Bp2664100

Manufactured by Thermo Fisher Scientific

The BP2664100 is a laboratory balance from Thermo Fisher Scientific. It is a precision electronic scale designed for weighing applications in laboratory settings. The device provides accurate measurements and has a specified weighing capacity and resolution, but further details about its intended use or performance specifications are not available.

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Lab products found in correlation

G2133, by Merck Group (2 mentions) C3155, by Merck Group (2 mentions) G8270, by Merck Group (1 mentions) Dmi3000 inverted microscope, by Leica camera (1 mentions) C10640, by Thermo Fisher Scientific (1 mentions)

2 protocols using bp2664100

Super-Resolution Imaging Protocol with Oxygen Scavenging

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Prepared cells were imaged in the presence of an SR imaging buffer comprising an oxygen scavenging system (1 mg/mL glucose oxidase [Sigma-Aldrich; G2133], 0.02 mg/mL catalase [Sigma-Aldrich; C3155], and 10% glucose [Sigma-Aldrich; G8270] in phosphate-buffered saline) and 100 mM mercaptoethylamine (Fisher Scientific; BP2664100) (76 (link)). As described previously, all images were acquired on a custom-built SR microscope based on a Leica DMI 3000 inverted microscope (77 (link)) with 200 frames collected at 33 Hz for each color channel. Multicolor channels were corrected using a polynomial morph-type mapping algorithm before and a table of molecular localizations generated using the ImageJ (78 (link)) plugin QuickPALM (79 (link)). Rendered multicolor SR images were analyzed using three complementary approaches as detailed in Fig. 1 C–E and further discussed in SI Appendix, Methods. N values are in SI Appendix, Tables S1 and S2.

Whelan D.R, & Rothenberg E. (2021). Super-resolution mapping of cellular double-strand break resection complexes during homologous recombination. Proceedings of the National Academy of Sciences of the United States of America, 118(11), e2021963118.

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STORM Imaging of Cell Cycle Proteins

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RPP631 cells were incubated in the presence of 100nM YKL-5–124 for 48–72 hr. EdU was pulsed during the last 30 minutes of YKL-5–124 treatment. Treated cells were pre-extracted using 0.5% Triton in CSK buffer (10 mM Hepes, 300 mM Sucrose, 100 mM NaCl, and 3 mM MgCl₂, pH = 7.6) for 10 minutes, and fixed with paraformaldehyde (4%) for 30 minutes. Cells were then rinsed 3 times with PBS and blocked (2% glycine, 2% BSA, 0.2% gelatin, and 50mM NH₄Cl in PBS) overnight at 4 °C for further staining. EdU was tagged with Alexa Fluor 647 picolyl azide through Click reaction (Thermo Fisher, C10640) before immunofluorescent labeling of target proteins, for which the antibodies are mouse anti-PCNA (Santa Cruz Biotech., sc56, 1:1000), goat anti-mouse (Alexa Fluor 750 conjugated, Thermo Fisher, A-21039, 1:5000), and rabbit anti-MCM2 (Alexa Fluor 647 conjugated, Abcam ab223403, 1:500). Fixed cells were mounted onto microscope glass for STORM imaging in freshly mixed imaging buffer (1 mg/mL glucose oxidase (Sigma-Aldrich, G2133), 0.02 mg/mL catalase (Sigma-Aldrich, C3155), 10% glucose (Sigma-Aldrich, G8270), and 100 mM cysteamine (MEA, Fisher Scientific, BP2664100).

Zhang H., Christensen C.L., Dries R., Oser M.G., Deng J., Diskin B., Li F., Pan Y., Zhang X., Yin Y., Papadopoulos E., Pyon V., Thakurdin C., Kwiatkowski N., Jani K., Rabin A.R., Castro D.M., Chen T., Silver H., Huang Q., Bulatovic M., Dowling C.M., Sundberg B., Leggett A., Ranieri M., Han H., Li S., Yang A., Labbe K.E., Almonte C., Sviderskiy V.O., Quinn M., Donaghue J., Wang E.S., Zhang T., He Z., Velcheti V., Hammerman P.S., Freeman G.J., Bonneau R., Kaelin WG J.r., Sutherland K.D., Kersbergen A., Aguirre A.J., Yuan G.C., Rothenberg E., Miller G., Gray N.S, & Wong K.K. (2019). CDK7 Inhibition Potentiates Genome Instability Triggering Anti-Tumor Immunity in Small Cell Lung Cancer. Cancer cell, 37(1), 37-54.e9.

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