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12 protocols using bacl2

1

Characterization of Potassium Sulfate Fertilizer

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Agricultural potassium sulfate (LN K2SO4) was obtained from SDIC Xinjiang Lop Nur Potash Co. Ltd., China. Chemical grade polyethylene glycol 1000 (PEG 1000), sodium tripolyphosphate (STPP), and analytical grade reagents for gravimetric methods and Volhard method (sodium tetraphenylborate, ethylenediaminetetraacetic acid disodium salt, ammonium iron sulfate, BaCl2 and AgNO3) were purchased from Sinopharm Chemical Reagent Co. Ltd. Industrial grade urea phosphate (UP) was purchased from Tianjin Kemiou Chemical Reagent Co. Ltd. Tap water was used in all experiments and its physicochemical properties are shown in Table 1. The concentration of metal ions (K+, Na+, Mg2+, Ca2+) was determined by an atomic absorption spectrophotometer (AAS, AA-6880 SHIMADZU, Japan). The concentration of SO42− and Cl anions was determined by the barium chloride gravimetric method and the Volhard method, respectively.
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2

Mung Bean Seed Heavy Metal Uptake

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MA, urea, K2Cr2O7, ascorbic acid, NaOH and HCl were purchased from Aladdin Chemical Co., Ltd (Shanghai, China). Other chemicals, including NaCl, KCl, AgNO3, CuCl2·2H2O, CaCl2·2H2O, FeCl2, MnSO4·H2O, BaCl2, NiCl2·6H2O, MgCl2, Zn(NO3)2, FeCl3·6H2O, Al(NO3)3·9H2O and CrCl3·6H2O, were obtained from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Mung bean seeds were purchased from retail outlets. All the chemical agents were of analytical grade and used as received without further purification. Deionized water was used for preparing the solutions.
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3

Cytotoxicity Screening of Metal Salts

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SGI (10,000×) was purchased from Beijing Solarbio Science and Technology Co., Ltd. (Beijing, China) and diluted using DMSO to a final concentration of 100× or 10× as the stock solution. It is noted that SGI 1× was equivalent to a concentration of 1.96 μM. The analytical-grade AgNO3, KCl, LiCl, CaCl2, MgCl2, MnCl2, CoCl2, CuSO4, BaCl2, and NiSO4 were purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China) and dissolved using DI water to a final concentration of 50 μM as the stock solutions.
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4

Thorium Compounds Handling and Characterization

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Caution! Th-232 and its daughter nucleus Ra-228 are both radioactive. All of the thorium compounds used and investigated were operated in an authorized laboratory designed for actinide element studies. Standard precautions for radioactive materials should be followed.All reagents were purchased from chemical reagent suppliers and used without further purification. N,N′-Dimethylformamide (DMF, 99%), formic acid (98%+), n-hexane (97%, SafeDry), 1,4-dioxane (99%+), NaIO3 (99%), AlCl3 (99.99%), Na2CO3 (99.95%), and NaHCO3 (99%, Adamas) were provided by Adamas. Na2SO4 (≥99.0%), NaF (99%), NaCl (≥99.5%), MeOH (≥99.5%), and acetonitrile (CH3CN, ≥99.0%) were from Greagent. Concentrated hydrochloric acid (36.0–38.0%), ethanol (≥99.7%), acetone (99.5%), ethyl ether (99%), NaCl (99%), KCl (99%), BaCl2 (99%), SrCl2 (99%), CaCl2 (99%), ZnCl2 (99%), MgCl2 (99%), and Na3BO3 (99%) were provided by Sinopharm Chemical Reagent Co., Ltd. Other reagents: Th(NO3)4·6H2O (99%, Changchun Institute of Applied Chemistry, CAS) and tris((4-carboxyl)phenylduryl)amine (98%, Jilin Chinese Academy of Sciences – Yanshen Technology Co., Ltd.).
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5

Enzymatic Assay Protocol for Trypsin

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Trypsin
from bovine pancreas was obtained
from Aladdin Co., Ltd (Shanghai, China). CuSO4·5H2O was purchased from Shanghai Bodi Chemical Co., Ltd (Shanghai,
China). HgCl2, KCl, CaCl2, MgCl2,
MnCl2, CoCl2, BaCl2, CuCl2, NiCl2, FeCl2, FeCl3, and AlCl3 were purchased from Sinopharm Chemical Reagent Co., Ltd.
(Shanghai, China). l-Tryptophan (l-Trp), l-proline (l-Pro), l-tyrosine (l-Tyr), l-histidine (l-His), l-threonine (l-Thr), and l-phenylalanine (l-Phe) were purchased
from Shanghai Sangon Biotechnology Co., Ltd. (Shanghai, China).
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6

