Log In Sign up
The largest database of trusted experimental protocols

Lsm 510 meta

Manufactured by Nikon
Visit Supplier

The LSM 510 META is a laser scanning microscope designed for advanced microscopy applications. It features a multi-wavelength laser system, enabling the examination of multiple fluorescent specimens simultaneously. The LSM 510 META provides high-resolution imaging and offers flexible configuration options to meet the needs of various research and imaging applications.

Automatically generated - may contain errors

Lab products found in correlation

S8305, by Merck Group (1 mentions) Cm1950, by Leica (1 mentions) Cy3 streptavidin, by Jackson ImmunoResearch (1 mentions) Alexa fluor 488, by Jackson ImmunoResearch (1 mentions) Cyanine3 (cy3), by Jackson ImmunoResearch (1 mentions) Ab144p, by Merck Group (1 mentions) Desmin, by Agilent Technologies (1 mentions) P camkii, by Abcam (1 mentions) Adamts13, by Abcam (1 mentions) A1r confocal microscope, by Nikon (1 mentions) A1rsi, by Nikon (1 mentions)

Close spelling variants of this product

LSM 510 (16 citations) UV-LSM 510 META (3 citations) LSM 510 Meta microscope (3 citations) LSM 510 META Laser Scanning Confocal Microscopy (2 citations)

6 protocols using lsm 510 meta

1

Immunohistochemical Analysis of Cholinergic Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols
Adult mice were anesthetized with an overdose of pentobarbital and then transcardially perfused with 4% paraformaldehyde (PFA). Mouse brain was dissected and fixed in 4% PFA for 4 h. After cryoprotection in 30% sucrose, brain sections (35 μm) were cut on a cryostat microtome (Leica CM1950, Leica Biosystems, Wetzlar, Germany). After rinsing with PBS and 0.3% Triton-X in 0.1 M PBS (PBST), the brain sections were blocked with 2% (w/v) bovine serum (BSA) in PBST for 1 h. Then, the brain sections were incubated with primary antibodies at 4°C for 48 h and secondary antibodies at room temperature for 2 h. Images were collected using a Zeiss LSM510 Meta or Nikon A1 confocal microscope and analyzed using FIJI. The antibodies used were as follows: anti-choline acetyltransferase (1:200, goat, AB144P, MERCK, Kenilworth, NJ, United States), anti-NK1R (S8305, rabbit, 1:5,000, Sigma-Aldrich, St. Louis, MO, United States), goat anti-rabbit for NK1R (Cy3 and Alexa Fluor 488, Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, United States), donkey anti-goat for choline acetyltransferase (Cy3 and Alexa Fluor 488, Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, United States), and Cy3-streptavidin (1:500).
Ren Y., Liu Y, & Luo M. (2021). Gap Junctions Between Striatal D1 Neurons and Cholinergic Interneurons. Frontiers in Cellular Neuroscience, 15, 674399.
+ Open protocol
+ Expand
2

Spinal Cord Immunohistochemistry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were cardiac-perfused with 4% paraformaldehyde in PBS. Spinal cords were dissected and immersed in 4% paraformaldehyde in PBS overnight, washed in PBS and submerged in 30% sucrose. Tissues were embedded in O.C.T. for cryosectioning at 30 μm collected onto slides. Slides were incubated with primary antibody in 1% goat or donkey serum/0.3% Triton X-100/PBS at 4°C overnight, followed by incubation with secondary antibody conjugated with Alexa Fluor 488, 568 or 647 (Molecular Probes). For spinal cord sections subjected for BrdU staining, dsRed antibody was use along with Alexa Fluor 568 secondary to amplify the fluorescent of tdTOM. Sections were then fixed in 4% PFA/PBS for 5 minutes, treated with 2M HCl/0.2% Triton-X100 for 20 minutes to exposed BrdU epitope, then incubated with anti-BrdU overnight, followed by Alexa Fluor secondary the following day to detect BrdU+;tdTOM+ cells. All slides were mounted with Vectashield mounting medium (#101098-042) and imaged using a Zeiss LSM 510 META and Nikon ECLIPSE 80i Upright fluorescent microscopes. The following antibodies were used for this study:
Kelenis D.P., Hart E., Edwards‐Fligner M., Johnson J.E, & Vue T.Y. (2018). ASCL1 regulates proliferation of NG2‐glia in the embryonic and adult spinal cord. Glia, 66(9), 1862-1880.
+ Open protocol
+ Expand
3

