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Coulter cytomics fc500

Manufactured by Beckman Coulter
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The Coulter Cytomics FC500 is a flow cytometer designed for multi-parameter analysis of cells and particles. The instrument utilizes laser-based technology to detect and analyze various physical and fluorescent properties of individual cells or particles suspended in a fluid stream.

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Lab products found in correlation

Annexin 5 fluos, by Roche (8 mentions) Stem kit, by Beckman Coulter (1 mentions) Xl system 2 software, by Beckman Coulter (1 mentions) Annexin 5 fluos kit, by Roche (1 mentions)

Spelling variants of this product

Cytomics FC500 (870 citations) FC500 (703 citations) Cytomic FC500 (26 citations) Coulter FC500 (8 citations) Coulter Cytomics FC500 Flow Cytometer (3 citations)

11 protocols using coulter cytomics fc500

1

Quantification of Circulating CD34+ Cells

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After 12-h of fasting, 3 mL of heparinized peripheral blood were obtained and CD34+ cells were measured. The precise number of circulating CD34+ cells was quantified as we described previously7 (link). We evaluated circulating CD34+ cells with a Stem-Kit™ (BeckmanCoulter, Marseille, France) according to the manufacturers’ protocols. These protocols are based on International Society of Hematotherapy and Graft Engineering (ISHAGE) Guidelines8 (link), and are frequently used for quantification of CD34+ cells mobilized into the peripheral blood. To increase the reproducibility of CD34+ cell counts, the protocol of the Stem-Kit was modified as follows: the blood sample volume, antibodies and lysing solution were doubled. After adding 30 μL of internal control (Stem count: BeckmanCoulter), samples were centrifuged for 5 min at 450 g, and 3,860 μL of supernatant was removed carefully with a pipet. Samples were analyzed by Coulter CYTOMICS™ FC500 and XL-system II software (BeckmanCoulter) for 6 min each.
Makino H., Miyamoto Y., Kikuchi-Taura A., Soma T., Taguchi A, & Kishimoto I. (2014). Decreased levels of circulating CD34+ cells are associated with coronary heart disease in Japanese patients with type 2 diabetes. Journal of Diabetes Investigation, 6(4), 473-478.
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2

Apoptosis Cell Surface Phosphatidylserine

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Surface exposure of PS by apoptotic cells was measured by flow cytometry with a Coulter Cytomics FC500 (Beckmann Coulter, USA) by adding Annexin V-FITC and propidium iodide (PI) to cells according to the manufacturer's instructions (Annexin-V Fluos, Roche Diagnostic, Italy).
Bortolozzi R., Viola G., Porcù E., Consolaro F., Marzano C., Pellei M., Gandin V, & Basso G. (2014). A novel copper(I) complex induces ER-stress-mediated apoptosis and sensitizes B-acute lymphoblastic leukemia cells to chemotherapeutic agents. Oncotarget, 5(15), 5978-5991.
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3

Apoptosis Detection by Flow Cytometry

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Cell death was determined by flow cytometry of cells double stained with annexin V/FITC and PI. The Coulter Cytomics FC500 (Beckman Coulter) was used to measure the surface exposure of PS on apoptotic cells according to the manufacturer’s instructions (Annexin-V Fluos, Roche Diagnostics).
Romagnoli R., Baraldi P.G., Salvador M.K., Prencipe F., Bertolasi V., Cancellieri M., Brancale A., Hamel E., Castagliuolo I., Consolaro F., Porcú E., Basso G, & Viola G. (2014). Synthesis, Antimitotic and Antivascular Activity of 1-(3′,4′,5′-Trimethoxybenzoyl)-3-arylamino-5-amino-1,2,4-triazoles. Journal of medicinal chemistry, 57(15), 6795-6808.
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4

Apoptosis Detection by Flow Cytometry

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Cell death was determined by flow cytometry of cells double stained with annexin V/FITC and PI. The Coulter Cytomics FC500 (Beckman Coulter) was used to measure the surface exposure of phosphatidylserine on apoptotic cells according to the manufacturer’s instructions (Annexin-V Fluos, Roche Diagnostics).
Romagnoli R., Baraldi P.G., Salvador M.K., Prencipe F., Lopez-Cara C., Ortega S.S., Brancale A., Hamel E., Castagliuolo I., Mitola S., Ronca R., Bortolozzi R., Porcù E., Basso G, & Viola G. (2015). Design, Synthesis, in Vitro, and in Vivo Anticancer and Antiangiogenic Activity of Novel 3-Arylaminobenzofuran Derivatives Targeting the Colchicine Site on Tubulin. Journal of medicinal chemistry, 58(7), 3209-3222.
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5

