Log In Sign up
The largest database of trusted experimental protocols

Maxima sybr green rox qpcr master mix assay

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States

Maxima SYBR Green/ROX qPCR Master Mix is a ready-to-use solution for real-time quantitative PCR (qPCR) experiments. It contains SYBR Green I dye and ROX passive reference dye, as well as all the necessary components for efficient qPCR amplification and detection.

Automatically generated - may contain errors

Lab products found in correlation

Rneasy mini kit, by Qiagen (7 mentions) Steponeplus system, by Thermo Fisher Scientific (7 mentions) Steponeplus real time pcr system, by Thermo Fisher Scientific (6 mentions) High capacity cdna archive kit, by Thermo Fisher Scientific (5 mentions) Trizol reagent, by Thermo Fisher Scientific (5 mentions) Quantitect reverse transcription kit, by Qiagen (4 mentions) Qscript microrna cdna synthesis kit, by Quanta Biosciences (3 mentions) Ncode vilo mirna cdna synthesis kit, by Thermo Fisher Scientific (2 mentions) Mirvana mirna isolation kit, by Thermo Fisher Scientific (2 mentions) Quantitect reverse trancription kit, by Qiagen (1 mentions)

14 protocols using maxima sybr green rox qpcr master mix assay

1

Quantitative RT-PCR for Sod2 Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
mRNA was isolated from E8.75 embryos using the Trizol reagent (Invitrogen, Calsbad, CA), and then reversed transcribed using the high-capacity cDNA archive kit (Applied Biosystem, Grand Island, NY). RT-PCR for Sod2 and β-actin were performed using the Maxima SYBR Green/ROX qPCR Master Mix assay (Thermo Scientific, Rockford, IL) in the StepOnePlus system (Applied Biosystem, Grand Island, NY). Primer sequences are listed below, Sod2, forward: 5’-GTTGGGGTTGGCTTGGTTT-3’; reverse: 5’- TAAGGCCTGTTGTTCCTTG-3’; β-actin, forward: 5’-GTGACGTTGACATCCGTAAAGA-3’ reverse: 5’-GCCGGACTCATCGTACTCC-3’.
Zhong J., Xu C., Gabbay-Benziv R., Lin X, & Yang P. (2016). Superoxide dismutase 2 overexpression alleviates maternal diabetes-induced neural tube defects, restores mitochondrial function and suppresses cellular stress in diabetic embryopathy. Free radical biology & medicine, 96, 234-244.
+ Open protocol
+ Expand
2

Quantitative Analysis of miRNA and mRNA Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated from embryos or cultured cells using the mirVana miRNA isolation kit (Ambion) and reverse transcribed using the NCode VILO miRNA cDNA synthesis kit (Invitrogen). Real-time PCR for CITED2, β-actin, miR-200b, and small nuclear RNA U6 was performed using Maxima SYBR Green/ROX qPCR Master Mix assay (Thermo Scientific). Real-time PCR and subsequent calculations were performed by a StepOnePlus Real-Time PCR System (Applied Biosystem). Real-time PCR primer sequences are listed in Supplementary Table 3.
Gu H., Yu J., Dong D., Zhou Q., Wang J.Y., Fang S, & Yang P. (2015). High Glucose–Repressed CITED2 Expression Through miR-200b Triggers the Unfolded Protein Response and Endoplasmic Reticulum Stress. Diabetes, 65(1), 149-163.
+ Open protocol
+ Expand
3

Quantification of SIRT1-7 Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated from embryos or cultured cells using the mirVana miRNA isolation kit (Ambion, Life Technologies, Grand Island, NY) and reverse transcribed using the NCode VILO miRNA cDNA synthesis kit (Invitrogen, Life Technologies, Grand Island, NY). RT-PCR for SIRT1-7 and β-actin were performed using the Maxima SYBR Green/ROX qPCR Master Mix assay (Thermo Scientific). RT-PCR and subsequent calculations were performed by a StepOnePlus™ Real-Time PCR System (Applied Biosystem, Foster City, CA).
Yu J., Wu Y, & Yang P. (2016). High glucose-induced oxidative stress represses sirtuin deacetylase expression and increases histone acetylation leading to neural tube defects. Journal of neurochemistry, 137(3), 371-383.
+ Open protocol
+ Expand
4

Quantifying HIF1a, VEGF Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Using the TRIzol reagent, mRNA was isolated from each ovary, and then reversed transcribed using the high-capacity cDNA archive kit (Applied Biosystem, Grand Island, NY). RT-PCR for HIF1a, VEGF and β-actin were performed using the Maxima SYBR Green/ROX qPCR Master Mix assay (Thermo Scientific, Rockford, IL) in the StepOne Plus system (Applied Biosystem).
Wu Y., Li Y., Liao X., Wang Z., Li R., Zou S., Jiang T., Zheng B., Duan P, & Xiao J. (2017). Diabetes Induces Abnormal Ovarian Function via Triggering Apoptosis of Granulosa Cells and Suppressing Ovarian Angiogenesis. International Journal of Biological Sciences, 13(10), 1297-1308.
+ Open protocol
+ Expand
5