Multimodal Protein Profiling in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
After the stimulation period, cells were fixed in 4% PFA, stained for specific antigens using primary antibodies against desmin (DAKO), DDR2 (Santa Cruz), NCX (SWANT), P4HB, Vimentin, P‐CaMKII, ADAMTS13 (Abcam), incubated with fluorophore‐conjugated secondary antibodies and visualized on Zeiss LSM 510 Meta and Nikon A1 confocal microscopes. The images were analyzed using ImageJ software (ImageJ 1.46r, NIH, USA).
Djalinac N., Ljubojevic‐Holzer S., Matzer I., Kolesnik E., Jandl K., Lohberger B., Rainer P., Heinemann A., Sedej S., von Lewinski D, & Bisping E. (2020). The role of stretch, tachycardia and sodium‐calcium exchanger in induction of early cardiac remodelling. Journal of Cellular and Molecular Medicine, 24(15), 8732-8743.
+ Open protocol
+ Expand
4

Visualizing Mitochondrial Morphology

Check if the same lab product or an alternative is used in the 5 most similar protocols
After 24 hours of transfection, the cells were fixed with 4 % Paraformaldehyde in PBS. Following permeabilization with PBS containing 0.1 % Triton, 1 % BSA and 2 % Horse serum cells were incubated with anti-Myc primary monoclonal antibody (Covince; CAT#MMS-150P) overnight at 4°C. After washing the cells extensively with PBS, secondary anti-mouse FITC conjugated was added to the cells for 1h at room temperature. The staining was then visualized using a Carl Zeiss LSM510 META or a Nikon A1R confocal microscope. To quantify mitochondrial morphology, a custom ImageJ macro was utilized as previously described (9 (link)). Statistical analysis was performed using the unpaired Student's t-test and values of p<0.05 were considered significant.
Ajroud-Driss S., Fecto F., Ajroud K., Lalani I., Calvo S.E., Mootha V.K., Deng H.X., Siddique N., Tahmoush A.J., Heiman-Patterson T.D, & Siddique T. (2014). Mutation in the Novel Nuclear-Encoded Mitochondrial Protein CHCHD10 in a Family with Autosomal Dominant Mitochondrial Myopathy. Neurogenetics, 16(1), 1-9.
+ Open protocol
+ Expand
5

Live Cell Imaging of eGFP-RTA Variants

Check if the same lab product or an alternative is used in the 5 most similar protocols
Live cell imaging of eGFP-RTAE177D or eGFP-RTAE177D-HDEL was performed by confocal fluorescence microscopy using a Zeiss LSM 510 META (Nikon PlanApo 63x NA 1.4 oil immersion lens, 488 nm excitation, 1.1% argon laser power, HFT 488 and NFT 490 beam splitter, BP 500-530 filter). The same laser power and pinhole size (73 μm) were used to collect all images in each experiment.
Bauer A., Santen L., Schmitt M.J., Shaebani M.R, & Becker B. (2020). Cell-type-specific differences in KDEL receptor clustering in mammalian cells. PLoS ONE, 15(7), e0235864.
+ Open protocol
+ Expand
6

Visualizing Plant Organelle Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols
A Zeiss LSM 510 META and a Nikon A1Rsi laser scanning confocal microscope were used for imaging. Acquired images were handled by NIS-Elements Advanced Research (Nikon), ZEN (Zeiss) and Fiji (ImageJ) (Schindelin et al., 2012 (link)). The fluorescent protein fusions used in this study are GFP-δTIP (Cutler et al., 2000 (link)), ERYK (Nelson et al., 2007 (link)), YFP-ABD2 (Sheahan et al., 2004 (link)), GFP-CASP (Renna et al., 2005 (link)), SEC-RFP (Faso et al., 2009 (link)) and γTIP-YFP (Nelson et al., 2007 (link)). Transformation of Arabidopsis plants were conducted using floral dip method (Clough and Bent, 1998 (link)).
Cao P., Kim S.J., Xing A., Schenck C.A., Liu L., Jiang N., Wang J., Last R.L, & Brandizzi F. (2019). Homeostasis of branched-chain amino acids is critical for the activity of TOR signaling in Arabidopsis. eLife, 8, e50747.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!