Hplc plus

Manufactured by Merck Group

Sourced in Germany, Poland

HPLC Plus is a high-performance liquid chromatography (HPLC) system designed for efficient and reliable separation, identification, and quantification of a wide range of chemical compounds. The system offers advanced features and technologies to support various analytical applications.

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11 protocols using hplc plus

PBX 9501 and BDNPA/F Preparation

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Acetonitrile (HPLC Plus) and dichloroethane (HPLC Plus) were obtained
from Sigma-Aldrich. Ammonium acetate was obtained from VWR and was
diluted to 10 mM using deionized water (HPLC Plus) obtained from Sigma-Aldrich.
The PBX 9501 samples were prepared by dissolving ∼150 mg in
∼3.75 mL of dichloroethane. The solution was then filtered
through a 25 mm diameter Whatman Puradisc 25TF filter (GE Healthcare,
Marlborough, MA) containing a 0.45 μm PTFE membrane in a polypropylene
housing. The BDNPA/F samples [including those from the hydrolysis
experiments (see Section S1.5)] were prepared
by dissolving ∼2 mg in 2 mL of dichloroethane.

Freye C.E, & Snyder C.J. (2022). Investigation into the Decomposition Pathways of an Acetal-Based Plasticizer. ACS Omega, 7(34), 30275-30280.

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PETN Quantification via HPLC

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Acetonitrile (HPLC
Plus) was obtained from Sigma-Aldrich. Ammonium acetate was obtained
from VWR and was diluted to 10 mM using deionized water (HPLC PLUS)
obtained from Sigma-Aldrich. Samples of PETN were dissolved in acetonitrile
at a concentration of ∼0.8 mg/mL. The solution was then filtered
through a 25 mm diameter Whatman Puradisc 25TF filter (GE Healthcare,
Marlborough, MA, USA) containing a 0.45 μm PTFE membrane in
a polypropylene housing.

Manner V.W., Smilowitz L., Freye C.E., Cleveland A.H., Brown G.W., Suvorova N, & Tian H. (2022). Chemical Evaluation and Performance Characterization of Pentaerythritol Tetranitrate (PETN) under Melt Conditions. ACS Materials Au, 2(4), 464-473.

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Potato Dextrose Agar and Solvent Preparation

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Potato dextrose agar (PDA, HIMEDIA India). Hexane Sigma-Aldrich (HPLC Plus, for HPLC, GC, and residue analysis, ≥95%).
Dimethylsulfoxide (DMSO) Sigma-Aldrich (Hybri-Max™, sterile-filtered, BioReagent, suitable for a hybridoma, ≥99.7%).

Tančinová D., Hlebová M., Folitinova D., Mašková Z., & Barboráková Z. (2021). Influence of eight chosen essential oils in the vapor phase on the growth of Rhizopus stolonifer and Rhizopus lyococcus. Potravinarstvo Slovak Journal of Food Sciences, 15, 378-386.

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Intramolecular Radical C-H Amination via Co(II) Catalysis

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Example 8

(Co(II)-Catalyzed Intramolecular Radical 1,5-C—H Amination)

[Figure (not displayed)]

An oven dried Schlenk tube was charged with catalyst (0.002 mmol) or (0.005 mmol) and 4 Å molecular sieves (50 mg). This reaction vessel was evacuated and backfilled with nitrogen several times. The Teflon screw cap was replaced with a rubber septum and the azide substrate (0.1 mmol) was added followed by the addition of 1.0 mL of methyl tert-butyl ether (HPLC plus, residue analysis 99.9% from Aldrich). The Schlenk tube was then purged with nitrogen for 2 minutes and the rubber septum was replaced with a Teflon screw cap. The Schlenk tube was then placed in an oil bath at the indicated temperature while stirring. After the indicated time, the reaction mixture was purified by silica gel column chromatography (Conditions were given previously). The fractions containing product were collected and concentrated by rotary evaporation to obtain the target compound. All the racemic products (for HPLC) were obtained following the same procedure with achiral catalyst [Co(P9)][Co(3,5-Di^tBu-IbuPhyrin)].

US11161827B2. Catalytic systems for stereoselective synthesis of chiral amines by enantiodivergent radical C—H amination (2021-11-02). Trustees of Boston College [US]. Inventors: Xiao-Xiang Zhang [US], Kai Lang [US].

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Synthesis of Functionalized Polymeric Materials

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Ethyl hexyl methacrylate (EHMA, 2-ethylhexyl-2-methylprop-2-enoate: TCI, >99.0%), 1,6 hexanediol dimethacrylate (HDDMA, 6-(2-methylprop-2-enoyloxy)hexyl 2-methylprop-2-enoate: Aldrich, ≥90%), styrene (Aldrich, 99.9%) and divinylbenzene (DVB, Aldrich, 90%, 80 para content, technical mixture) were passed through an alumina column to remove inhibitors prior to use. Dodecyl(trimethyl)ammonium bromide (DTMAB, 1-dodecyl (trimethyl)azanium; bromide: TCI > 98%), potassium persulfate (KPS, >99% Acros Organics), tetrahydrofuran (THF, Sigma-Aldrich, >99%), methanol (Sigma-Aldrich, >99%), and deionized water (DI water, Sigma-Aldrich, HPLC Plus) were used as received.

Fischer J., Han L., Saito T, & Dadmun M. (2022). When does a macromolecule transition from a polymer chain to a nanoparticle?. Nanoscale Advances, 4(23), 5164-5177.

