For quantitative analysis, the samples from the SDR (pretreated and untreated) and enzymatic saccharification samples obtained were filtered from a membrane filter of 0.22 µm. The samples were analyzed quantitatively for glucose, xylose, arabinose, cellobiose, and other organic acids such as acetic acid, formic acid, and lactic acid using HPLC (Agilent Technology 1260 series instrument), with a refractive index detector at 35 °C (Agilent Technology 1260 series instrument), Metacarb 87 H column with 60 °C temperature, and 0.005 M sulphuric acid mobile phase with a flow rate of 0.65 mL/min [19 (link)] was used.
1260 series instrument
Manufactured by Agilent Technologies
Sourced in United States
The 1260 series instrument from Agilent Technologies is a versatile lab equipment designed for various analytical applications. It offers precise and reliable performance, providing core functionality for the intended use. The detailed technical specifications and capabilities of this product are not available within the scope of this response.
Automatically generated - may contain errors
Lab products found in correlation
Silica gel, by Qingdao Haiyang Chemical (3 mentions)
Uv 3600 spectrophotometer, by Hitachi (1 mentions)
F 7000 spectrophotometer, by Hitachi (1 mentions)
Eclipse xdb c18, by Agilent Technologies (1 mentions)
Eclipse plus c8 column, by Agilent Technologies (1 mentions)
Zorbax eclipse xdb c18 column, by Agilent Technologies (1 mentions)
6529b q tof mass instrument, by Agilent Technologies (1 mentions)
Rp c18 column, by Agilent Technologies (1 mentions)
Aviii 500 nmr instrument, by Bruker (1 mentions)
P 1020 polarimeter, by Jasco (1 mentions)
Tensor 27 spectrometer, by Bruker (1 mentions)
Lh 20, by Mitsubishi (1 mentions)
Eclipse c18 column, by Agilent Technologies (1 mentions)
5800 maldi tof mass spectrometer, by AB Sciex (1 mentions)
10 protocols using 1260 series instrument
1
Quantitative Analysis of Lignocellulosic Biomass
2
HPLC Analysis of TA Drug Purity
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Drug purity and percentage free drug were measured using a proprietary validated reversed-phase gradient HPLC method. The chromatographic equipment used was an Agilent 1260 series instrument with diode array UV detection. The HPLC instrument was fitted with a functionalized mixed-mode C18 column (150 mm × 4.6 mm, particle size 3 µm). The injection volume was 20 µL.
Standard solutions of TA (USP, Rockville, Maryland, USA) were prepared in dimethyl sulfoxide (DMSO) with subsequent dilution in a water/acetonitrile/formic acid HPLC diluent to achieve a final TA concentration of 0.2 mg/mL.
Samples were prepared by harvesting drug product particles using centrifuge filtration. The filtrate was collected and dried at 30 °C for 1 h, with further drying at 25 °C overnight as necessary. Once dried, sample particles were dissolved in DMSO with subsequent dilution in HPLC diluent to a final TA concentration of 0.2 mg/mL. Impurities were identified on the basis of the retention time of known compounds and quantified as percentage weight using validated response factors. The percentage free drug in each TA-ER ± local anesthetic preparation was assessed by analyzing the supernatant from the centrifugation step of the impurity sample preparation.
Standard solutions of TA (USP, Rockville, Maryland, USA) were prepared in dimethyl sulfoxide (DMSO) with subsequent dilution in a water/acetonitrile/formic acid HPLC diluent to achieve a final TA concentration of 0.2 mg/mL.
Samples were prepared by harvesting drug product particles using centrifuge filtration. The filtrate was collected and dried at 30 °C for 1 h, with further drying at 25 °C overnight as necessary. Once dried, sample particles were dissolved in DMSO with subsequent dilution in HPLC diluent to a final TA concentration of 0.2 mg/mL. Impurities were identified on the basis of the retention time of known compounds and quantified as percentage weight using validated response factors. The percentage free drug in each TA-ER ± local anesthetic preparation was assessed by analyzing the supernatant from the centrifugation step of the impurity sample preparation.
