Bombesin (GRPr agonist) was purchased from the Anaspec Company (Fremont, CA). A GRPr antagonist (G-Abz4-STAT), which binds to the receptor with high affinity was obtained from the laboratory of Dr. Tweedle (The Ohio State University). Five frozen tumor specimens from dog primary prostate cancers (OC, CB, FM, Probasco, and LuMa) were obtained from The Ohio State University (OSU) College of Veterinary Medicine Biospecimen Repository. The Ace-1 cell line has been developed in our lab from a primary prostatic carcinoma of an 8-year-old male castrated Labrador retriever [21 (link)]. The Ace-1 cells form mixed osteoblastic/osteolytic bone metastases in nude mice [21 (link)–24 (link)]. The cloned Ace-1-huGRPr cells were provided by coauthor, Dr. Michael Tweedle. A high expressing clone (clone 15) was selected based on cell binding assay using 125I-Tyr4-BBN and expression of huGRPr mRNA. Each cell expressed approximately 690,000 cell membrane receptors. The Ace-1 and Ace- 1-huGRPr cells were stably transfected with luciferase so that the cells could be imaged in vivo using bioluminescence [25 (link)]. The cell lines were cultured in DMEM/F12 containing 10% FBS and antibiotics and maintained at 37°C, 5% CO2, and 100% humidity.
Bombesin
Manufactured by AnaSpec
Sourced in United States
Bombesin is a 14-amino acid peptide that functions as a neuropeptide and mitogen. It stimulates the release of gastrointestinal hormones and has a role in regulating gastrointestinal function.
Automatically generated - may contain errors
2 protocols using bombesin
1
Prostate Cancer Xenograft Model
2
Synthetic Peptide Acquisition and Synthesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Synthetic human Aβ1-42 (catalog number AS-24224), Aβ1-40 (catalog number AS-24236), SST14 (catalog number AS-24277), [Arg8]-Vasopressin (catalog number AS-24289), Bombesin (catalog number AS-20665), Oxytocin (catalog number AS-24275), SST28 (catalog number AS-22902) and amylin1-37 (catalog number AS-60804) were purchased from Anaspec, Inc. (Fremont, CA, USA). The alternative synthetic human SST14 peptide (catalog number H-1490) sourced from a different vendor (see Figure 4B ), CST17 (catalog number H-5536) and CST29 (catalog number H-6458) were obtained from Bachem Americas, Inc. (Torrance, CA, USA). The biotinylated Aβ peptides, including biotin-Aβ1-42, Aβ1-42-biotin, Aβ17-42-biotin, biotin-SST14 and truncated or mutant SST peptides were synthesized by LifeTein LLC (Hillsborough, NJ, USA). The Edans-SST donor and Aβ1-42-TMR acceptor peptides were in-house synthesized as described previously (Bateman and Chakrabartty, 2011 (link)).
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