Protein was extracted from the hippocampus tissues according to standard Invent protocols (Invent, China) as in our previous study (Li et al., 2020 (link)). The total, nuclear, and total member proteins from the hippocampus tissues were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE), blotted and probed with the following antibodies: anti-IFITM3 (Abcam, ab15592, 1:1,000), anti-TLR4 (ProteinTech, 10,745-1-AP, 1:1,000), anti-NF-κB (ProteinTech, 66,350–1-Ig, 1:1,000), anti-β-actin (ProteinTech, 6,008–1-Ig, 1:2,000), anti-GAPDH (Abcam, ab8245, 1:2,000), anti-Na/K-ATPase (GeneTex, GTX30203), anti-cleaved caspase-8 (Abcam, ab25901, 1:1,000), and anti-cleaved caspase-3 (Abcam, ab13847, 1:1,000). Bradford assays (Bio-Rad Laboratories, Hercules, CA, United States) were used to quantify the protein concentrations. The blots were visualized with a chemiluminescence system (Amersham Bioscience, Buckinghamshire, United Kingdom), and the signals were quantified by densitometry. ImageJ was used to measured densities of blots, and the relative density was the ratio of target protein to GAPDH.
Gtx30203
Manufactured by Abcam
The GTX30203 is a laboratory equipment product that serves a core function. This description is factual and unbiased, without any interpretation or extrapolation on its intended use.
Automatically generated - may contain errors
2 protocols using gtx30203
1
Hippocampus Protein Extraction and Analysis
2
Hippocampus Protein Extraction and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Protein was extracted from the hippocampus tissues according to standard Invent protocols (Invent, China) as in our previous study (Li et al., 2020 (link)). The total, nuclear, and total member proteins from the hippocampus tissues were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE), blotted and probed with the following antibodies: anti-IFITM3 (Abcam, ab15592, 1:1,000), anti-TLR4 (ProteinTech, 10,745-1-AP, 1:1,000), anti-NF-κB (ProteinTech, 66,350–1-Ig, 1:1,000), anti-β-actin (ProteinTech, 6,008–1-Ig, 1:2,000), anti-GAPDH (Abcam, ab8245, 1:2,000), anti-Na/K-ATPase (GeneTex, GTX30203), anti-cleaved caspase-8 (Abcam, ab25901, 1:1,000), and anti-cleaved caspase-3 (Abcam, ab13847, 1:1,000). Bradford assays (Bio-Rad Laboratories, Hercules, CA, United States) were used to quantify the protein concentrations. The blots were visualized with a chemiluminescence system (Amersham Bioscience, Buckinghamshire, United Kingdom), and the signals were quantified by densitometry. ImageJ was used to measured densities of blots, and the relative density was the ratio of target protein to GAPDH.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!