The largest database of trusted experimental protocols

3 fluorescently labeled ssdna oligonucleotides

Manufactured by Integrated DNA Technologies

3'-fluorescently labeled ssDNA oligonucleotides are single-stranded DNA molecules with a fluorescent label attached to the 3' end. They are used as probes or primers in various molecular biology applications.

Automatically generated - may contain errors

2 protocols using 3 fluorescently labeled ssdna oligonucleotides

1

Probing APOBEC3 DNA interactions

Check if the same lab product or an alternative is used in the 5 most similar protocols
The ssDNA oligonucleotides for co-crystallization with A3A and A3Bctd* were 5′-AAAAAAATCGGGAAA and 5′-TTTTCAT, respectively (Integrated DNA Technologies). The unbiased experimental approach for identifying the former sequence is described below, and the latter is based on the fact that 5′-TCA is the most commonly mutated APOBEC signature motif in cancer13 (link)–15 (link). 3′-fluorescently labeled ssDNA oligonucleotides for in vitro DNA deamination experiments were obtained from Integrated DNA Technologies or Midland Certified Reagent Company. The ssDNA oligonucleotide substrates used in Fig. 3e are 5′-AAAAAAAAATCGGGAAAAAAA-3′-FAM, 5′-AAAAAAAAA[dU]CGGGAAAAAAA-3′-FAM, 5′-AAAAAAAAA[5-F-dU]CGGGAAAAAAA-3′-FAM, and 5′-AAAAAAAAA[iSuper-dT]CGGGAAAAAAA-3′-FAM (labeled dT, dU, Super T, and 5FdU, respectively). The ssDNA oligonucleotide substrate used in Fig. 5a is 5′-ATTATTATTATTCAAATGGATTTATTTATTTATTTATTTATTT-3′-FAM, the same except (A, C, G, or T)CA as trinucleotide targets in Fig. 5b. 5-Nitroindole containing ssDNA substrates were purchased from Integrated DNA Technologies (Coralville, IA) as desalted oligonucleotides and characterized by LC-MS on an Agilent 1100 series HPLC instrument equipped an Agilent MSD SL ion trap mass spectrometer. A full list of ssDNA oligonucleotides used in site-directed mutation is available upon request.
+ Open protocol
+ Expand
2

Probing APOBEC3 DNA interactions

Check if the same lab product or an alternative is used in the 5 most similar protocols
The ssDNA oligonucleotides for co-crystallization with A3A and A3Bctd* were 5′-AAAAAAATCGGGAAA and 5′-TTTTCAT, respectively (Integrated DNA Technologies). The unbiased experimental approach for identifying the former sequence is described below, and the latter is based on the fact that 5′-TCA is the most commonly mutated APOBEC signature motif in cancer13 (link)–15 (link). 3′-fluorescently labeled ssDNA oligonucleotides for in vitro DNA deamination experiments were obtained from Integrated DNA Technologies or Midland Certified Reagent Company. The ssDNA oligonucleotide substrates used in Fig. 3e are 5′-AAAAAAAAATCGGGAAAAAAA-3′-FAM, 5′-AAAAAAAAA[dU]CGGGAAAAAAA-3′-FAM, 5′-AAAAAAAAA[5-F-dU]CGGGAAAAAAA-3′-FAM, and 5′-AAAAAAAAA[iSuper-dT]CGGGAAAAAAA-3′-FAM (labeled dT, dU, Super T, and 5FdU, respectively). The ssDNA oligonucleotide substrate used in Fig. 5a is 5′-ATTATTATTATTCAAATGGATTTATTTATTTATTTATTTATTT-3′-FAM, the same except (A, C, G, or T)CA as trinucleotide targets in Fig. 5b. 5-Nitroindole containing ssDNA substrates were purchased from Integrated DNA Technologies (Coralville, IA) as desalted oligonucleotides and characterized by LC-MS on an Agilent 1100 series HPLC instrument equipped an Agilent MSD SL ion trap mass spectrometer. A full list of ssDNA oligonucleotides used in site-directed mutation is available upon request.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!