Western blot analysis was performed using standard methods. The antibodies used were as follows: RhoGDIα (1:1000; Abcam, USA), P-LIMK (1:1000; CST, USA), LIMK (1:1000; Proteintech, USA), P-cofilin (1:1000; CST, USA), cofilin (1:1000; Proteintech, USA), P53 (1:1000; CST, USA), Rac1 (1:1000; Proteintech, USA), Cdc42 (1:1000; Proteintech, USA), Pak4 (1:1000; Proteintech, USA), Rock1 (1:1000; Proteintech, USA), RhoA (1:1000; Affinity, China) and GAPDH (1:10000; Proteintech, USA). Rac1 and RhoA activity was detected by the Rac1/RhoA Pull-Down Activation Assay Biochem Kit (Cytoskeleton, USA). The Western blot bands were quantified by ImageJ software and normalized to GAPDH.
Cdc42
Manufactured by Proteintech
Sourced in United States
Cdc42 is a small GTPase protein that regulates various cellular processes, including cell division, cell migration, and cytoskeleton organization. It acts as a molecular switch, cycling between an active GTP-bound state and an inactive GDP-bound state. Cdc42 is a core component of cellular signaling pathways and plays a crucial role in the regulation of cellular functions.
Automatically generated - may contain errors
Lab products found in correlation
Cofilin, by Proteintech (1 mentions)
Rock1, by Proteintech (1 mentions)
Rhogdiα, by Abcam (1 mentions)
Gapdh, by Proteintech (1 mentions)
Caspase 3, by Cell Signaling Technology (1 mentions)
P44 42 mapk erk1 2, by Cell Signaling Technology (1 mentions)
P mek, by Cell Signaling Technology (1 mentions)
Pd98059, by MedChemExpress (1 mentions)
β tubulin, by Cell Signaling Technology (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
Bcl2 associated x (bax), by Proteintech (1 mentions)
U0126, by MedChemExpress (1 mentions)
Bcl 2, by Proteintech (1 mentions)
Dihydroartemisinin dha, by Merck Group (1 mentions)
Vascular endothelial growth factor (vegf), by Abcam (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Active cdc42 detection kit, by Cell Signaling Technology (1 mentions)
Antibodies against p65, by Cell Signaling Technology (1 mentions)
Ml141, by Selleck Chemicals (1 mentions)
Cortactin, by Proteintech (1 mentions)
4 protocols using cdc42
1
Comprehensive Protein Analysis Protocol
2
Investigating EGFR-Mediated Signaling Pathways
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Que was purchased from Sigma Aldrich (Merck KGaA). The following primary antibodies were used in the present study: phosphorylated (p)-EGFR (Tyr1068) (1:1,000; cat. no. 3777; Cell Signaling Technology, Inc.), p-MEK (1:1,000; cat. no. 2338; Cell Signaling Technology, Inc.), p-cell division cycle (Cdc) 42 (1:1,000; cat. no. 2461; Cell Signaling Technology, Inc.), EGFR (1:1,000; cat. no. 4267; Cell Signaling Technology, Inc.), MEK (1:1,000; cat. no. 9122; Cell Signaling Technology, Inc.), p-44/42 MAPK (ERK1/2) (1:1,000; cat. no. 4370; Cell Signaling Technology, Inc.), caspase-3 (1:1,000; cat. no. 9664; Cell Signaling Technology, Inc.), GAPDH (1:3,000; cat. no. 5174; Cell Signaling Technology, Inc.), β-tubulin (1:3,000; cat. no. 10094-1-AP; ProteinTech Group, Inc.), cdc42 (1:1,000; cat. no. 10155-1-AP; ProteinTech Group, Inc.), Bax (1:1,000; cat. no. 50599-2-lg; ProteinTech Group, Inc.) and Bcl-2 (1:1,000; cat. no. 12789-1-AP; ProteinTech Group, Inc.). The secondary antibodies, HRP-conjugated goat-anti-rabbit (cat. no. L3012) and goat-anti-mouse (cat. no. L3032) IgG were purchased from Signalway Antibody LLC. The small molecule inhibitors, aftinib, PD98059, U0126, ML141 for EGFR, MEK, ERK and cdc42 were purchased from MedChemExpress.
3
Dihydroartemisinin Signaling Pathway Assay
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Dihydroartemisinin (DHA) was purchased from Sigma-Aldrich, Inc. (St. Louis, MO, USA), dissolved in DMSO to make a 1000 mM stock solution, and stored at 4°C. The following antibodies were used: β-actin, Cdk4, Cdk6 (Santa Cruz Biotechnology, Carlsbad, CA, USA), VEGF (Abcam Inc., Cambridge, MA, USA), IKKα, MEK1 (Cell Signalling Technology, Inc., Danvers, MA, USA), E2F3, Rac1, E2F1, and CDC42 (Proteintech, Chicago, IL, USA).
4
Antibody Validation and Cellular Signaling Assays
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Antibody against CIP4 (Cat:612556) was purchased from BD Biosciences (NY, USA). Antibodies against GAPDH (Cat:60004), Cdc42 (Cat:10155), Cortactin (Cat:11381), Histone H3 (Cat:17168), FLAG tag (Cat:66008) were purchased from Proteintech (Illinois, USA). Antibodies against p65 (Cat:8242), p-p65 (Cat:3033), the Active Cdc42 Detection Kit (Cat: 8819) were purchased from Cell Signaling Technology (Massachusetts, USA). Antibodies against His tag (Cat:0287R) and GST tag (Cat:33007M) were purchased from Bioss (Beijing, China). The Cdc42 inhibitor ML141 and the NF-κB signaling inhibitor QNZ were purchased from Selleck Chemicals (Shanghai, China). The NF-κB signaling activator LPS was purchased from Sigma (Missouri, USA).
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