Log In Sign up
The largest database of trusted experimental protocols

Pmi micro stabilized rodent liquid diet ld 101

Manufactured by TestDiet
Visit Supplier
Sourced in United States

The PMI Micro-Stabilized Rodent Liquid Diet LD 101 is a laboratory product designed to provide a complete and balanced liquid nutrition for rodents. It is a sterile, ready-to-use formula that delivers essential nutrients, vitamins, and minerals in a convenient liquid form.

Automatically generated - may contain errors

Lab products found in correlation

C57bl 6j, by Jackson ImmunoResearch (1 mentions) Tamoxifen, by Merck Group (1 mentions) C57bl 6 mice, by Jackson ImmunoResearch (1 mentions)

7 protocols using pmi micro stabilized rodent liquid diet ld 101

1

Small Bowel Resection in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
All operated mice underwent a 50% proximal SBR or sham operation as we have previously described.[10 (link)] Briefly, through a midline laparotomy the bowel is exteriorized and then transected 12cm from the terminal ileum and 2–3cm from the pylorus. The intervening mesentery was ligated with silk suture and a hand sewn, end-to-end anastomosis was performed with interrupted 9-0 nylon sutures. For sham operations, the proximal bowel was transected 12cm from the terminal ileum and reaanastomosis performed (no resection). Animals were fasted for the first 24 hours post operatively then returned to standard liquid diet (LD; PMI Micro-Stabilized Rodent Liquid Diet LD 101; TestDiet, St. Louis MO). Animals were sacrificed at 5 or 10 weeks after operation. The small intestine, pancreas, and serum were collected.
Barron L., Courtney C., Bao J., Onufer E., Panni R.Z., Aladegbami B, & Warner B.W. (2018). Intestinal Resection-Associated Metabolic Syndrome. Journal of pediatric surgery, 53(6), 1142-1147.
+ Open protocol
+ Expand
2

Dietary Fat Modulation in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
C57BL/6J 11–12-week-old male mice from Jackson laboratories (Bar Harbor, ME) were housed in a temperature controlled, pathogen-free animal holding area on a 12-hour light-dark cycle. Mice were provided with water and either SC (13% kcal fat; PicoLab Rodent Diet 20, 53WU; LabDiet, St. Louis, MO) or liquid HFD (35% kcal fat; PMI Micro-Stabilized Rodent Liquid Diet LD 101; TestDiet,St. Louis, MO) ad libitum. In the HFD, the fat content is derived mainly from olive oil, corn oil, and safflower oil, which are highest in oleic acid, a monosaturated omega-9 long-chain fatty acid. In the SC diet, fat content is derived from soybean oil (composed mostly of polyunsaturated omega-6 linoleic acid) and, to a lesser extent, fish oil. Protocols and experiments were approved by the Washington University in St. Louis Animal Studies Committee (Protocol 20170252) in accordance with the National Institute of Health laboratory animal care and use guidelines.
Onufer E.J., Han Y.H., Czepielewski R.S., Courtney C.M., Sutton S., Randolph G.J, & Warner B.W. (2020). Effects of High Fat Diet on Liver Injury After Small Bowel Resection. Journal of pediatric surgery, 55(6), 1099-1106.
+ Open protocol
+ Expand
3

Intestinal Resection and Anastomosis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The 50% proximal SBR procedure has been previously described15 (link). The intestinal resections were performed by transecting the bowel 1 to 2 cm distal from the ligament of Treitz and at 12 cm proximal to the ileocecal junction followed by removal of the intervening segment. Intestinal continuity was re-established by an end-to-end primary anastomosis using interrupted 9-0 monofilament sutures. After the operation, mice were provided free access to water for the first 24 h after surgery. Mice were then fed with a standard liquid diet (PMI Micro-Stabilized Rodent Liquid Diet LD 101; Test Diet, Richmond IN) until sacrifice.
Aladegbami B., Barron L., Bao J., Colasanti J., Erwin C.R., Warner B.W, & Guo J. (2017). Epithelial cell specific Raptor is required for initiation of type 2 mucosal immunity in small intestine. Scientific Reports, 7, 5580.
+ Open protocol
+ Expand
4

