Bact alert culture media

Microbial cultures were used to determine the type of organism present in different biological specimens derived from different site (respiratory tract, urinary tract, abdomen, and others) according to clinical suspicion and test requested to the laboratory by standardized microbiological cultural assays procedures.
Blood cultures were performed in patients with clinical symptoms of bloodstream infections prior the administration of antimicrobial therapy. For each patient, two bottles sets were used for each septic episode; approximately 10 mL of blood was inoculated in the aerobic and anaerobic bottle (BACT/ALERT Culture Media, Biomerieux, Marcy-l’Etoile, France); the bottles were entered in the BACT/ALERT 3D System for the incubation and measure of the color change in response to shift in pH as a result of rising of CO₂ levels produced by microorganisms. In positive samples, bacteria and yeasts were identified on the Vitek 2 system (Biomerieux, Marcy-l’Etoile, France), according to manufacturer directions.

Blood cultures were performed in patients with clinical symptoms of blood stream infections prior the administration of antimicrobial therapy. For each patient, at least two bottle sets were used for each septic episode; approximately 10 mL of blood was inoculated in the aerobic and anaerobic bottle (BACT/ALERT Culture Media, Biomerieux, Marcy-l’Etoile, France); the bottles were entered in the BACT/ALERT 3D System for the incubation and measure of the color change in response to shift in pH as a result of rising of CO₂ levels produced by microorganisms. In positive samples, bacteria and yeasts were identified on the Vitek 2 system (Biomerieux, Marcy-l’Etoile, France), according to manufacturer directions.