Af0306

The Co-IP was conducted as described previously (Chen et al., 2020 ). The cells were co-transfected with HA-tagged IFNAR1 and Flag-tagged CK1α. After 48 ​h of transfection, the cells were lysed in RIPA lysis buffer, incubated with protein G agarose beads, then immunoprecipitated with anti-HA antibody or anti-Flag antibody at 4 ​°C overnight. The immunoprecipitated proteins were analyzed by Western blot with indicated antibodies. The following antibodies were used: CK1α (1:500, 55192-1-AP, rabbit, Proteintech, Rosemont, IL,USA), IFNAR1 (1:500, ab45172, rabbit, Abcam, Cambridge, MA, USA), STAT1 (1:1000, 14994, rabbit, CST, Danvers, MA, USA), pSTAT1 (1: 1000, 7649, rabbit, CST), STAT2 (1:1000, 72604, rabbit, CST), p-STAT2 (1:1000, 88410, rabbit, CST), Flag-tag (1:1000, MA-1-91878, mouse, Thermo, San Jose, CA, USA), HA-tag (1:1000, 26183, mouse, Thermo), β-actin (1:3000, sc-47778, mouse, Santa Cruz, Dallas, TX, USA), and ubiquitin (1:500, AF0306, rabbit, Beyotime, Shanghai, China). The mouse monoclonal antibody against HBV core protein (HBc) was a kind gift from Prof. Xuefei Cai in our lab. The density of the immunoblot band was analyzed by Image J (National Institutes of Health, Bethesda, MD, USA).