-Aldrich, St. Louis, MO, USA), mouse anti-IL-10 (1:200; BD Bioscience, San Jose, CA, USA), chicken anti-Arginase-1 (1:500; Merck Millipore, Germany), rat anti-CD11b (1:1000; BD Bioscience, San Jose, CA, USA), and mouse anti-NeuN (1:1000; Sigma-Aldrich, St. Louis, MO, USA). The following secondary antibodies were used: Alexa Fluor 594 donkey anti-rat, Alexa Fluor 488 donkey anti-goat, Alexa Fluor 555 goat anti-rabbit, Alexa Fluor 488 goat anti-rabbit, Alexa Fluor FITC goat anti-chicken, and Alexa Fluor 488 goat anti-mouse antibodies (1:400; Molecular Probes, Eugene, OR, USA). The detailed immunofluorescence protocols were previously described [23 (link)].