Anti bcl xl 2762s

Sorafenib (Selleck Chemicals, Houston, TX, USA), auranofin (MCE, Houston, TX, USA), and Gefitinib (Selleck Chemicals, Houston, TX, USA) were dissolved in DMSO at a stock concentration of 10 mM, Erlotinib (Selleck Chemicals, Houston, TX, USA) was dissolved in DMSO at a stock concentration of 5 mM and stored in aliquots at −20 °C. MTS assay Kit (CellTiter 96 Aqueous One Solution reagent) was purchased from Promega Corporation (Madison, WI, USA). Annexin V-FITC/PI apoptosis Detection Kit was purchased from Keygen Company (Nanjing, China). Mito-SOX were purchased from Invitrogen (Carlsbad, CA, USA). Anti-TXNRD2 (ab16841), anti-Trx2 (ab185544), anti-MCL-1 (ab32087), and anti-TXNRD3 (ab134034) antibodies were purchased from Abcam (Cambridge, MA, USA); Anti-HSP60 (#4870S), anti-TXNRD1 (#15140s), anti-α-tubulin (#2144), anti-β-actin (#4970), anti-Bcl-2 (#3498s), and anti-Bcl-xL (#2762s) antibodies were from Cell Signaling Technologies (Danvers, MA, USA).