Quantitative Transcriptional Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated from tissues and cells using TRIzol reagent (Ambion) and reverse-transcribed using the QuantiTect Reverse Transcription Kit (Qiagen). Reverse transcription for miRNA was performed using the qScript microRNA cDNA Synthesis Kit (Quanta Biosciences). Quantitative reverse transcription polymerase chain reaction (RT-qPCR) of mRNA, pri-miRNA, and small nuclear RNA U6 was performed using the Maxima SYBR Green/ROX qPCR Master Mix assay (Thermo Fisher Scientific). The primers for RT-qPCR are listed in table S11. RT-qPCR and subsequent calculations were performed by a StepOnePlus Real-Time PCR System (Applied Biosystem).
Xu C., Shen W.B., Reece E.A., Hasuwa H., Harman C., Kaushal S, & Yang P. (2021). Maternal diabetes induces senescence and neural tube defects sensitive to the senomorphic rapamycin. Science Advances, 7(27), eabf5089.
+ Open protocol
+ Expand
6

Cardiac Hypertrophy and Remodeling Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
To demonstrate the expression of hypertrophy and cardiac remodeling markers in messenger RNA level, mRNA was isolated from E17.5-day hearts and H9C2 cells using the RNeasy Mini Kit (Qiagen, Hilden, Germany), and reversed transcribed using the High-Capacity cDNA Archive Kit (Applied Biosystems, Grand Island, NY, USA). Real-time qPCR for β-myosin heavy chain (β-MHC), atrial natriuretic peptide (ANP), insulin-like growth factor 1 (IGF1), desmin (DES), adrenomedullin (ADM), connective tissue growth factor (CTGF), osteopontin (OPN), galectin-3 (GAL)3, MMP1, MMP13, TIMP1, TIMP2, TIMP3, TIMP4 COL1a, COL4a, FN1 and β-Actin were performed using the Maxima SYBR Green/ROX qPCR Master Mix Assay (Thermo Fisher Scientific, Waltham, MA, USA) in the StepOnePlus system (Applied Biosystems, Foster City, CA, USA). The primer sequences used are listed in Supplementary Table 1.
Lin X., Yang P., Reece E.A, & Yang P. (2017). Pregestational type 2 diabetes mellitus induces cardiac hypertrophy in the murine embryo through cardiac remodeling and fibrosis. American journal of obstetrics and gynecology, 217(2), 216.e1-216.e13.
+ Open protocol
+ Expand
7

Quantitative Analysis of Cardiac TGF-β Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated from E12.5 embryonic hearts using an RNeasy Mini Kit (Qiagen, Valencia, CA). Real-time PCR for TGFβ1, β2, β3, Snai2 (snail homolog 2), CTGF (connective tissue growth factor), GDF1 (growth differentiation factor 1) and β-actin were performed using Maxima SYBR Green/ROX qPCR Master Mix assay (Thermo Scientific, Rockford, US). RT-PCR and subsequent calculations were performed by a 7700 ABI PRISM sequence detector system (Applied Biosystem). Primer sequences for RT-PCR are listed in Table 1.
Wang F., Reece E.A, & Yang P. (2015). Oxidative stress is responsible for maternal diabetes-impaired transforming growth factor beta signaling in the developing mouse heart. American journal of obstetrics and gynecology, 212(5), 650.e1-650.e11.
+ Open protocol
+ Expand
8

Quantitative RT-PCR Analysis of BMP4 Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total mRNA was isolated from E8.5 embryos using the RNeasy Mini kit (Qiagen, Hilden, Germany) and mRNA was reverse transcribed to cDNA using the Quanti Tect Reverse Transcription Kit (Qiagen, Hilden, Germany). Real time-PCR for Bmp4, Id1, Id2, Id3, Id4, Smad6, and β-actin were performed using the Maxima SYBR Green/ROX qPCR Master Mix assay (Thermo Fisher Scientific; Waltham, MA) in the Step One Plus system (Applied Biosystems, Grand Island, NY). The primers for RT-PCR are listed in Table S2.
Cao S., Reece E.A., Shen W.B, & Yang P. (2020). Restoring BMP4 expression in vascular endothelial progenitors ameliorates maternal diabetes-induced apoptosis and neural tube defects. Cell Death & Disease, 11(10), 859.
+ Open protocol
+ Expand
9

RNA Extraction and RT-qPCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated from cells or embryonic tissue using the RNeasy mini kit and reverse transcribed using the QuantiTect reverse transcription kit (Qiagen, Hilden, Germany). Real-time polymerase chain reaction (RT-qPCR) was performed using the Maxima SYBR Green/ROX qPCR Master Mix assay (Thermo Scientific Danvers, MA). The primers for RT-qPCR are listed in Table 1. RT-qPCR and subsequent calculations were performed by a StepOnePlus Real-Time PCR System (Applied Biosystems, Foster City, CA).
Xu C., Chen X., Reece E.A., Lu W, & Yang P. (2018). The increased activity of a transcription factor inhibits autophagy in diabetic embryopathy. American journal of obstetrics and gynecology, 220(1), 108.e1-108.e12.
+ Open protocol
+ Expand
10

Brain Tissue RNA Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated from brain tissue using the Trizol reagent (Ambion) and reverse transcribed using the QuantiTect Reverse Trancription Kit (Qiagen). RT-qPCR for ER chaperone genes and GAPDH was performed using the Maxima SYBR Green/ROX qPCR Master Mix assay (Thermo Scientific). The primers for RT-qPCR are listed in Table 2. RT-qPCR and subsequent calculations were performed by a StepOnePlus™ Real-Time PCR System (Applied Biosystem).
Dong D., Zielke R., Yeh D, & Yang P. (2018). Cellular stress and apoptosis contribute to the pathogenesis of autism spectrum disorder. Autism research : official journal of the International Society for Autism Research, 11(7), 1076-1090.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!