Cd335 nkp46 efluor450

Manufactured by Thermo Fisher Scientific

The CD335 (NKp46) eFluor450 is a fluorescently labeled antibody that binds to the CD335 (NKp46) antigen. It can be used for the identification and analysis of natural killer cells in flow cytometry applications.

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Lab products found in correlation

Facscanto, by BD (1 mentions) Ccr7 pe, by BD (1 mentions) Cd3 apc, by BioLegend (1 mentions) Cd45ra fitc, by Miltenyi Biotec (1 mentions) Cd16 pe cy7, by Thermo Fisher Scientific (1 mentions) Anti human cd19 fitc, by Miltenyi Biotec (1 mentions) Cd8 apc, by Miltenyi Biotec (1 mentions) Cd19 apc efluor780, by Thermo Fisher Scientific (1 mentions) Cd3 fitc, by Thermo Fisher Scientific (1 mentions) Cd4 percp, by Thermo Fisher Scientific (1 mentions)

2 protocols using cd335 nkp46 efluor450

Phenotypic Analysis of Lymphocyte Subsets in Ovarian Cancer

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Peripheral lymphocytes (OC patients: n = 27; normal: n = 10) and ascites-associated lymphocyte subsets (n = 43) were characterized using the following antibodies for surface expression by flow cytometry (FACSCanto BD Bioscience): anti-human CD19 FITC, CD45RA FITC, CD8 APC (all Miltenyi Biotec), CD16 PE-Cy7 and CD335 (NKp46) eFluor450 (both eBiosciences), CD4 PE-Cy7 (Southern Biotech), CD3 APC (Biolegend), CCR7 PE (BD Biosciences), and NKG2D FITC (abcam). Corresponding isotype-matched controls were purchased from Miltenyi Biotec, BD Biosciences and eBioscience. TAMs in ascites were analyzed for surface expression of CD14, CD163, CD206 and intracellular expression of CD68 by flow cytometry, as described previously.³⁶ (link)

Vyas M., Reinartz S., Hoffmann N., Reiners K.S., Lieber S., Jansen J.M., Wagner U., Müller R, & von Strandmann E.P. (2017). Soluble NKG2D ligands in the ovarian cancer microenvironment are associated with an adverse clinical outcome and decreased memory effector T cells independent of NKG2D downregulation. Oncoimmunology, 6(9), e1339854.

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Splenocyte Lymphocyte Subpopulation Analysis

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Freshly isolated splenocytes (1 × 10⁶ cells) were stained for characterization of lymphocytes sub-populations, i.e. T cells (CD3 + CD4+ and CD3 + CD8+), B cells (CD3-CD19+) and NK cells (CD3-CD335+), using fluorochrome-conjugated antibodies CD3 FITC, CD4 PerCP, CD8a (Ly-2) PE, CD19 APCeFluor780 and CD335 (NKp46) eFluor450 (eBioscience). Pooled samples (per group) were used as negative and FMO (Fluorescence Minus One) controls in addition to Isotype controls.

Tobias J., Jasinska J., Baier K., Kundi M., Ede N., Zielinski C, & Wiedermann U. (2017). Enhanced and long term immunogenicity of a Her-2/neu multi-epitope vaccine conjugated to the carrier CRM197 in conjunction with the adjuvant Montanide. BMC Cancer, 17, 118.

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