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Papillocheck

Manufactured by Greiner
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Sourced in Germany

PapilloCheck is a piece of laboratory equipment. It is designed for a specific purpose, but a detailed and unbiased description of its core function cannot be provided while maintaining conciseness and avoiding extrapolation.

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Lab products found in correlation

Nanodrop uv spectrophotometer, by Thermo Fisher Scientific (1 mentions) Qiaamp dna mini kit, by Qiagen (1 mentions) Nucleospin tissue kit, by Macherey-Nagel (1 mentions) Linear array hpv genotyping, by Roche (1 mentions)

Spelling variants of this product

PapilloCheck® Test Kit (2 citations) PapilloCheck® HPV-Screening (2 citations) PapilloCheck HPV genotyping test (2 citations)

5 protocols using papillocheck

1

HPV Genotyping Using PapilloCheck Microarray

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All positive HPV PCR-Multiplex samples were submitted to PapilloCheck® microarray (Greiner Bio-One, Germany) 5μL of DNA eluate was used in the PapilloCheck® microarray for each reaction. Specimens containing the target DNA are hybridized to specific oligonucleotide probes immobilized on a DNA chip and detected by the binding of a Cy5-dUTP labeled oligonucleotide probe to the tag sequence. The DNA chip was scanned by the CheckScanner apparatus at 532 and 635 nm wave lengths. This test detects HPV genotypes 6, 11, 16, 18, 31, 33, 35, 39, 40, 42, 43, 44, 45, 51, 52, 53.56, 58, 59, 66, 68, 70, 73, and 82. In addition, human ADAT1 gene (adenosine deaminase, tRNA specific 1) was used as an internal control to assess the quality of the DNA
Golfetto L., Alves E.V., Martins T.R., Sincero T.C., Castro J.B., Dannebrock C., Oliveira J.G., Levi J.E., Onofre A.S, & Bazzo M.L. (2018). PCR-RFLP assay as an option for primary HPV test. Brazilian Journal of Medical and Biological Research, 51(5), e7098.
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2

HPV Genotyping Using PapilloCheck

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DNA was quantified using a Nanodrop UV spectrophotometer (Thermo Fisher, Altrincham, Cheshire, UK) and used for HPV genotyping with the PapilloCheck® test (Greiner Bio-One, Stonehouse, Gloucestershire, UK) as per the manufacturer’s instructions. This identifies 24 different HPV genotypes: six low-risk HPV types (LR: 6, 11, 40, 42, 43, and 44/55) and 18 high-risk HPV types (HR: 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 70, 73, and 82).
He X., Maranga I.O., Oliver A.W., Gichangi P., Hampson L, & Hampson I.N. (2016). Analysis of the Prevalence of HTLV-1 Proviral DNA in Cervical Smears and Carcinomas from HIV Positive and Negative Kenyan Women. Viruses, 8(9), 245.
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3

HPV Genotyping Using PapilloCheck

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Testing was undertaken using the PapilloCheck® (Greiner Bio-One, Kremsmiinster, Austria) platform. After viral and human DNA were extracted from the swab specimen, a 350 base-pair fragment of the HPV E1 gene was amplified in the presence of HPV-specific primers by polymerase chain reaction and the amplified products were hybridized to specific DNA probes fixed on a DNA chip. Both LR (6,11,40,42,43,44) and HR (16,18,31,33,35,39,45,51,52,53,56,58,59,66,68,70,73,82) HPV types were reported.
Cranston R.D., Carballo-Diéguez A., Gundacker H., Richardson B.A., Giguere R., Dolezal C., Siegel A., KunjaraNaAyudhya R.P., Gomez K., Piper J.M., Lama J.R, & McGowan I. (2018). Prevalence and determinants of anal human papillomavirus infection in men who have sex with men and transgender women. International journal of STD & AIDS, 30(2), 154-162.
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4

HPV, EBV, HCMV, HAdV Detection in LSCC

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Combined PCR/microarrays (PapilloCheck®, Greiner BioOneGmbH, Frickenhausen, Germany) were used for detection and genotyping of HPV and real-time PCR for EBV, HCMV and HAdV DNA detection. EBER in situ hybridization (EBER-ISH) was used for EBV detection in slides from LSCC tumors. In addition, we used immunohistochemistry (IHC) for mapping of p16 (Fig. 1).
Schindele A., Holm A., Nylander K., Allard A, & Olofsson K. (2022). Mapping human papillomavirus, Epstein–Barr virus, cytomegalovirus, adenovirus, and p16 in laryngeal cancer. Discover. Oncology, 13, 18.
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5

HPV16-Associated Invasive Cervical Cancer

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Ten invasive cervical cancers containing HPV16 DNA as single infection, determined after Linear Array HPV Genotyping (Roche Molecular Diagnostics, Pleasanton, USA) or PapilloCheck (Greiner Bio-One GmbH, Frickenhausen, Germany) tests were selected for the present study. The ICC cases originated from women participating in a previous study (de Oliveira et al., 2013) , and the condyloma originated from a women attended at the Gynecological Department from Hospital das Clínicas -Universidade de São Paulo. The study was approved by the Brazilian National Research Ethical Committee (CONEP n°158.490).
Total DNA was extracted from fresh tissue using QIAamp DNA Mini Kit (Qiagen, Gaithersburg, USA) or NucleoSpin Tissue kit (Macherey-Nagel GmbH&Co, Germany), according to manufacturer's instructions.
de Oliveira C.M., Bravo I.G., Santiago e Souza N.C., Genta M.L., Fregnani J.H., Tacla M., Carvalho J.P., Longatto-Filho A, & Levi J.E. (2015). High-level of viral genomic diversity in cervical cancers: A Brazilian study on human papillomavirus type 16. Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 34.
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