Anti mouse f4 80 clone a3 1

The following anti-mouse Abs were used: CD45 (clone 30-F11), CD11b (clone M1/70), Ly6C (clone HK1.4), Ly6G (clone 1A8), CX₃CR1 (clone SA011F11), CD64 (clone X54-5/7.1), MHCII (clone M4-114.15.2)—all purchased from BioLegend (San Diego, USA). Anti-mouse F4/80 (clone A3-1) was purchased from BIORAD. Anti MMP-14 was purified in-house from hybridoma cells of LEM-2/15 (33 (link)). The cells were incubated with Abs for 30 min in FACS buffer (dark, 4°C) and then washed once with FACS buffer. Cells were analyzed with BD FACSCanto™ II (BD Bioscience). Flow cytometry analysis was performed using FlowJo software (TreeStar, Ashland, OR, USA).

The following antibodies were used for flow cytometry analysis (dilutions are indicated): anti-mouse CD45 (clone 30-F11, 1:100), anti-mouse/human CD11b (clone M1/70, 1:300), anti-mouse Ly6C (clone HK1.4, 1:300), anti-mouse MHCII (clone M5/114.15.2, 1:200), anti-mouse CD64 (clone X54-5/7.1, 1:50), anti-mouse Ly6G (clone 1A8, 1:100), and anti-mouse Tim4 (clone RMT4-54, 1:50), which were purchased from BioLegend, San Diego, CA, USA. Anti-mouse F4/80 (clone A3-1, 1:50) was purchased from BIORAD. The staining for ROS was performed with 0.1 mM of 2,7-dichlorodihydrofluoresceindiacetate (Molecular Probes Invitrogen). Staining for apoptosis and necrosis markers with Annexin V and propidium iodide was performed with MEBCYTO-Apoptosis Kit (MBL International Corporation). Cells were analyzed with BD FACSCanto™ II (BD Bioscience). Flow cytometry analysis was performed using FlowJo software (TreeStar, Ashland, OR, USA).

The following anti-mouse antibodies were used (dilutions are indicated): CD45 (clone 30-F11, 1:100), CD11b (clone M1/70, 1:300), Ly6C (clone HK1.4, 1:300), CD115 (clone AFS98, 1:100), CD64 (clone X54-5/7.1, 1:50), Ly6G (clone 1A8, 1:100), and Tim4 (clone RMT4-54, 1:50), MHCII (clone M4-114.15.2, 1:200), CX₃CR1 (clone SA011F11, 1:100), CD14 (clone M5E2, 1:100), CD16 (clone 3G8, 1:100) all purchased from BioLegend, San Diego, CA, USA. Anti-mouse F4/80 (clone A3-1, 1:50) was purchased from BIORAD. Cells were analyzed with BD FACSCanto™ II (BD Bioscience). Flow cytometry analysis was performed using FlowJo software (TreeStar, Ashland, OR, United States).