B6.129 cg leprtm2 cre rck j

BKS.Cg-Lepr^db/J (JAX#000642, Homozygous, db/db), BKS.Cg-Dock7^m+/+/J (JAX#000642, Homozygous, WT), B6.Cg-Lep^ob/J (JAX#000632, Homozygous, ob/ob) B6.129(Cg)-Lepr^tm2(cre)Rck/J (JAX#008320, Homozygous, LepR^cre), B6.129P2-Lepr^tm1Rck/J (JAX#008327, Homozygous, lepR^fl/fl) and B6.Cg-Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/J (JAX#007914, Homozygous, Rosa26-CAG-loxp-stop-loxp-tdTomato) mice were obtained from the Jackson Laboratory. CCL19^Cre [Tg(Ccl19-cre)489Biat] mice were a gift from Shannon Turley at Genentech, South San Francisco, California, USA. All animal experiments and methods were performed in accordance with the relevant guidelines and regulations approved by the Institutional Animal Care and Use Committee of Brigham and Women’s Hospital, Boston, MA.

All experimental protocols were approved by the Johns Hopkins University Animal Use and Care Committee (Protocol # MO19M191) and by the George Washington University Animal Use and Care Committee (Protocols # A2023–009 and # A2023–014), and all animal experiments were conducted in accordance with ACUC guidelines. Lepr^b-Cre-GFP mice were generated by crossing LepR^b-Cre (B6.129(Cg)Lepr^tm2(cre)Rck/J; The Jackson Laboratory #008320) with GFP-floxed mice (B6; 129-Gt(ROSA)26Sor^tm2Sho/J; The Jackson Laboratory #004077). Water and food were available ad libitum. Mice were fed with a high fat diet (TD 03584, Teklad WI, 5.4 kcal/g, 35.2% fat, 58.4% kcal from fat) until males reached 40g and females reached 35g and used in our experiments at ~30 weeks of age. Aged-matched DIO mice on the C57BL/6J background were also used (The Jackson Laboratory #380050). Mice were housed at standard environmental conditions (24°C–26°C in the 12-h light/dark cycle, 9 a.m.–9 p.m. lights on), until the experiments started. For the in-vitro patch clamp experiments, all animal procedures were approved by the George Washington University Institutional Animal Care and Use Committee.

Mice were housed and bred under specific pathogen–free conditions at the UCSF Laboratory Animal Research Center. Male and female mice between 8 and 12 weeks of age were used for the experiments. WT C57BL/6J, C57BL/6-Tg(Pf4-icre)Q3Rsko/J, B6.Cg-Gt(ROSA)26Sor^{tm14(CAG-tdTdTomato)Hze}/J, B6.129(Cg)-Gt(ROSA)26Sor^{tm4(ACTB-tdTdTomato,-EGFP)Luo}/J, C57BL/6-Tg(UBC-GFP)30Scha/J, B6.129(Cg)-Lepr^tm2(cre)Rck/J and B6.129S2-CD40lg^tm1lmx/J mice were purchased from The Jackson Laboratory. c-mpl^−/− mice (on a C57BL/6 background) were obtained from Genentech through a material transfer agreement.