Avance dpx 300 ft nmr spectrometer

Optical rotations were obtained with a Perkin-Elmer 341 polarimeter (Boston, MA, USA). UV spectra were measured on a Milton Roy Spectronic 300 Array spectrophotometer, and IR was recorded on a Perkin-Elmer FT-IR 1760x spectrophotometer (Boston, MA, USA). High-resolution electrospray ionization mass spectra (HR-ESI-MS) were recorded with a Bruker micro TOF mass spectrometer (Billerica, MA, USA). NMR spectra were obtained with a Bruker Avance DPX-300 FT-NMR spectrometer (Billerica, MA, USA). Vacuum liquid chromatography (VLC) and column chromatography (CC) were performed on silica gel 60 (Merck, 70–230 µm, Darmstadt, Germany), silica gel 60 (Merck, 230–400 nm) or Sephadex LH-20 (Pharmacia, Piscataway, NJ, USA). Yeast α-glucosidase enzyme, p-nitrophenol-α-d-glucopyranoside, pancreatic lipase, and 4-methylumbelliferyl oleate were purchased from Sigma Chemical, Inc. (St. Louis, MO, USA), and acarbose from Fluka Chemical (Buchs, Switzerland).

Optical rotations were determined with a PerkinElmer Polarimeter 341 (Boston, MA, USA). UV spectra were recorded on a Milton Roy Spectronic 3000 Array spectrophotometer (Rochester, Monroe, NY, USA). IR spectra were obtained with a PerkinElmer FT-IR 1760X spectrophotometer (Boston, MA, USA). Mass spectra were measured using a Bruker MicroTOF mass spectrometer (ESI-MS) (Billerica, MA, USA). NMR spectra were recorded on a Bruker Avance DPX-300FT NMR spectrometer or a Bruker Avance III HD 500 NMR spectrometer (Billerica, MA, USA). Yeast α-glucosidase enzyme and p-nitrophenol-α-d-glucopyranoside were obtained from Sigma Chemical, Inc. (St. Louis, MO, USA), and acarbose was purchased from Fluka Chemical (Buchs, Switzerland). Microtiter plate readings were carried out with a CLARIOstar apparatus (BMGLABTECH, Ortenberg, Germany).

The Milton Roy Spectronic 3000 Array spectrophotometer (Rochester, Monroe, NY, USA) was used to record UV spectra, while IR spectra were obtained from the PerkinElmer FT-IR 1760X spectrophotometer (Boston, MA, USA). Mass spectra were determined using the Bruker MicroTOF mass spectrometer (ESI-MS) (Billerica, MA, USA). NMR spectra were recorded by the Bruker Avance DPX-300FT NMR spectrometer or the Bruker Avance III HD 500 NMR spectrometer (Billerica, MA, USA). Sigma Aldrich (Sigma-Aldrich, Dorset, UK) provided 2′,7′-dichlorofluorescein di-acetate (DCFH-DA), and Merck Millipore (Merck Millipore, Darmstadt, Germany) supplied fetal bovine serum (FBS). Invitrogen (Invitrogen Ltd., Paisley, UK) provided Dulbecco’s modified Eagle’s Medium/Nutrient Mixture F-12 Ham (DMEM/F-12), penicillin–streptomycin, H₂O₂, dimethyl sulfoxide (DMSO), and 3-[4,5-dimethyltiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT). Antibodies used in western blotting were obtained from Cell Signaling Technology (Danvers, MA, USA). Antioxidant enzyme assay test kits were obtained from Cayman Chemical (Cayman Chemical, Ann Arbor, MI, USA).