Small RNA sequencing libraries were prepared from RNA extracted from WT and Tet2−/− (Fig. 5 ) or from E14 mESCs and Tet2−/− (Extended Data Fig. 5 ). Total RNA was extracted using TRIzol (Thermo Fisher Scientific) and 80% EtOH washes were performed during precipitation. For small RNA cloning, total RNA was size selected for 14–38 nts on 15% Novex TBE urea gels (Thermo Fisher Scientific). Small RNA libraries were prepared using the Illumina TruSeq small RNA kit (Fig. 5 ) or the NEB small RNA-seq library kit (Extended Data Fig. 5 ) and sequenced on a NextSeq 500 platform.
Small rna seq library kit
Manufactured by New England Biolabs
The Small RNA-seq library kit is a reagent system designed for the preparation of small RNA libraries for next-generation sequencing. The kit includes enzymes, buffers, and adapters necessary for the reverse transcription, ligation, and amplification of small RNA molecules, enabling their sequencing analysis.
Automatically generated - may contain errors
2 protocols using small rna seq library kit
1
Small RNA Sequencing of Tet2-Deficient Cells
2
Small RNA Sequencing of Tet2-Deficient Cells
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