Small Intestine Resection and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The 50% proximal SBR procedure has been described previously.19 (link) The intestinal resections were performed by transecting the bowel 1- to 2-cm distal from the ligament of Treitz and at 12-cm proximal to the ileocecal junction, followed by removal of the intervening segment. Intestinal continuity was re-established by an end-to-end primary anastomosis using interrupted 9-0 monofilament sutures. Mice were provided free access to water for the first 24 hours after surgery. Mice then were fed with a standard liquid diet (PMI Micro-Stabilized Rodent Liquid Diet LD 101; TestDiet, Richmond, IN) until death.
At the time of death, a midline laparotomy was performed and the entire small intestine was flushed with ice-cold phosphate-buffered saline containing protease inhibitors (0.2 nmol/L phenylmethylsulfonyl fluoride, 5 μg/mL aprotinin, 1 μmol/L benzamidine, 1 mmol/L sodium orthovanadate, and 2 μmol/L cantharidin). A 2-cm segment of bowel distal to the anastomosis was fixed in 10% neutral-buffered formalin for histology. The remainder of the distal segment was used to isolate crypt and villus.20 (link) Protein extracted from isolated crypt or villus was used for Western blot assay, and RNA was used for real-time polymerase chain reaction (PCR) assays.
Barron L., Sun R.C., Aladegbami B., Erwin C.R., Warner B.W, & Guo J. (2016). Intestinal Epithelial-Specific mTORC1 Activation Enhances Intestinal Adaptation After Small Bowel Resection. Cellular and Molecular Gastroenterology and Hepatology, 3(2), 231-244.
+ Open protocol
+ Expand
5

Genetic Labeling of Prox1-Expressing Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
As described previously, C57BL/6 J 9–12 week-old male mice were obtained from Jackson Laboratories (Bar Harbor, ME)15 (link). Prox1-Cre-ERT2 (Jax # 022075; originally generated by Srinivasan and Oliver31 (link)) crossed with Rosa26-tdTomatofl/fl reporter (Jax # 007905) 15–20 week male and female mice were orally treated with 20 mg/ml tamoxifen (Sigma, St. Louis, MO) dissolved in corn oil (50 µg/per gram body weight) every other day over two weeks, inducing Cre recombinase activity and downstream tdTomato expression. Mice were housed in a temperature controlled, specific pathogen-free unit on a 12-h light–dark cycle. All mice were fed a liquid diet (PMI Micro-Stabilized Rodent Liquid Diet LD 101; TestDiet, St. Louis, MO) and water ad libitum. This study was approved by the Washington University in St. Louis Animal Studies Committee (Protocol 20170252 and 20170154) in accordance with the National Institute of Health laboratory animal care and use guidelines. This study is reported in accordance to the ARRIVE guidelines32 (link).
Onufer E.J., Czepielewski R.S., Han Y.H., Courtney C.M., Sutton S., Sescleifer A., Randolph G.J, & Warner B.W. (2022). Lipid absorption and overall intestinal lymphatic transport are impaired following partial small bowel resection in mice. Scientific Reports, 12, 11527.
+ Open protocol
+ Expand
6

Small Bowel Resection in Mice

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
10-week-old male mice were fed solid pellet chow until 24 hours before procedures, at which time they were placed on a liquid diet (LD; PMI Micro-Stabilized Rodent Liquid Diet LD 101; TestDiet, St. Louis, MO, USA). On the day of surgery, preoperative weight was recorded, and a 1 cc normal saline bolus was administered intraperitoneally (IP). Operations were performed under isoflurane anesthesia with the mice positioned supine on a heating pad for the duration of the procedure. Sham operation (division of the bowel and reanastomosis without resection), 50% proximal SBR [15 (link)], or 50% distal SBR [21 (link)] were carried out as previously described, and additional 1 cc normal saline boluses were administered IP immediately and 24 hours postoperatively [4 (link)]. Liquid diet administration was restarted on postoperative day 1, and all animals were housed in an incubator for 1 week after operation. Liquid diet was continued until harvest. Weights were recorded weekly, and mice were harvested at both 2 and 10 week timepoints. Acceptable survival was ≥70%. Structural adaptation was confirmed by measuring villous height and crypt depth, which was previously published for these mice in our prior manuscripts. [18 , 21 (link)]
Gogol E.P., Johnston E., Aggeler R, & Capaldi R.A. (1990). Ligand-dependent structural variations in Escherichia coli F1 ATPase revealed by cryoelectron microscopy.. Proceedings of the National Academy of Sciences of the United States of America, 87(24), 9585-9589.
+ Open protocol
+ Expand
7

NAFLD Induction in C57BL6 Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
C57BL6 mice were obtained from Jackson Laboratories (Bar Harbor, ME) at 7 weeks of age. Mice were housed on arrival in a facility with a 12-h light/dark cycle. Male mice were used exclusively in order to limit hormonal confounders on the timing and severity NAFLD. (13 (link)) Male TLR4 KO mice were generously supplied by Dr. William Stenson (Division of Gastroenterology, Washington University School of Medicine in St. Louis). All mice were placed on liquid diet (LD; PMI Micro-Stabilized Rodent Liquid Diet LD 101; TestDiet, St. Louis MO) for 24 hours prior to operation until sacrifice. Food and water were provided ad libitum. This study was approved by the Washington University Animal Studies Committee (Protocol #20130308) in accordance with the National Institute of Health laboratory animal care and use guidelines.
Barron L.K., Bao J.W., Aladegbami B.G., Colasanti J.J., Guo J., Erwin C.R, & Warner B.W. (2017). Toll-Like Receptor 4 is Critical for the Development of Resection-Associated Steatosis. Journal of pediatric surgery, 52(6), 1014-1019.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!