3
Characterization of Phosphorescent OLED Materials
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1H and 13C NMR spectra were recorded on a Bruker Varian and Ascend 400 MHz spectrometer in CDCl3. UV–Vis absorption spectra were recorded on a Shimadzu UV-3600 spectrophotometer, and photoluminescence spectra were measured on a Hitachi F-7000 spectrophotometer. Cyclic voltammetry (CV) measurements were carried out with a CHI 600D system in acetonitrile solution containing 0.1 M tetrabutylammonium tetrafluoroborate (TBABF4) as the supporting electrolyte, Ag/AgNO3 as the reference electrode, a platinum wire as the counter electrode, and a platinum working electrode. The current density–voltage–luminance (I–V–L) characteristics were measured, and the electroluminescence (EL) spectra of the phosphorescent OLEDs were recorded using a Keithley 2400 source measurement unit and a CS-1000 spectrophotometer. A Keithley 2400 source measurement unit and a CS-1000 spectroradiometer were used to evaluate the device performance. The molecular weight was measured by gel permeation chromatography (GPC) with polystyrene standard and THF as the eluent using an Agilent 1260 Series instrument. The surface morphologies were measured by atomic force microscopy (AFM; Park Systems NX-20) in the non-contact scanning mode.
4
Scaled-up Compound Purification and NMR Structural Elucidation
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To obtain sufficient compound for structural elucidation by NMR, cultures were scaled up to 500 mL batches to a total volume of 5 Liters and expressed using the combinatorial expression system described above (Kitaoka et al., 2015 (link)). The resultant products were extracted with an equal volume of hexanes. The organic extract was recovered using a separatory funnel and then dried by rotary evaporation. The resulting residue was passed through a silica column and eluted using a hexane: ethyl acetate gradient. Fractions of interest were further purified by HPLC using an Agilent 1260 series instrument equipped with an autosampler, fraction collector and diode array UV detector over a ZORBAX Eclipse XDB C18 (4.6 x 150 mm, 5 mm) at a 0.5 mL/min flow rate using a water and acetonitrile gradient as the mobile phase. Purified compounds were resuspended in CDCl3. Structural analysis was performed using 1D (‘1H, 13C’) and 2D (TOCSY, ROESYPHPR, HMBCGP, DQFCOSY, and 13CHSQC) NMR experiments. Spectra were acquired on a Bruker Avance 500-MHz TopSpin NMR spectrometer.
5
Phytochemical Profiling of Calotropis procera Leaves
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The phytochemical compositions of the extract from leaves of Calotropis procera were determined using the HPLC UV–Vis Detectors to find phenolic, flavonoids, and other active compounds, and analysis was performed with an Agilent 1260 series instrument. The analysis was conducted in the Central Labs Unit of the Research National Center, Egypt. Using a Zorbax Eclipse Plus C8 column (4.6 mm × 250 mm i.d., 5 μm), the separation was performed. At a flow rate of 0.9 mL/min, the mobile phase was composed of water (A) and 0.05% trifluoroacetic acid in acetonitrile (B). The following was the sequential linear gradient programming for the mobile phase: 0 min (82% A), 0–1 min (82% A), 1–11 min (75% A), 11–18 min (60% A), 18–22 min (82% A), and 22–24 min (82% A). At 280 nm, the multi-wavelength detector was observed. For every sample solution, there was one injection volume of five microliters. At 40 °C, the column temperature was kept constant.
6
Pharmacokinetic and CYP Metabolic Studies
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Pharmacokinetic studies in vivo37 (link) and CYP-associated metabolic studies in vitro56 (link) were performed using the method reported previously in our laboratory. In this study, the effects of AIL on CYP activities were investigated using rat and human liver microsomes, employing phenacetin (CYP1A2), tolbutamide (CYP2C9/11), dextromethorphan (CYP2D1/6), chlorzoxazone (CYP2E1) and testosterone (CYP3A2/4) as the probe substrates. They were analysed on an Agilent 1260 series instrument with DAD detection and separated by an Agilent ZORBAX Eclipse XDB-C18 column (4.6 × 150 mm, 5 μm) with a guard column in the respective gradient elution procedure. The incubation system, sample preparation and chromatography conditions are as described previously56 (link).
