Juvenile birds received bilateral injection of 200–450 nL 6-OHDA solution into HVC at either ~30 d (mean ± SD: 30.1 ± 4.2 d, range: 25–34 d, n = 7) or ~45 d (mean ± SD: 44.5 ± 3.0 d, range: 39–47 d, n = 6). The solution was PBS-based and included 5–20 mM 6-OHDA hydrochloride (Santa Cruz, sc-203482), 10 mM L-ascorbic acid (MilliporeSigma, A92902), and 1 µM desipramine hydrochloride (Tocris, 3067), which was included as an inhibitor for noradrenaline and serotonin transporters to protect noradrenergic and serotonergic neurons at the injection site. Control birds received injection of PBS with 10 mM ascorbic acid and 1 µM desipramine at ~30 d (mean ± SD: 29.3 ± 3.6 d, range: 22–32 d, n = 7). Drugs were dissolved into PBS immediately before injection in place of equimolar NaCl (Working solution: ~300 mOsm, pH 7.3). After injection, birds were returned to their original home cage until ~45 d when they were isolated in a soundproof box until 90 d.
6 ohda hydrochloride
Manufactured by Santa Cruz Biotechnology
6-OHDA hydrochloride is a chemical compound commonly used in research applications. It is a neurotoxin that selectively damages dopaminergic and noradrenergic neurons. The compound is widely utilized in experimental models to induce Parkinson's-like symptoms.
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Lab products found in correlation
2 protocols using 6 ohda hydrochloride
1
Avian HVC Lesions via 6-OHDA Injection
2
Avian HVC Lesions via 6-OHDA Injection
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Juvenile birds received bilateral injection of 200–450 nL 6-OHDA solution into HVC at either ~30 d (mean ± SD: 30.1 ± 4.2 d, range: 25–34 d, n = 7) or ~45 d (mean ± SD: 44.5 ± 3.0 d, range: 39–47 d, n = 6). The solution was PBS-based and included 5–20 mM 6-OHDA hydrochloride (Santa Cruz, sc-203482), 10 mM L-ascorbic acid (MilliporeSigma, A92902), and 1 µM desipramine hydrochloride (Tocris, 3067), which was included as an inhibitor for noradrenaline and serotonin transporters to protect noradrenergic and serotonergic neurons at the injection site. Control birds received injection of PBS with 10 mM ascorbic acid and 1 µM desipramine at ~30 d (mean ± SD: 29.3 ± 3.6 d, range: 22–32 d, n = 7). Drugs were dissolved into PBS immediately before injection in place of equimolar NaCl (Working solution: ~300 mOsm, pH 7.3). After injection, birds were returned to their original home cage until ~45 d when they were isolated in a soundproof box until 90 d.
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