Amicon ultra 0.5 10 k
The Amicon Ultra-0.5 10 k is a centrifugal filter device designed for the concentration and purification of macromolecules. It utilizes a regenerated cellulose membrane with a molecular weight cut-off of 10 kDa to facilitate the separation and recovery of target molecules from complex samples.
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5 protocols using amicon ultra 0.5 10 k
Fluorescently Labeled Pol II Phospho-Specific Fabs
Optimized Expression and Purification of FP Variants
FP variants, cloned into the pQE30 vector (Qiagen) with a 6His tag at the N-terminus, were expressed in E. coli XL1 Blue strain (Evrogen). The proteins were isolated by ultrasonic cell lysis, purified using TALON metal-affinity resin (Clontech), eluted from the adsorbing material by treatment with 100 mM imidazole (pH 8) and then desalted by ultrafiltration with Amicon® Ultra-0.5 10 K (Merck) filters. All isolation and chromatography procedures were performed in PBS buffer pH 7.4 (Gibco). The same solution was further used for the specimen storage and spectroscopy measurements.
Exosome Characterization and Labeling for DEP
Antibody-MtsA Binding Kinetics
Purification and Characterization of EGFP Variants
The time-resolved fluorescence spectroscopy of the purified protein was performed using a set of pH buffers in a pH range of 5.5–10.0. Each buffer contained 130 mM potassium gluconate, 20 mM sodium gluconate, 0.5 mM MgCl2, 0.2 mM EGTA and 30 mM Tris (pH 8.0–10.0), HEPES (pH 6.8–7.8) or MES (pH 5.5–6.6). Each specimen contained 5 μg/mL of EGFP-Y145L/S205V, dissolved in pH buffer at a ratio of 1:20 v/v.
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