7
Spectroscopic Characterization of Compounds
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The optical rotations were measured on a JASCO P-1020 polarimeter at room temperature. The melting points were measured using an X-4 digital display micromelting apparatus and are uncorrected. The IR spectra were recorded on a Bruker Tensor 27 spectrometer using KBr pellets. The 1D- and 2D-NMR spectra were measured on a Bruker AVIII-500 NMR instrument (1H: 500 MHz, 13C: 125 MHz) using TMS as the internal standards. HRESIMS was performed on an Agilent 6529B Q-TOF mass instrument using electrospray ionization. All of the solvents used were of analytical grade (Jiangsu Hanbang Science and Technology. Co., Ltd.). Silica gel (200–300 mesh, Qingdao Haiyang Chemical Co., Ltd, China), Sephadex LH-20 (Pharmacia, Sweden), MCI (Mitsubishi, Japan) and RP-C18 silica (40–63 μm, Fuji, Japan) were used for the column chromatography. Preparative HPLC was conducted using an Agilent 1260 Series instrument with a Shim-Pak RP-C18 column (20 × 200 mm), at a flow rate of 10.0 mL/min, and detection by a binary channel UV detector. The fractions obtained from CC were monitored by TLC with precoated Silica gel GF254 (Qingdao Haiyang Chemical Co., Ltd, China) plates.
8
HPLC Analysis of Extracellular Vesicles
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HPLC analysis of the EV is performed following [57 (link)] with some modifications. The SCRE was analyzed using an Agilent 1260 series instrument and Eclipse C18 column (4.6 mm × 250 mm i.d., 5 µm). Separation was performed at a flow rate of 0.9 mL/min. The mobile phase consisted of water (reservoir A) and 0.5% trifluoroacetic acid in acetonitrile (reservoir B) at a concentration of 0.1%. The mobile phase was sequentially programmed with a linear gradient as follows: 0 min (82%A); 0–5 min (80%A); 5–8 min (60%A); 8–12 min (60%A); 12–15 min (82%A); 15–16 min (82%A); and 16–20 (82%A). A multi-wavelength UV detector was used for detection at 280 nm. The injection volume for each sample solution was 5 μL. The column temperature was retained at 40 °C.
9
HPLC Analysis of Organic Compounds
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Agilent 1260 series instrument;
Supelcosil SPLC-18 column (25 cm
x 44 mm, 5 μm)). The following gradient was used with (A) water,
(B) 5 mM ammonium formate, and (C) methanol: 0 min, 100% B; 7 min,
10% A, 90% B; 12 min, 25% A/60% B/15% C; 17 min, 25% A/10% B/65% C;
19 min to 29 min, 100% B.
Supelcosil SPLC-18 column (25 cm
x 44 mm, 5 μm)). The following gradient was used with (A) water,
(B) 5 mM ammonium formate, and (C) methanol: 0 min, 100% B; 7 min,
10% A, 90% B; 12 min, 25% A/60% B/15% C; 17 min, 25% A/10% B/65% C;
19 min to 29 min, 100% B.
10
Isolation and Identification of Antifungal Lipopeptides
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The antifungal substances contained in the crude extract were isolated by HPLC using a 1260 series instrument (Agilent, Santa Clara, CA, United States) equipped with a C18 reversed-phase column (150 mm × 4.6 mm, 5 μm). After consulting the literature and exploring the separation conditions, the optimum separation conditions were determined as follows: The mobile phase comprised (A) 0.1% trifluoroacetic acid in water and (B) acetonitrile (60:40), a flow rate of 0.8 ml/min, the column temperature was 25°C, and a UV detector was employed at a detection wavelength of 230 nm. The eluent of each peak was collected in an Eppendorf tube and the corresponding retention time was recorded. The eluents were vacuum dried and dissolved in sterile water for subsequent activity detection. Eluents possessing antifungal activity against Ggt were analyzed using a 5800 MALDI-TOF mass spectrometer (AB SCIEX, Redwood, WA, United States) employed in positive reflectron mode with a matrix comprising α-cyano-4-hydroxycinnamic acid (Velho et al., 2011 (link)). Isolated and identified lipopeptides from the culture supernatant of strain Z-14 were assessed by HPLC to determine their purity using the parameter settings described above.
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