Ab2917

Manufactured by Abcam

Sourced in Denmark, United States

Ab2917 is an antibody detection product. It is designed for use in various laboratory techniques.

Automatically generated - may contain errors

Lab products found in correlation

Anti ubiquitin, by Agilent Technologies (1 mentions) Triton x 100, by Bio-Rad (1 mentions) B0586, by Abcam (1 mentions) Ab2787, by Abcam (1 mentions) Ab8637, by Abcam (1 mentions) Tcs sp8 confocal microscope, by Leica (1 mentions) Ab25630, by Abcam (1 mentions) Hrp conjugated secondary antibody, by Bio-Rad (1 mentions) Image lab software, by Bio-Rad (1 mentions) Chemidoc imaging system, by Bio-Rad (1 mentions) Anti sod2, by Santa Cruz Biotechnology (1 mentions) Immobilion p, by Merck Group (1 mentions) Ab14744, by Abcam (1 mentions) Anti α tubulin, by Merck Group (1 mentions) Anti β actin c4, by Santa Cruz Biotechnology (1 mentions) Clarity western ecl substrate, by Bio-Rad (1 mentions)

3 protocols using ab2917

Neuropathological Examination of Rare Fatal Conditions

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Three patients died during follow-up (III-10, III-12, and III-13); brain autopsy was performed by the Netherlands Brain Bank within 4 hours after death. Formalin-fixed (10%) and paraffin-embedded tissue blocks were available for examination. Eight-micrometer sections of all cortical regions, subcortical nuclei, brainstem, and cerebellum underwent routine staining. Immunohistochemistry was performed using the following antibodies: hyperphosphorylated tau (AT8, Innogenetics, Ghent, Belgium; 1:40), beta-amyloid (anti-amyloid, DAKO, Glostrup, Denmark; 1:100, following formic acid pretreatment), alpha-synuclein (anti-synuclein, Zymed Laboratories, San Francisco, CA; undiluted, following formic acid pretreatment), TDP-43 (anti-phospho TDP-43, Cosmo Bio, 1:100 and Proteintech Group, 1:100), ubiquitin (anti-ubiquitin, DAKO, Glostrup, Denmark; 1:500, following 80°C antigen retrieval), p62 (mouse D3 Clone, Santa Cruz, 1:100), 1C2 (mouse 5TF1-1C2-172 Clone, Chemicon, 1:3,200), and STUB1 (rabbit anti-STUB1 Abcam ab2917, 1:100).

Mol M.O., van Rooij J.G., Brusse E., Verkerk A.J., Melhem S., den Dunnen W.F., Rizzu P., Cupidi C., van Swieten J.C, & Donker Kaat L. (2020). Clinical and pathologic phenotype of a large family with heterozygous STUB1 mutation. Neurology: Genetics, 6(3), e417.

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Hepatitis B Virus Degradation Pathways

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HepAD38 cells were infected with LV-sh_STUB1 or LV-contr. for 24 h and then treated with 1 μM Bay41-4109 for 48 h, 0.1 μM Baf A1 for 12 h, 10 mM 3-MA for 12 h, or a combination of them as indicated. The cells were washed with PBS, fixed in 4% paraformaldehyde, and permeabilized with 0.1% Triton X-100 (Bio-Rad). The cells were then stained with anti-HBc (1:1000, Dako, B0586), anti-p62 (1:50, abcam, ab256416), anti-LAMP1 (1:40, abcam, ab25630), or anti-Hsp70 (1:200, abcam, ab2787) followed by incubation with AlexaFluor 594-, AlexaFluor 488-, or DyLight 405-conjugated secondary antibodies (cell signaling, #8890 or #4408; abbkine, A23120) for the co-localization of HBc with LAMP1. For the double staining of HBc and STUB1, the primary antibodies anti-HBc (1:100, abcam, ab8637) and anti-STUB1 (1:200, abcam, ab2917) were used. Fluorescent images were acquired using a Leica TCS SP8 confocal microscope.

Lin J., Yin L., Xu X.Z., Sun H.C., Huang Z.H., Ni X.Y., Chen Y, & Lin X. (2022). Bay41-4109-induced aberrant polymers of hepatitis b capsid proteins are removed via STUB1-promoted p62-mediated macroautophagy. PLoS Pathogens, 18(1), e1010204.

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Western blot analysis of cellular proteins

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Protein extracts (50 μg) were loaded on an SDS-PAGE and transferred onto a polyvinylidene fluoride (PVDF) membrane (Immobilion-P, Millipore). After blocking in 3% BSA, membranes were incubated with the following primary antibodies: anti-β-Actin (C4) (sc-47778, Santa Cruz Biotechnology, Dallas, TX, USA), anti-TERT (600-401-252S, Rockland), anti-SOD2 (sc-137254, Santa Cruz Biotechnology), anti-Aco2 (NBP1-32781, Novus Biological), anti-CHIP (ab2917, Abcam), anti-COX IV (ab14744, Abcam) and anti-α-tubulin (T6199, Sigma-Aldrich). Finally, membranes were incubated with the appropriate HRP-conjugated secondary antibody (Bio-Rad Laboratories, Hercules, CA, USA). Proteins were visualized using Clarity^TM Western ECL substrates (Bio-Rad). Images were acquired on ChemiDoc™ Imaging system (Bio-Rad) and protein levels were quantified using the Image Lab software (Bio-Rad). Experiments were repeated at least three times.

Marinaccio J., Micheli E., Udroiu I., Di Nottia M., Carrozzo R., Baranzini N., Grimaldi A., Leone S., Moreno S., Muzzi M, & Sgura A. (2023). TERT Extra-Telomeric Roles: Antioxidant Activity and Mitochondrial Protection. International Journal of Molecular Sciences, 24(5), 4450.

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