Small animal pet ct scanner

All animal experiments were conducted under the approved Administrative Panel on Laboratory Animal Care protocol at Stanford University. Control, AAVR-KO and SELECTIV-KO-Alb mice (male and female, 8–10 weeks old, n = 3) were anesthetized with 3.0% isoflurane in oxygen and maintained under 1.5–2.0% isoflurane while scanning. ⁶⁴Cu-AAV9 (n = 9, 201 ± 10 kBq) was administered via tail vein injection to mice on a small animal PET/CT scanner (Siemens). Mice were scanned for 30 min at 21 h post-injection. After the final PET/CT scan at 21 h, mice were euthanized by Euthasol under deep isoflurane and perfused with DMEM and PBS solution. Blood, heart, lungs and liver were collected and radioactivity in each organ was measured with a gamma counter. The biodistribution of ⁶⁴Cu-AAV9 was quantified as %ID g⁻¹ after decay correction.

Each B16-OVA tumor-bearing mouse (n = 4/group) was intravenously injected with 3.7 MBq of ⁶⁴Cu-labeled CTLs isolated from mice immunized with or without OVA (OVA-CTLs or control-CTLs), and 10-min static PET scans were obtained at 4, 12, 24, and 36 h postinjection using a small-animal PET/CT scanner (Siemens Medical Solutions). The PET images were analyzed, and the region-of-interest-derived percentage injected dose per gram of tissue (%ID/g) was calculated. In a separate experiment, B16-OVA tumor-bearing mice (n = 4/group) were administered with PLGA or PLGA-FAKi (50 μg of FAKi equivalent) on day 0 via their tail veins. On day 5, the mice were intravenously injected with 3.7 MBq of ⁶⁴Cu-labeled OVA-CTLs, and 10-min static PET scans were obtained at 24 and 32 h postinjection.

PET/CT was performed at baseline and on days 3, 17, 31, and 59, following TBI (Figure 1C). For scan preparation, at each time point, food was withheld overnight to ensure that the radioactive tracer (¹⁸F-FDG) was fully enriched in the rat brain during scanning.
Positron emission computed tomography scanning procedure was as follows. The body weight of the rats was recorded to calculate the dose of ¹⁸F-FDG administered intravenously. A 40 min wait was necessary after the injection to ensure full absorption. Imaging acquisition was performed using a small-animal PET/CT scanner (Siemens Inc., Erlangen, Germany). An animal anesthetizing evaporator was used to anesthetize rats with halothane gas (dose of 5%). The rat was then placed in a prone position on the scanning bed. A dose of 1.5% halothane gas was used continuously. After attenuation correction, PET images (axial, coronal, and sagittal views) of the rats were reconstructed using the OSEM3D mode. The images were acquired using the following parameters: current, 500 μA; spherical tube voltage, 80 kV; and CT time, 492 s.