Blood from 30- and 90-week-old RPE and wild-type mice (n = 3/group) was collected from the vein tail into lithium-heparin Microvette® 200 (Sarstedt). Samples were processed with the Sysmex XP-300™ Automated Hematology Analyzer (Sysmex Corporation, Japan) or treated with Red Blood Cell lysis solution (Qiagen). Thereafter, white blood cells were manually counted using a haemocytometer.
Microvette 200
Manufactured by Sarstedt
Sourced in United States
The Microvette® 200 is a compact, single-use blood collection device with a capacity of 200 μl. It is designed for the collection and storage of small blood samples.
Automatically generated - may contain errors
3 protocols using microvette 200
1
Comparative Hematological Analysis in Mice
2
Biochemical Profile of Venous Blood
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The vein blood (ca 0.3 mL) was collected in blood collection serum tubes coated with a clot activator on the inner wall of the tubes (Microvette® 200 Sarstedt, Marfour, Polska, cat. no. 20.1292). The blood test was carried out up to three hours after blood taking. For the biochemical profile, we used the Olympus AU680 Clinical Chemistry Analyzer and calorimetric method at 37 °C. We checked the following parameters and methods: enzymes (AST (aspartate aminotransferase), ALT (alanine aminotransferase)—IFCC Reference method with Pyrioxal Phosphate; Ap—IFCC Reference Method 2011), albumin—Bromocresol Green, urea—Urease/GLDH, creatinine—Jaffe IDMS and c-reactive protein.
3
Blood Collection and Plasma Preservation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood was collected immediately after euthanization with CO2 and transferred into Li-heparin tubes (Microvette 200; Sarstedt, Newton, NC, USA). Tubes were centrifuged at 2,000 × g for 10 min. Plasma was transferred into an Eppendorf tube, snap-frozen in liquid nitrogen, and kept at −80°C. Plasma (minimum 50 μL) biochemical analyses were done by the Division of Clinical Chemistry and Biochemistry at the University Children’s Hospital Zürich (Zürich, Switzerland) with a commercial routine method on an Alinity c (Abbot Laboratories, Chicago, IL, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!