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MALDI-TOF MS Analysis of VuEI Peptide

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The sample containing VuEI (16 pmol) was spotted onto a MSP 96 polished steel target plate (Bruker Daltonics, Germany) and air-dried at room temperature. The spots were covered with 2 μL of saturated matrix solution (alpha-cyano-4-hydroxycinnamic acid, Bruker Daltonics, Germany) prepared in 50% acetonitrile HPLC Plus (Sigma Aldrich) and 0.1% trifluoroacetic acid (Pierce Biotechnology, USA). The mass spectra were acquired using MALDI-TOF MS spectrometer in a linear positive mode (Microflex, Bruker Daltonics, Germany). Bacterial test standard (BTS, Bruker) was used for instrument calibration. The mass spectra data was analyzed in a range of 2,000 to 20,000 m/z.

Ferreira G.C., Duran A.F., da Silva F.R., Bomediano L.D., Machado G.C, & Sasaki S.D. (2019). Neutrophil elastase inhibitor purification strategy from cowpea seeds. PLoS ONE, 14(10), e0223713.

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Protein Sample Preparation for LC-MS

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Eighty micrograms of each sample were reduced with 10 mM dithiothreitol (BioUltra, Sigma) for 45 min at 56 °C, alkylated with 20 mM iodoacetamide (BioUltra, Sigma) for 30 min at room temperature in the dark, and then precipitated with acetone (HPLC Plus, Sigma) overnight at −20 °C. The dried sample was resuspended in 50 mM ammonium bicarbonate (BioUltra, Sigma) and digested overnight at 37 °C with trypsin (Sequencing Grade Modified Trypsin, Promega) in a 1:50 trypsin:sample ratio. A second digestion was performed for 3 h at 37 °C, by adding trypsin in a 1:100 trypsin:sample ratio with 80% (v/v) acetonitrile (Optima LC/MS grade, Fisher Scientific). The sample was dried on a SpeedVac (ThermoSavant Scientific) and resuspended in 5% Formic acid (Optima LC/MS grade, Fisher Scientific) to perform peptide cleanup using C18 microcolumns (OMIX C18 pipette tips, Agilent), and then dried again.

Chegão A., Guarda M., Alexandre B.M., Shvachiy L., Temido-Ferreira M., Marques-Morgado I., Fernandes Gomes B., Matthiesen R., Lopes L.V., Florindo P.R., Gomes R.A., Gomes-Alves P., Coelho J.E., Outeiro T.F, & Vicente Miranda H. (2022). Glycation modulates glutamatergic signaling and exacerbates Parkinson’s disease-like phenotypes. NPJ Parkinson's Disease, 8, 51.

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DPPH Radical Scavenging Assay of C. asiatica

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The 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) and methanol (HPLC Plus, ≥99.9%) were purchased from Sigma Aldrich.
The ability of samples to scavenge DPPH radicals was measured adding 40 μl of the C. asiatica extract contained in each nanocomposite to 960 μl of DPPH methanolic solutions. DPPH methanolic solution at the same dilution was also used as control. Absorbance was measured at 517 nm after storage at room temperature for 30 min in the dark, using a UV instrument Perkin Elmer mod. Lambda 25 (Monza, Italy). Experiments were performed in triplicate. The free radical scavenging activity, expressed as percentage of antioxidant activity (AA%), was calculated according to the following formula²⁷:

AA % = [(A_{DPPH} - A_{sample}) / A_{DPPH}] * 100,

where A is the absorbance.
The obtained results were compared with the activity of the pure C. asiatica glycolic extract at the same concentration.

Ebau F., Scano A., Manca M.L., Manconi M., Cabras V., Pilloni M, & Ennas G. (2022). Centella asiatica extract‐SiO2 nanocomposite: More than a drug‐delivery system for skin protection from oxidative damage. Journal of Biomedical Materials Research. Part a, 111(3), 300-308.

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Purification of Chemicals for Experiments

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All the chemicals were purchased from Merck-Sigma-Aldrich in the commercially available highest purity grade and used without further purification. HPLC-grade ultrapure water (HPLC Plus, Sigma-Aldrich) was used for all the experimental steps.

Nocito G., Zribi R., Chelly M., Pulvirenti L., Nicotra G., Bongiorno C., Arrigo A., Fazio B., Neri G., Nastasi F, & Conoci S. (2024). Photochemical synthesis, characterization, and electrochemical sensing properties of CD-AuNP nanohybrids. Nanoscale, 16(7).

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Photoinitiated PISA Synthesis of Polymers

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At first, 63.44 uL of hydroxypropyl methacrylate, 13.9 mg (6.95 μmol) of Poly(ethylene glycol) methyl ether 4-cyano-4-(phenylcarbonothioylthio) or m-RAFT, 3.26 uL of Ru(bpy)₃Cl₂ (8.5 mM), and 2 mL water (HPLC Plus, Sigma-Aldrich) were added to a 1 dram glass vial. The mixture was then vortexed for 5 min followed by nitrogen bubbling for 15 min (5 min over the headspace of the liquid and 10 min within the liquid). The mixture was transferred to a 1.5 mL quartz cuvette (purged with nitrogen prior to transfer) which was then capped with a Teflon stopper. The cuvette was then irradiated for 16 h using 7 blue LED units (6.57 mW for each LED unit) and the temperature was maintained at 25 °C using circulation of water around the sample with a pump connected to water-bath. For the other PISA reactions using different monomers such as butyl acrylate, hydroxybutyl acrylate, and polystyrene, equal number of moles of the monomer was used to replace hydroxypropyl methacrylate in the mixture followed by the same reaction process.

Lin C., Katla S.K, & Pérez-Mercader J. (2021). Photochemically induced cyclic morphological dynamics via degradation of autonomously produced, self-assembled polymer vesicles. Communications Chemistry, 4, 25.

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