1 2 distearoyl sn glycero 3 phosphoethanolamine n methoxy polyethylene glycol 2000 dspe mpeg2000

Manufactured by Xi’an Ruixi

Sourced in China, United States

DSPE-mPEG2000 is a phospholipid conjugate consisting of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE) covalently linked to methoxy-polyethylene glycol (mPEG) with an average molecular weight of 2000 Da. It is a common component used in the formulation of liposomes and other lipid-based drug delivery systems.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions) Opti mem medium, by Thermo Fisher Scientific (2 mentions) Trizol, by Thermo Fisher Scientific (2 mentions) Fm4 64 dye, by Thermo Fisher Scientific (2 mentions) 5 cyanine 5 cy5 labeled asos, by Tsingke (2 mentions) Reversed enzyme, by Takara Bio (2 mentions) Syber green enzyme, by Takara Bio (2 mentions) Mueller hinton broth, by Beijing Land Bridge Technology (2 mentions) Lysogeny broth, by Beijing Land Bridge Technology (2 mentions) N hydroxysuccinimide nhs, by Merck Group (1 mentions) Fm4 64, by Thermo Fisher Scientific (1 mentions) Trypsin, by Beyotime (1 mentions) Docetaxel, by Merck Group (1 mentions) Ir780, by Merck Group (1 mentions) Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions) Perfluoropentane pfp, by Apollo Scientific (1 mentions)

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DSPE-mPEG2000 (4 citations)

5 protocols using 1 2 distearoyl sn glycero 3 phosphoethanolamine n methoxy polyethylene glycol 2000 dspe mpeg2000

Aptamer-Functionalized Nanomedicine Synthesis

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1,2-Distearoyl-sn-glycero-3-phosphocholine (DSPC), cholesterol, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-mPEG2000) and DSPE-mPEG2000-COOH were purchased from Xi'an Ruixi Biological Technology Co., Ltd (Xi'an, China). 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl), poly(ethylene glycol) bis-(carboxymethyl) ether 600 and N-hydroxysuccinimide (NHS) were obtained from Sigma-Aldrich. Methanol, chloroform, PTX, isopropanol and ethyl alcohol were obtained from Shanghai Aladdin Biochemical Technology Co., Ltd (Shanghai, China). AS1411 aptamer was ordered from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China) as the following sequence: NH₂-AS1411: 5′-NH₂-C6-GGTGGTGGTGGTTGTGGTGGTGGTGG-3′.

Li J., Li Q., He M., Ding F., Cai L., Zhao M., Dong L., Wang Q, & Xu K. (2019). AS1411 aptamer-modified theranostic liposomes co-encapsulating manganese oxide nano-contrast agent and paclitaxel for MRI and therapy of cancer. RSC Advances, 9(60), 34837-34846.

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Synthesis and Characterization of DPP7 and DPP8

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Dendritic
polypeptides 7 (DPP7) and DPP8 (Table S1) were synthesized by China Peptides Co., Ltd. (Suzhou, China). Phosphorothioate-modified
ASOs, 5′-fluorescein amidites (FAM)-labeled ASOs, and primers
were synthesized by Sangon Biotech (Shanghai, China). 1,2-Distearoyl-sn-glycero-3-phosphoethanol-amine-N-methoxy
(poly(ethylene glycol))-2000 (DSPE-mPEG2000) was purchased from Xi’an
ruixi Biological Technology Co., Ltd. Lipofectamine 2000 was obtained
from Life Technologies (Invitrogen, CA). Bacterial strains Staphylococcus aureus (S. aureus ATCC29213) and methicillin-resistant S. aureus (MRSA) (USA300 LAC), mouse brain capillary endothelial cell line
(Bend.3) and human nonsmall cell lung cancer cell line (A549) were
stored in our laboratory. FM4–64 was purchased from Life Technologies
(Invitrogen, CA).

Li Y., Hu Y., Kamal Z., Chen Y., Xue X., Yao S., Zhao H., Jia M., Li Y., Wang Z., Li M, & Chen Z. (2024). Optimization of Dendritic Polypeptide Delivery System for Antisense Antibacterial Agents Targeting ftsZ. ACS Omega, 9(19), 20966-20975.

