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Ab277390

Manufactured by Abcam
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Ab277390 is a monoclonal antibody that targets the human CD4 protein. It is designed for use in a variety of experimental techniques, including flow cytometry and immunohistochemistry. The core function of this product is to specifically bind to the CD4 antigen, which is expressed on the surface of T helper cells.

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Lab products found in correlation

Mak266, by Merck Group (2 mentions) Mak052, by Merck Group (2 mentions) Mak055, by Merck Group (2 mentions) Ab65359, by Abcam (2 mentions) Ab83369, by Abcam (2 mentions) Ke10048, by Proteintech (1 mentions) Ab208348, by Abcam (1 mentions) Fully automatic biochemical analyzer, by Hitachi (1 mentions) Ab222503, by Abcam (1 mentions) Ab226900, by Abcam (1 mentions) Ab199082, by Abcam (1 mentions) Glucometer, by Roche (1 mentions) Ab100718, by Abcam (1 mentions) Ab108785, by Abcam (1 mentions) Ab65390, by Abcam (1 mentions) Ab65336, by Abcam (1 mentions)

11 protocols using ab277390

1

Metabolic Markers After BPA Exposure

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At 8 weeks or 16 weeks after BPA exposure, we determined the fasting glucose (FG) and fasting insulin (FI) levels. After an overnight fast with ad libitum access to water, mouse tail venous blood was collected. Blood glucose was determined using a glucometer, and serum insulin was measured with an ELISA kit (ab277390, Abcam). FG and FI were used to calculate HOMA-IR, with HOMA-IR = FG (mmol/L)∗FI (μU/mL)/22.5.
Wang G., Hong X., Yu J., Zhang Y., Li Y., Li Z., Zhu Z., Yuan S., Zhang X., Wang S., Zhu F., Wang Y., Wu C., Su P, & Shen T. (2023). Enhancing de novo ceramide synthesis induced by bisphenol A exposure aggravates metabolic derangement during obesity. Molecular Metabolism, 73, 101741.
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2

Diabetes induction in C57/BL6 mice

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C57/BL6 mice at 6 weeks of age were given 5-day low dose of STZ (572201-1GM, Millipore Sigma) IP 25mg/kg(18 , 19 (link)). DM developed for 12 weeks. We performed body weight, 16hr fasting glycemia (75840-798, VWR) and insulin quantification via ELISA (ab277390, Abcam) in all mice.
Mota Alvidrez R.I., Annarapu G.K., Srinivasan A.J., Liu Z., Yazdani H.O., Simmons R.L., Shiva S., Neal M.D, & Nolfi-Donegan D. (2023). High Dose of Metformin Decreases Susceptibility to Occlusive Arterial Thrombosis in Diabetic Mice. Research Square.
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3

Fasting Serum Insulin Quantification

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Mouse Insulin ELISA (Abcam: ab277390) kit was used to measure insulin level in serum samples from fasted animals at the end of the experiment.
Ali D., Figeac F., Caci A., Ditzel N., Schmal C., Kerckhofs G., Havelund J., Færgeman N., Rauch A., Tencerova M, & Kassem M. (2022). High‐fat diet‐induced obesity augments the deleterious effects of estrogen deficiency on bone: Evidence from ovariectomized mice. Aging Cell, 21(12), e13726.
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4

Orbital Blood Collection for Metabolic Markers

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The experimental mice were anesthetized, and the skin around eye was pulled away to allow the eye to bulge out of the socket. Then a sterile capillary tube was penetrated into the corner of the eye socket underneath the eyeball to collect blood from orbital sinus into an Eppendorf tube. The samples were centrifuged (4°C, 3000 rpm, 15 min), then the supernatants were collected into Eppendorf tubes and stored at -80°C until use. The concentrations of leptin, insulin and triglycerides were detected by using the ELISA kits (leptin: KE10048, Proteintech; Insulin: ab277390, Abcam; Triglyceride: MAK266, Sigma-Aldrich.), according to the manufacturer’s instructions.
Wang G., Li S., Li Y., Zhang M., Xu T., Li T., Cao L, & Lu J. (2023). Corticosterone induces obesity partly via promoting intestinal cell proliferation and survival. Frontiers in Endocrinology, 13, 1052487.
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5

Metabolic Biomarkers in Disease Evaluation

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The levels of blood glucose (Glu), low-density lipoprotein (LDL), high-density lipoprotein (HDL), total cholesterol (TC), and triglyceride (TG) were determined using a fully automatic biochemical analyzer (Hitachi, Tokyo, Japan). In addition, insulin (Ins), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) contents were determined using ELISA kits (ab277390, ab222503 and ab208348, Abcam, Cambridge, MA, USA). Lipopolysaccharide (LPS) content was determined using commercial kits (JYM0588Mo, Jiyinmei Biotechnology Co., Ltd., Wuhan, China) following specific protocols. Homeostasis model assessment (HOMA)-insulin resistance (IR) were calculated according to the following formula:
Wang K., Wang Y., Chen S., Gu J, & Ni Y. (2022). Insoluble and Soluble Dietary Fibers from Kiwifruit (Actinidia deliciosa) Modify Gut Microbiota to Alleviate High-Fat Diet and Streptozotocin-Induced TYPE 2 Diabetes in Rats. Nutrients, 14(16), 3369.
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6

