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8 protocols using cps532

1

Characterization of MDNN Focusing Effect

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The experimental setup for the MDNN characterization is presented in Supplementary Fig. S10. A laser diode emitting at 532 nm (Thorlabs CPS532) was utilized as the input light. A linear polarizer was used to create the desired polarizations. The light is then directed onto the metasurface and imaged on a CMOS camera DCC3260C through a 100× objective lens. Videos of the MDNN focusing effect with diffraction distance are obtained by the movement of a stepper motor. Since the metasurface is integrated onto the CMOS imaging sensor, the output images of the experiment in Fig. 5 are collected directly by the image sensor on the CMOS chip (Sony IMX686).
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2

Spatially Modulated Laser Illumination for Multimode Fiber Characterization

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The experimental setup is illustrated in Fig. 2. The light source was a collimated diode-pumped solid-state laser module (532 nm, 4.5 mW, CPS532, Thorlabs, New Jersey, USA). After beam expansion through two achromatic lenses (AC254-030-A-ML; AC254-075-A-ML, Thorlabs, New Jersey, USA), the laser light was spatially modulated using a DMD (DLP7000, Texas Instruments, Texas, USA) and then projected onto the proximal facet of a MMF (105 μm, 0.22 NA, 1 m, M43L01, Thorlabs, New Jersey, USA) via a tube lens (AC254-050-A-ML, Thorlabs, New Jersey, USA) and an objective (20×, 0.4 NA, RMS20X, Thorlabs, New Jersey, USA). The light illuminated area on the DMD included 32 × 32 independent input modes, with each 2 × 2 micromirrors grouped as one mode. An objective (20×, 0.4 NA, RMS20X, Thorlabs, New Jersey, USA) and a tube lens (AC254-0100-A-ML, Thorlabs, New Jersey, USA) were used to magnify the output light beam before it was captured by a complementary metal-oxide-semiconductor (CMOS) camera (C11440-22CU01, Hamamatsu Photonics, Shizuoka, Japan) with a frame rate of 200 frames per second (fps) for MMF characterisation.
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3

Optical Characterization of 3D Metasurface Microprints

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For the optical characterisation of the microprints of the 3D-integrated metasurfaces, we used an optical microscope (Carl-Zeiss AXIO-10) with ×5 (0.13NA), ×10 (0.25NA), ×50 (0.75NA), and ×100 (0.85NA) objective lenses. The different magnifications of the devices at each process step were captured with an incoherent white-light source under transmission mode. The microprint display performance was optimised by modifying the exposure time and contrast of the CCD. Then, the fabricated integrated devices were characterised for hologram projection by the experimental setup shown in Fig. S7. The three laser diodes emitting at 450 nm (Thorlabs CPS450), 532 nm (Thorlabs CPS532), and 633 nm (Thorlabs CPS633S) were exploited to generate R, G, and B channels. The polarizers were used to manipulate the source power to achieve a suitable RGB component ratio due to the polarisation-independent property of the proposed metasurfaces. Then, two dichroic lenses (DMLP567T and DMLP490T) were used to combine the three lasers. The hologram images were projected on a screen and captured by an SLR camera. To characterise the diffraction efficiency for each wavelength, we put an optical power metre in position 1 and position 2 as Fig. S7b shows to measure the transmission power and the diffraction power of the hologram image.
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4

Characterization of CsPbBr3 Perovskite Films

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The absorption and photoluminescence spectrum of CsPbBr3 films were, respectively, measured by UV–Visible absorption spectrometer (PERKIN-ELMER Lambda750) and fluorescence spectrometer (Edinburgh FS-5 UK); the fluorescence lifetime of CsPbBr3 films at excitation wavelength of 365 nm was measured by time-resolved fluorescence spectrophotometer (Edinburgh Photonics Mini-Tau). The performance of the photodetectors was characterized by a customized testing platform and all the tests were carried out in ambient condition. We use an arbitrary waveform generator (TeKtronix AFG3251C) as a voltage source to control the LED lamp (CREE XPE2) and laser lamp (Thorlabs CPS532). The light power density is calibrated by power meter (Newport 843-R). The voltage ampere characteristic curve of the photodetectors was tested with semiconductor parameter instrument (Keithley 4200). The response time and stability of the photodetector were tested with oscilloscope (KEYSIGHT DSOX1202A). A data acquisition system (multifunctional I/O device NI PCI-6259 and junction box NI SCB-68A) was used to measure the photocurrent value of the photodetector array in multi-channel real-time.
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5