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Synthesis and Characterization of Peptide-Based Therapeutics

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DPPs (Scheme 1, Additional file 1: Table S1, S2 and Fig. S1–S3) were synthesized by ChinaPeptides Co., Ltd. (Suzhou, China). 2′-O-methyl (2′-OMe) modified antisense oligonucleotides (ASOs), 5′-fluorescein amidites (FAM)-labeled ASOs and primers were synthesized by Sangon Biotech (Shanghai, China) (Additional file 1: Fig. S3). 5′-Cyanine 5 (Cy5)-labeled ASOs were synthesized by Tsingke Biological Technology Co., Ltd (Xi’an, China). 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy(polyethylene glycol)-2000 (DSPE-mPEG2000) was purchased from Xi'an ruixi Biological Technology Co., Ltd. Trizol (Additional file 1: Fig. S3), FM4-64 dye, Lipofectamine 2000™ and Opti-MEM medium were purchased from Life Technologies (Invitrogen, CA, USA). Lysogeny broth and Mueller–Hinton (M–H) broth were purchased from Beijing Land Bridge Technology Co., Ltd (Beijing, China). Reversed enzyme and SYBER Green enzyme were purchased from Takara Bio Inc. (Kyoto, Japan).

Chen Z., Hu Y., Mao X., Nie D., Zhao H., Hou Z., Li M., Meng J., Luo X, & Xue X. (2022). Amphipathic dendritic poly-peptides carrier to deliver antisense oligonucleotides against multi-drug resistant bacteria in vitro and in vivo. Journal of Nanobiotechnology, 20, 180.

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Lipid-based Nanoparticle for Targeted Drug Delivery

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1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy (polyethylene glycol)-2000 (DSPE-mPEG2000) and 1,2-dipalmitoyl-sn-glycero-3-phospha-tidylcholine (DPPC) were purchased from Xi’an Ruixi Biological Technology (Xi’an, China). IR780, propidium iodide (PI), cholesterol, and docetaxel were purchased from Sigma–Aldrich (Saint Louis, USA).. Anti-PSMA antibodies were purchased from Abcam (Cambridge, UK). Cell Counting Kit-8 (CCK-8) was obtained from Dojindo (Kumamoto, Japan). 4′,6-Diamidino-2-phenylindole (DAPI), 1,1-dioctadecyl-3,3,3,3-tetramethyl indocarbocyanine perchlorate (DiI), and 0.25% trypsin were purchased from Beyotime Biotechnology (Shanghai, China). Chloroform was obtained from Chuandong Chemical Co. Ltd. (Chongqing, China). Perfluoropentane (PFP) was obtained from Apollo Scientific Ltd. (Cheshire, UK).

Dai J., Wu Y., Chen Z., Xiao L., Zhang W, & Cao Y. (2023). Sonosensitive Phase-Changeable Nanoparticle Mediated Enhanced Chemotherapy in Prostate Cancer by Low-Intensity Focused Ultrasound. International Journal of Molecular Sciences, 24(1), 825.

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Synthesis and Labeling of Peptides

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DPPs (Scheme 1, Additional le 1: Table S1-S2 and Figure S1-S3) were synthesized by ChinaPeptides Co., Ltd. (Suzhou, China). 2′-O-methyl (2′-OMe) modi ed antisense oligonucleotides (ASOs), 5′-uorescein amidites (FAM)-labeled ASOs and primers were synthesized by Sangon Biotech (Shanghai, China) (Additional le 1: Figure S3). 5′-Cyanine 5 (Cy5)-labeled ASOs were synthesized by Tsingke Biological Technology Co., Ltd (Xi'an, China). 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-Nmethoxy(polyethylene glycol)-2000 (DSPE-mPEG2000) was purchased from Xi'an ruixi Biological Technology Co., Ltd. Trizol (Additional le 1: Figure S3), FM4-64 dye, Lipofectamine 2000 TM and Opti-MEM medium were purchased from Life Technologies (Invitrogen, CA, USA). Lysogeny broth and Mueller-Hinton (M-H) broth were purchased from Beijing Land Bridge Technology Co., Ltd (Beijing, China). Reversed enzyme and SYBER Green enzyme were purchased from Takara Bio Inc. (Kyoto, Japan).

Zhou C., Hu Y., Mao X., Nie D., Zhao H., Hou Z., Li M., Meng J., Luo X., & Xue X. (2021). Amphipathic Dendritic Poly-peptides Carrier to Deliver Antisense Oligonucleotides Against Multi-drug Resistant Bacteria in Vitro and in Vivo.

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