Metabolic Biomarker Profiling in Biological Samples

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The concentration of serum alanine transaminase (ALT) (#MAK052, Sigma-Aldrich), aspartate aminotransferase (AST) (#MAK055, Sigma-Aldrich), alkaline phosphatase (AKP) (#ab83369, Abcam), serum insulin (#ab277390, Abcam) and hepatic triglyceride (TG) (#MAK266, Sigma-Aldrich), total cholesterol (TC) (#ab65359, Abcam) and non-esterified free fatty acids (NEFA) (#E-BC-K014, Elabscience, Inc., Houston, USA) were detected using commercially-available detection kits in the indicated groups according to the product specification.
Xu M., Tan J., Dong W., Zou B., Teng X., Zhu L., Ge C., Dai X., Kuang Q., Zhong S., Lai L., Yi C., Tang T., Zhao J., Wang L., Liu J., Wei H., Sun Y., Yang Q., Li Q., Lou D., Hu L., Liu X., Kuang G., Luo J., Xiong M., Feng J., Zhang C, & Wang B. (2022). The E3 ubiquitin-protein ligase Trim31 alleviates non-alcoholic fatty liver disease by targeting Rhbdf2 in mouse hepatocytes. Nature Communications, 13, 1052.
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7

Streptozotocin-Induced Diabetes Model

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C57/BL6 mice at 6 weeks of age were given 5-day low dose of STZ (572201-1GM, Millipore Sigma) Intraperitoneal (IP) 25mg/kg(18 , 19 (link)) for DM mice model development. DM developed for 12 weeks. Non-DM (healthy) control mice were injected with saline IP for 5 days with the same amount of volume that Streptozotocin treated DM mice received. We performed routine mouse assessment as well as body weight and 16hr fasting glycemia (75840-798, VWR) and insulin quantification via ELISA (ab277390, Abcam) in all mice as a standard to corroborate the correct model development which is well described in the literature as a DM model.
Lu S.S, & Yau S.T. (1990). Holomorphic curves in surfaces of general type.. Proceedings of the National Academy of Sciences of the United States of America, 87(1), 80-82.
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8

Quantification of Adipokines and Insulin

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Leptin, adiponectin, and insulin were quantified using specific ELISA Kits (cat. ab199082, ab226900, ab277390, respectively; Abcam, Cambridge, UK).
Kim H.R., Seo E., Oh S., Seo M., Byun K, & Kim B.Y. (2022). Anti-Obesity Effects of Multi-Strain Probiotics in Mice with High-Carbohydrate Diet-Induced Obesity and the Underlying Molecular Mechanisms. Nutrients, 14(23), 5173.
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9

Metabolic Biomarker Evaluation

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The concentration of serum alanine transaminase (ALT) (#MAK052, Sigma–Aldrich), aspartate aminotransferase (AST) (#MAK055, Sigma–Aldrich), alkaline phosphatase (AKP) (#ab83369, Abcam), serum insulin (#ab277390, Abcam), and hepatic triglyceride (TG) (#MAK266, Sigma–Aldrich), total cholesterol (TC) (#ab65359, Abcam) and nonesterified free fatty acids (NEFA) (#E-BC-K014, Elabscience, Inc., Houston, USA) were detected using commercially available detection kits according to the manufacturer s’ protocols.
Ge C., Tan J., Dai X., Kuang Q., Zhong S., Lai L., Yi C., Sun Y., Luo J., Zhang C., Zhu L., Wang B, & Xu M. (2022). Hepatocyte phosphatase DUSP22 mitigates NASH-HCC progression by targeting FAK. Nature Communications, 13, 5945.
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10

Metabolic Profiling of Fasted Mice

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Mice were fasted overnight (12 h), and tail vein blood was collected. Plasma samples were stored at −20 °C until use. Concentrations of insulin (Abcam, ab277390), leptin (Abcam, ab100718), and adiponectin (Abcam, ab108785) were measured using ELISA kits. Concentrations of total cholesterol, triglyceride, and free fatty acid were measured using assay kits (Wako Diagnostics). For glucose tolerance test, mice were fasted overnight and then treated with an intraperitoneal injection of 0.75 g/kg glucose, followed by measurement of blood glucose levels with a glucometer (Roche). To assess insulin tolerance, mice were fasted for 4 h before receiving an intraperitoneal injection of insulin (1.5 units/kg body weight) and were then subjected to measurement of blood glucose levels.
Song Y., Zan W., Qin L., Han S., Ye L., Wang M., Jiang B., Fang P., Liu Q., Shao C., Gong Y, & Li P. (2021). Ablation of ORMDL3 impairs adipose tissue thermogenesis and insulin sensitivity by increasing ceramide generation. Molecular Metabolism, 56, 101423.
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