40,000 cells in serum-free media were added to the top chamber of an 8.0 μm PET cell culture insert (Falcon) with conditioned media (72 hr) from indicated cells in the bottom reservoir. After 12 (HeyA8) or 18 (TYK-nu) hr cells were fixed with 4% PFA in PBS, representative fluorescent images collected with a Zeiss Axiovert Observer.A1, stained with 0.1% crystal violet, washed extensively with PBS, and dried. Crystal violet was released with 10% acetic acid and the absorbance at 520 nm measured to quantify relative migration.
Axiovert observer a1
Manufactured by Zeiss
The Axiovert Observer.A1 is a high-performance inverted microscope designed for a variety of laboratory applications. It features a sturdy, ergonomic design and delivers reliable performance for routine imaging and analysis tasks.
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Lab products found in correlation
4 protocols using axiovert observer a1
1
Cell Migration Assay Protocol
2
Ovarian Cancer Cell Proliferation Assay
3
Cell Migration Assay Protocol
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40,000 cells in serum-free media were added to the top chamber of an 8.0 μm PET cell culture insert (Falcon) with conditioned media (72 hr) from indicated cells in the bottom reservoir. After 12 (HeyA8) or 18 (TYK-nu) hr cells were fixed with 4% PFA in PBS, representative fluorescent images collected with a Zeiss Axiovert Observer.A1, stained with 0.1% crystal violet, washed extensively with PBS, and dried. Crystal violet was released with 10% acetic acid and the absorbance at 520 nm measured to quantify relative migration.
4
Ovarian Cancer Cell Proliferation Assay
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Ovarian cancer cells were seeded at 1,000 cells per well in a 96-well plate and allowed to adhere for 24 hr before addition of conditioned media (72 hr) from the indicated cells. After 24 hr and 48 hr, nuclei were visualized by addition of Hoechst 33258 (1:5000) and wells fluorescently imaged with a Zeiss Axiovert Observer.A1 to extract cell number and doubling time. Images were analyzed with ImageJ 1.50j.
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