Synthesis and Characterization of Graphene-Based Nanocomposites

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UPE was obtained
from Shanghai Lianle Chemical Technology Co., Ltd (Shanghai, China),
with a maximum particle size of 250 μm. The NG powder was purchased
from Qingdao Xinghe Graphite Co., Ltd (Shandong, China), with an average
particle size of about 30 μm and a purity of 99.85%. CNF was
obtained from Showa Denko, Japan. DMF, KMnO4, K2S2O8, P2O5, and H2SO4 (98%) were all purchased from Shanghai Lingfeng
Chemical Reagent Co., Ltd. BaCl2, HCl, C2H5OH, H2O2 (30%), and N2H4·H2O) (60%) were purchased from Sinopharm
Chemical Reagent Co., Ltd. Deionized water was made in the laboratory.
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7

Preparation of Metal-Chitosan Composites

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l-Valine (Val), phosphoric acid, NaOH,
FeCl2, FeCl3, ethanol, K2SO4, K3PO4, K2CO3, Hg(NO3)2, AgNO3, ZnCl2, CuSO4, CdCl2, BaCl2, Pb(NO3)2, MnCl2, CaCl2, MgCl2, Cr2(SO4)3, and K2Cr2O7 were bought from Sinopharm Chemical Reagent Co. Ltd.
(Beijing, China).
Poly(ethylene oxide) (average Mw 900 kDa)
and Chitosan (average Mw 200 kDa) were
obtained from Sigma-Aldrich. Dialysis tubing with a flat diameter
of 28 mm and a molecular weight cutoff (MWCO) of 1 kDa were provided
by Biotopped (Viskase). Deionized water with a resistivity of 18.2
MΩ was provided by a GWA-UN water purification system. All materials
and chemicals were of analytical grade and used as received without
further purification.
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8

Ornithine-Based Bioactive Compound Analysis

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l-Ornithine hydrochloride was obtained from Sigma-Aldrich Co. Ltd (Shanghai, China). FeCl3, NaCl, KCl, CaCl2, CuCl2, MgCl2, FeCl2, Zn(NO3)2, BaCl2, Pb(NO3)2, AlCl3, NiSO4, CoCl2, Cr2(SO3)3, CdSO4, Hg(NO3)2, MnCl2, MTT, Dulbecco's modified Eagle medium (DMEM) and quinine sulfate were purchased from Sinopharm Chemical Reagent Co. Ltd. RPMI-1640 medium was obtained from Gibco Company Ltd (USA). Fetal bovine serum was purchased from Hyclone Company Ltd (USA). A549 cells were purchased from the American Type Culture Collection (ATCC). Sephadex G-25 was purchased from GE Healthcare. All other chemical reagents were of analytical grade and used without any further purification. Ultrapure water (18.2 MΩ cm) was prepared using a Milli-Q system.
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9

Functionalized Polymer-Anthracene Conjugates

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Fresh holly leaves were purchased from a local market. Polyethyleneimine (PEI1.8k), N-hydrosuccinidyl ester-functionalized homobifunctional poly(ethylene glycol) (NHS-PEG2000-NHS) and 9,10-bis(phenylethynyl)-anthracene (BPEA) were obtained from Shanghai Aladdin Reagent Co., Ltd (China). A dialysis membrane (cutoff molecular weight ~3500) was purchased from Spectrum Laboratories, Inc., CA (USA). HgCl2, KCl, Pb(NO3)2, CaCl2, NaCl, AgNO3, BaCl2, CoCl2, MgCl2, CuCl2, FeCl2 and FeCl3 were purchased from Sinopharm Chemical Reagent Co., Ltd. (China). And 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide (MTT) was obtained from Sigma-Aldrich, St. Louis, MO (USA).
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10

Synthesis of 3,5-diaminobenzoic Acid Derivatives

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3,5-diaminobenzoic acid was purchased from Aladdin Industrial Corporation. Ethanol (≥99.7%), methanol (≥99.8%), dichloromethane (≥99.5%), and silica gel (Acuity FCP. 300–400 mesh) were obtained from Sinopharm Group Chemical Reagent Co., Ltd., Shanghai, China. All reagents were analytical reagent grade. BaCl2, CaCl2, CrCl2, CuCl2, FeCl2, FeCl3, KCl, MgCl2, MnCl2, NaCl, PbCl2, NaOH, HCl, NaH2PO4, Na2HPO4, citric acid, sodium citrate dihydrate (C6H5Na3O7·2H2O), and Tris were all purchased from Sinopharm Group Chemical Reagent Co., Ltd. Deionized (DI) water was used throughout the experiments. All aqueous solutions were prepared with ultrapure water (≥18.2 MΩ·cm) from a Labonova Smart system (Think-lab, Germany).
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