Apoptosis Detection via Flow Cytometry

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Surface exposure of phosphatidylserine on apoptotic cells was measured by flow cytometry with a Coulter Cytomics FC500 (Beckman Coulter) by adding Annexin V conjugated to fluorescein isothiocyanate (FITC) to cells according to the manufacturer's instructions (Annexin V Fluos, Roche Diagnostic). Simultaneously, the cells were stained with PI. Excitation was set at 488 nm, and the emission filters were at 525 and 585 nm, respectively, for FITC and PI.
CONSOLARO F., BASSO G., GHAEM-MAGAMI S., LAM E.W, & VIOLA G. (2015). FOXM1 is overexpressed in B-acute lymphoblastic leukemia (B-ALL) and its inhibition sensitizes B-ALL cells to chemotherapeutic drugs. International Journal of Oncology, 47(4), 1230-1240.
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6

Mitochondrial Potential Analysis by Flow Cytometry

Cited 1 time
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The analysis of the mitochondrial potential was carried out by flow cytometric analysis. Briefly, the cells treated with the test compound were labeled with the JC-1 dye as previously described [53 (link)]. The labeled cells were analyzed using the Coulter Cytomics FC500 (Beckman Coulter) in the FL1 and FL2 channel, respectively.
Romagnoli R., Oliva P., Prencipe F., Manfredini S., Budassi F., Brancale A., Ferla S., Hamel E., Corallo D., Aveic S., Manfreda L., Mariotto E., Bortolozzi R, & Viola G. (2022). Design, Synthesis and Biological Investigation of 2-Anilino Triazolopyrimidines as Tubulin Polymerization Inhibitors with Anticancer Activities. Pharmaceuticals, 15(8), 1031.
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7

Annexin-V-FITC Apoptosis Assay

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Surface exposure of PS on apoptotic cells was measured by flow cytometry with a Coulter Cytomics FC500 (Beckmann Coulter, USA) instrument by adding annexin-V-FITC and PI to cells according to the manufacturer′s instructions (annexin-V Fluos, Roche Diagnostic, Monza, Italy).
Carta D., Bortolozzi R., Hamel E., Basso G., Moro S., Viola G, & Ferlin M.G. (2015). Novel 3-Substituted 7-Phenylpyrrolo[3,2-f]quinolin-9(6H)-ones as Single Entities with Multitarget Antiproliferative Activity. Journal of medicinal chemistry, 58(20), 7991-8010.
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8

Annexin-V Apoptosis Quantification

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The quantification of the apoptosis induced by the test compounds was carried out by flow cytometric analysis using the Annexin-V Fluos kit (Roche Diagnostics, Rotkreuz, Switzerland) following the manufacturer’s instructions. The HeLa cells treated with the test compounds for different incubation times and at the indicated concentrations were then labeled with annexin V/FITC and PI and analyzed with Coulter Cytomics FC500 (Beckman Coulter) in the FL1 and FL3 channel, respectively.
Romagnoli R., Oliva P., Prencipe F., Manfredini S., Budassi F., Brancale A., Ferla S., Hamel E., Corallo D., Aveic S., Manfreda L., Mariotto E., Bortolozzi R, & Viola G. (2022). Design, Synthesis and Biological Investigation of 2-Anilino Triazolopyrimidines as Tubulin Polymerization Inhibitors with Anticancer Activities. Pharmaceuticals, 15(8), 1031.
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9

Apoptosis Detection by Flow Cytometry

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Cell death was determined by flow cytometry of cells double stained with annexin V/FITC
and PI. The Coulter Cytomics FC500 (Beckman Coulter) was used to measure the surface
exposure of phosphatidylserine on apoptotic cells according to the manufacturer’s
instructions (Annexin-V Fluos, Roche Diagnostics).
Spanò V., Rocca R., Barreca M., Giallombardo D., Montalbano A., Carbone A., Raimondi M.V., Gaudio E., Bortolozzi R., Bai R., Tassone P., Alcaro S., Hamel E., Viola G., Bertoni F, & Barraja P. (2020). Pyrrolo[2′,3′:3,4]cyclohepta[1,2-d][1,2]oxazoles, a New Class of Antimitotic Agents Active against Multiple Malignant Cell Types. Journal of Medicinal Chemistry, 63(20), 12023-12042.
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10

Apoptosis Evaluation by Flow Cytometry

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Cell death was determined by flow cytometry of cells double stained with annexin V/FITC and PI.
The Coulter Cytomics FC500 (Beckman Coulter) was used to measure the surface exposure of phosphatidyl serine on apoptotic cells according to the manufacturer's instructions (Annexin-V Fluos, Roche Diagnostics).
Spanò V., Barreca M., Rocca R., Bortolozzi R., Bai R., Carbone A., Raimondi M.V., Piccionello A.P., Montalbano A., Alcaro S., Hamel E., Viola G, & Barraja P. (2021). Insight on [1,3]thiazolo[4,5-e]isoindoles as tubulin polymerization inhibitors. European journal of medicinal chemistry, 212.
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