Fluorescent Bead Dynamics Imaging

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In MMB (Figure 1b), a 3.5 mm diameter beam from a 532 nm laser diode module (CPS532, Thorlabs, Newton, NJ, USA), working at 0.25 mW, is focused to a 150 µm spot size by a pair of plano-convex lenses (ThorLabs, 200 and 50 mm focal lengths) and an objective lens (Newport, M-10X, 0.25 NA). The beam, diverted by a dichroic mirror (BrightLine Di02-R532, Semrock, Rochester, NY, USA) to the objective lens, exits the objective lens and is focused on a rectangular borosilicate sample cell (W2540, Vitrocom, Mountain Lakes, NJ, USA) containing the fluorescently tagged beads. The modulation frequency of the electromagnets is set to 1 Hz. The emitted fluorescence is collected using the same objective lens, passed through the dichroic mirror and two emission filters (FF03-575/25, Semrock, Rochester, NY, USA), and detected by a digital camera (GS3-U3-23S6M-C, FLIR, Wilsonville, OR, USA). A sequence of 600 images is acquired over a period of 12 s. The mean grey value (MGV) from the laser beam area of each image is calculated, and the peak-to-peak MGV differences over time are measured and averaged.
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6

Optical Tracking Microscope Design

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The tracking microscope was built predominantly using optics and an optical cage system from Thorlabs. The basic optical path consists of a 4x NA 0.1 Olympus PLAN objective (Thorlabs #RMS4X) with a 150 mm tube lens (Thorlabs #AC254-150-A-ML), yielding a magnification of 3.33X. The image is collected on a monochrome Chameleon3 camera (FLIR, #CM3-U3-13Y3M), with a 1/2" sensor format and 1280 × 1021 pixels (4.8 μm/px at sensor plane, 1.44 μm/px at object plane). This yields a field of view of 1.84 × 1.47 mm. We illuminated the sample with a 780 nm, 18 mW IR LED (Thorlabs #LED780E) in a transillumination configuration. The LED was diffused using a ground glass diffuser (Thorlabs #DG10-120), and collimated onto the sample using a f=16 mm aspheric condenser lens (Thorlabs #ACL25416U) placed 16 mm from the diffuser surface.
The microscope path also included a 4.5 mW, 532 nm laser diode module (Thorlabs #CPS532) for optogenetic stimulation. The laser beam (3.5 mm diameter) was combined with the main optical path using a 550 nm dichroic (Thorlabs #DMLP550R), then focused on the back focal point of the objective using a f = 100 mm planoconvex lens (Thorlabs #LA1509-A-ML). This illuminated a circular region roughly 1.6 mm in the sample plane, with a calculated average intensity of 150 μW/mm2.
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7

Spatially Modulated Laser Illumination for Multimode Fiber Characterization

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The experimental setup is illustrated in Fig. 2. The light source was a collimated diode-pumped solid-state laser module (532 nm, 4.5 mW, CPS532, Thorlabs, New Jersey, USA). After beam expansion through two achromatic lenses (AC254-030-A-ML; AC254-075-A-ML, Thorlabs, New Jersey, USA), the laser light was spatially modulated using a DMD (DLP7000, Texas Instruments, Texas, USA) and then projected onto the proximal facet of a MMF (105 μm, 0.22 NA, 1 m, M43L01, Thorlabs, New Jersey, USA) via a tube lens (AC254-050-A-ML, Thorlabs, New Jersey, USA) and an objective (20×, 0.4 NA, RMS20X, Thorlabs, New Jersey, USA). The light illuminated area on the DMD included 32 × 32 independent input modes, with each 2 × 2 micromirrors grouped as one mode. An objective (20×, 0.4 NA, RMS20X, Thorlabs, New Jersey, USA) and a tube lens (AC254-0100-A-ML, Thorlabs, New Jersey, USA) were used to magnify the output light beam before it was captured by a complementary metal-oxide-semiconductor (CMOS) camera (C11440-22CU01, Hamamatsu Photonics, Shizuoka, Japan) with a frame rate of 200 frames per second (fps) for MMF characterisation.
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8

Upconversion Luminescence Magnetic Sensing

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A 532 nm laser (maximum power, 4.5 mW; CPS532, Thorlabs) was used as the excitation source, and the laser beam was chopped and filtered with a 532 nm bandpass filter. A silicon detector (818-UV, Newport) measured upconverted emission intensity together with a lock-in amplifier (SR830, Stanford Research Systems). A 500 nm shortpass filter and a 532 nm notchpass filter were placed in front of the detector to collect only the upconverted emission. An electromagnet was switched between positive and zero magnetic fields every 20 s, and the PL change was estimated from four cycles. The magnetic field was recorded with a gaussmeter (HMMT-6J04-VF, Lakeshore). The four cycles of measurement were repeated for different magnetic fields to obtain Fig. 4a. The measurement was swept from a high field to a low field, and the data in Fig. 4a are an average of five independent sweeps. For NU-901:PtOEP, the PL change was estimated from three cycles, and Fig. 4b is an average of seven independent sweeps. The upconversion emission of ligand:PtOEP is weak, so an intense laser (4 W; Verdi G18, Coherent) was used. To avoid the photodegradation of the ligand, the PL change was estimated from two cycles and Fig. 4c is an average of four independent sweeps.
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