Sc 271269

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Sc-271269 is a laboratory instrument used for the analysis and detection of various biological samples. It is designed to perform specific functions within the scientific research and clinical diagnostic fields. The core function of this product is to facilitate the identification and quantification of target analytes in a consistent and reliable manner. Further details on its intended use or performance characteristics are not available.

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Lab products found in correlation

Nitrocellulose membrane, by GE Healthcare (1 mentions) Hpa001240, by Merck Group (1 mentions) Sc 7383, by Santa Cruz Biotechnology (1 mentions) Sc 55572, by Santa Cruz Biotechnology (1 mentions) T0198, by Merck Group (1 mentions) Total erk, by Santa Cruz Biotechnology (1 mentions) Dc assay, by Bio-Rad (1 mentions) P erk, by Santa Cruz Biotechnology (1 mentions) G box chemi xt4, by Syngene (1 mentions) Clarity western ecl substrate, by Bio-Rad (1 mentions) Trans blot sd, by Bio-Rad (1 mentions) Immobilon p, by Merck Group (1 mentions) M9692, by Merck Group (1 mentions) Enhanced chemi luminescence (ecl), by Cytiva (1 mentions) Ab137474, by Abcam (1 mentions) Ab53096, by Abcam (1 mentions) Ab12169, by Abcam (1 mentions) Sc 14939, by Santa Cruz Biotechnology (1 mentions) Sc 146, by Santa Cruz Biotechnology (1 mentions) Ab34710, by Abcam (1 mentions)

3 protocols using sc 271269

Western Blot Analysis of Protein Targets

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Untreated or specifically treated cells were washed twice with cold PBS and harvested in RIPA lysis buffer supplemented with 1% protease inhibitor. Cells were scrapped, lysates were collected, homogenized with syringe and needle, and centrifuged at 30,000 x g for 5 min at 4 °C. Clear lysate was transferred to a new tube. Protein concentration of lysate was determined using the DC assay (BioRad). Protein samples were resolved by SDS-PAGE and transferred to a nitrocellulose membrane (GE Healthcare).
Primary antibodies used: CBS (1:1000, sc-133154, Santa Cruz Biotechnology), MPST (1:4000, HPA001240, Sigma Aldrich), CTH (MEF cells, 1:4000), CTH (HeLa cells, 1:1000, sc-374249, Santa Cruz Biotechnology), p-ERK (1:1000, sc-7383, Santa Cruz Biotechnology), total-ERK (1:1000, sc-271269, Santa Cruz Biotechnology), DJ-1 (1:250, sc-55572, Santa Cruz Biotechnology), DJ-1 Oxidized At C106 (1:1000, HCA024, BioRad) and β-tubulin (1:5000, T0198, Sigma Aldrich). Species-specific horseradish-conjugated secondary antibodies (1:5000, Santa Cruz Biotechnology) were used for antigen detection and visualized using Clarity™ Western ECL Substrate (BioRad) on a G:Box Chemi-XT4 (Syngene).

Zivanovic J., Kouroussis E., Kohl J.B., Adhikari B., Bursac B., Schott-Roux S., Petrovic D., Miljkovic J.L., Thomas-Lopez D., Jung Y., Miler M., Mitchell S., Milosevic V., Gomes J.E., Benhar M., Gonzalez-Zorn B., Ivanovic-Burmazovic I., Torregrossa R., Mitchell J.R., Whiteman M., Schwarz G., Snyder S.H., Paul B.D., Carroll K.S, & Filipovic M.R. (2019). SELECTIVE PERSULFIDE DETECTION REVEALS EVOLUTIONARILY CONSERVED ANTI-AGING EFFECTS OF S-SULFHYDRATION. Cell metabolism, 30(6), 1152-1170.e13.

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Endogenous and Recombinant ERK1 Expression in Mouse Eggs

Cited 1 time

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To assess for the presence of endogenous and recombinant expression of ERK1, mouse eggs were collected in SDS buffer, heated for 5 min at 100°C and proteins were separated by SDS-PAGE (Nomikos et al., 2011 (link)). Immunoblotting was then performed as described previously (Verlhac et al., 1996 (link)). Following transfer onto polyvinylidene difluoride membrane (Immobilon-P; Millipore) using a semi-dry transfer system (Trans-Blot SD; Bio-Rad) in buffer (48 mM Tris-HCl, 39 mM glycine, 0.0375% SDS) at 22 V for 4 h and blocking overnight in 5% skimmed (low-fat) milk in TBS (10 mM Tris-HCl, pH 7.5, 140 mM NaCl) containing 0.1% Tween-20 (TBS/Tween), the membrane was incubated for 1 h with the appropriate primary antibody. ERK1 was detected using monoclonal antibody against diphosphorylated ERK1/2 (1∶1000, M9692 Sigma) and ERK1 (1∶500, G-8; sc-271269, Santa Cruz Biotechnology) antibodies. Detection of horseradish-peroxidase-coupled secondary antibody was achieved using enhanced chemiluminescence detection (ECL, Amersham Biosciences). All experiments were repeated at least three times.

Gonzalez-Garcia J.R., Bradley J., Nomikos M., Paul L., Machaty Z., Lai F.A, & Swann K. (2014). The dynamics of MAPK inactivation at fertilization in mouse eggs. Journal of Cell Science, 127(12), 2749-2760.

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Piceatannol Inhibits Fibrosis Markers

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Piceatannol was purchased from Future Chem (Seoul, Korea). Anti-alpha smooth muscle actin (α-SMA; 1:1000, sc-130617), anti-CTGF (1:1000, sc-14939), anti-HDAC3 (1:1000, sc-11417), anti-HDAC4 (1:1000, sc-11418), anti-HDAC5 (1:1000, sc-133225), anti-TGF-β1 (1:1000, sc-146), anti-JNK (1:1000, sc-7345), anti-ERK1 (1:1000, sc-271269), and anti-GAPDH (1:1000, sc-32233) antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Primary antibodies against collagen type I (1:1000, ab34710), HDAC2 (1:1000, ab12169), HDAC8 (1:1000, ab137474), and HDAC10 (1:1000, ab53096) were purchased from Abcam (Cambridge, MA, USA). Anti-fibronectin antibody (1:1000, MA5-11981) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Anti-HDAC1 antibody (1:1000, 06–720) was purchased from Merck Millipore (Darmstadt, Germany). Anti-HDAC6 (1:1000, 7612), anti-Smad3 (1:1000, 9523), anti-Smad2 (1:1000, 3103), anti-Smad4 (1:1000, 9515), anti-p-Smad3 (1:1000, 9520), anti-p-JNK (1:1000, 9251), anti-p-p38 (1:1000, 4511), anti-p-ERK1/2 (1:1000, 4370), and anti-p38 (1:1000, 8690) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).

Choi S.Y., Piao Z.H., Jin L., Kim J.H., Kim G.R., Ryu Y., Lin M.Q., Kim H.S., Kee H.J, & Jeong M.H. (2016). Piceatannol Attenuates Renal Fibrosis Induced by Unilateral Ureteral Obstruction via Downregulation of Histone Deacetylase 4/5 or p38-MAPK Signaling. PLoS ONE, 11(11), e0167340.

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