Csu x1 m1 spinning disk

Spinning disk images were acquired at 37°C in M2 + BSA + 1 µM milrinone using a Plan-APO 40×/1.25 NA objective on a Leica DMI6000B microscope enclosed in a thermostatic chamber (Life Imaging Service) equipped with a CoolSnap HQ2/CCD-camera (Princeton Instruments) coupled to a Sutter filter wheel (Roper Scientific) and a Yokogawa CSU-X1-M1 spinning disk. Oil droplet images were acquired using either the stream mode of the camera on Metamorph (one image every 500 ms) or one image every 20 min to follow the whole motion toward the oocyte center. The actin cytoplasmic meshwork decorated with GFP-UtrCH was imaged using Metamorph upon excitation at 491 nm.

Spinning disk images of live oocytes in M2+BSA medium were acquired at 37 °C using a Plan-APO 40×/1.25 NA objective on a Leica DMI6000B microscope enclosed in a thermostatic chamber (Life Imaging Service, Basel, Switzerland) equipped with a CoolSnap HQ2/CCD-camera (Princeton Instruments) coupled to a Sutter filter wheel (Roper Scientific, Munich, Germany) and a Yokogawa CSU-X1-M1 spinning disk. The Metamorph software (Universal Imaging, Bedford Hills, New York, USA) was used for data collection. For live chromosome imaging, seven z-planes were acquired, spaced 3 μm, every 30 min starting 0530 h after nuclear envelope breakdown, with a power of 10% of laser −581 nm and 200 ms acquisition time.

Spinning disk movies were acquired using a Plan‐APO 40×/1.25NA objective on a Leica DMI6000B microscope enclosed in a thermostatic chamber (Life Imaging Service) equipped with a CoolSnap HQ2/CCD camera coupled to a Sutter filter wheel (Roper Scientific) and a Yokogawa CSU‐X1‐M1 spinning disk. Metamorph Software (Universal Imaging) was used to collect data.
For SIM super‐resolution microscopy of aMTOCs, image acquisition was performed in 3D SIM mode, with a N‐SIM Nikon microscope (Nikon Imaging Centre @ Institut Curie‐CNRS) before image reconstruction using the NISElements software 48. The system is equipped with an APO TIRF 100× 1.49NA Oil Immersion, a laser illumination (488 nm at 200 mW and 561 nm at 100 mW), and an EMCCD DU‐897 Andor camera.