Glucose liquicolor test

Manufactured by EKF Diagnostics

Sourced in United States

The Glucose LiquiColor Test is a laboratory diagnostic product manufactured by EKF Diagnostics. It is used to quantitatively measure the concentration of glucose in a sample.

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Lab products found in correlation

Mouse albumin elisa kit, by Fortis Life Sciences (2 mentions) Aeroset clinical chemistry system, by Abbott (2 mentions) Gro pap method, by Nanjing Jiancheng (2 mentions) Au5800, by Beckman Coulter (1 mentions) Rat mouse insulin elisa kit, by Merck Group (1 mentions) Triglycerides liquicolor test, by EKF Diagnostics (1 mentions) Creatinine assay kit, by BioAssay Systems (1 mentions) Urea assay kit, by BioAssay Systems (1 mentions)

Spelling variants of this product

Glucose LiquiColor®, (3 citations)

7 protocols using glucose liquicolor test

Evaluating Renal Function Biomarkers

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The collected blood samples were centrifuged at 5000 g at 4°C for 10 min. Then, the serum was collected, and the level of blood glucose was determined using a Glucose LiquiColor^® Test (Stanbio Laboratory, Boerne, TX, USA), while the serum creatinine level was determined using an automatic biochemical analyzer (AU5800, Beckman Coulter Inc., Brea, CA, USA).
Rats were kept separately in metabolic cages to have the urine samples collected. The 24-h urinary protein level was evaluated using an enzyme-linked immunosorbent assay (ELISA) kit (JianglaiBio, Shanghai, China) according to the kit’s instructions. In addition, the urine creatinine level was examined using an automatic biochemical instrument, and the urinary microalbumin was examined using a rat microalbuminuria (MAU/ALB) ELSA kit (CSB-E12991r, USCN kit Inc., Hubei, China). Then, the urinary albumin creatinine ratio (UACR) was calculated.

Hao Y., Miao J., Liu W., Cai K., Huang X, & Peng L. (2021). Mesenchymal Stem Cell-Derived Exosomes Carry MicroRNA-125a to Protect Against Diabetic Nephropathy by Targeting Histone Deacetylase 1 and Downregulating Endothelin-1. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 14, 1405-1418.

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Metabolic Monitoring in Fasted Mice

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After mice were fasted overnight, blood glucose level was measured by Glucose LiquiColor^® Test (Stanbio Laboratory, Boerne, TX, USA) every 4 weeks. Twenty‐four‐hour urine was collected by metabolic cage every 4 weeks. Serum creatinine, BUN, and urinary creatinine were determined on an AEROSET Clinical Chemistry System (Abbott Laboratories, Chicago, IL, USA). Urine albumin concentration was determined by the mouse albumin ELISA kit (Bethyl Laboratories, Montgomery, TX, USA).

Zhao Y., Yin Z., Li H., Fan J., Yang S., Chen C, & Wang D.W. (2017). MiR‐30c protects diabetic nephropathy by suppressing epithelial‐to‐mesenchymal transition in db/db mice. Aging Cell, 16(2), 387-400.

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Molecular Mechanisms of Glucose Homeostasis

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Blood glucose was tested using the Glucose LiquiColor Test (Stanbio Laboratory, Boerne, TX, USA). 24-hour urine was obtained using the metabolic cage every four weeks. Serum creatinine, BUN level, and urinary creatinine level were examined on an AEROSET clinical chemistry system (Abbott Laboratories, Chicago, IL, USA). Urine albumin concentration was detected using the mice albumin ELISA kit (Bethyl Laboratories, Montgomery, TX, USA).

Zhu Y., Xu J., Liang W., Li J., Feng L., Zheng P., Ji T, & Bai S. (2019). miR-98-5p Alleviated Epithelial-to-Mesenchymal Transition and Renal Fibrosis via Targeting Hmga2 in Diabetic Nephropathy. International Journal of Endocrinology, 2019, 4946181.

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Metabolic Biomarker Measurements

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Serum glucose (Glucose Liquicolor test, Stanbio Laboratory, Boerne, Texas, USA), insulin (Rat/mouse insulin ELISA kit, Millipore Sigma, Burlington, Ontario, Canada) and triglycerides (Triglycerides Liquicolor test, Stanbio Laboratory, Boerne, Texas, US) were measured using commercially available ELISA-based kits following the manufactures instructions. Homeostatic Model Assessment of Insulin Resistance (HOMA) was calculated based on the standard formula (HOMA-IR = (fasting serum insulin (μU/mL)*fasting serum glucose (mmol/L)/22.5).

Mayengbam S., Chleilat F, & Reimer R.A. (2020). Dietary Vitamin B6 Deficiency Impairs Gut Microbiota and Host and Microbial Metabolites in Rats. Biomedicines, 8(11), 469.

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Comprehensive Metabolic Monitoring in Mice

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Fasting blood glucose was measured by Glucose LiquiColor Test (Stanbio Laboratory, Boerne, TX) every 2 weeks. 24-hour urine volume of each animal was collected using metabolic cage system every 4 weeks. Serum creatinine was detected using the Creatinine Assay Kit (BioAssay System, CA) with an improved Jaffe method, while BUN was detected using the Urea Assay Kit (BioAssay System, CA) with an improved Jung method. Urine albumin was detected using the mouse albumin ELISA kit (Bethyl Laboratories, Montgomery, TX).

He M., Wang J., Yin Z., Zhao Y., Hou H., Fan J., Li H., Wen Z., Tang J., Wang Y., Wang D.W, & Chen C. (2019). MiR-320a induces diabetic nephropathy via inhibiting MafB. Aging (Albany NY), 11(10), 3055-3079.

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Comprehensive lipid and glucose analysis

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Biochemical parameters TC, TG, LDL, and HDL in plasma, TC and TG in heart or H9c2 cells were detected by GRO-PAP method (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). After mice were fasted overnight, blood glucose level was measured by Glucose LiquiColor Test (Stanbio Laboratory, Boerne, TX) every 4 weeks.

Dai B., Li H., Fan J., Zhao Y., Yin Z., Nie X., Wang D.W, & Chen C. (2018). MiR-21 protected against diabetic cardiomyopathy induced diastolic dysfunction by targeting gelsolin. Cardiovascular Diabetology, 17, 123.

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Lipid and Glucose Biomarkers Measurement

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Circulating Triglyceride (TG), total cholesterol (TC) levels were detected using GRO-PAP method (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). Mice were fasted overnight, then blood glucose levels were measured by Glucose LiquiColor Test (Stanbio Laboratory, Boerne, TX). Hepatic triglyceride levels were determined using liver homogenate by GRO-PAP method (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) after mice were sacrificed.

Zhan J., Lv H., Dai B., Yuan S., Fan J., Zhao Y., Yin Z., Wang D.W., Chen C, & Li H. (2020). The nuclear and cytoplasmic roles of miR-320 in non-alcoholic fatty liver disease. Aging, 12(21).

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