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T mtorc1

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T-mTORC1 is a molecular probe that targets and detects the mammalian target of rapamycin complex 1 (mTORC1). mTORC1 is a serine/threonine protein kinase complex that plays a central role in regulating cell growth, proliferation, and metabolism in response to various cellular cues.

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Lab products found in correlation

P akt, by Cell Signaling Technology (2 mentions) P mtorc1, by Cell Signaling Technology (2 mentions) T akt, by Cell Signaling Technology (2 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Collagenase, by Thermo Fisher Scientific (1 mentions) Glyceraldehyde 3 phosphate dehydrogenase gapdh, by Cell Signaling Technology (1 mentions) T p70s6 kinase, by Cell Signaling Technology (1 mentions) P jnk t183 y185, by Cell Signaling Technology (1 mentions) P tak1, by Cell Signaling Technology (1 mentions) α actinin, by Cell Signaling Technology (1 mentions) Ang 2, by Enzo Life Sciences (1 mentions) P p70 s6 kinase thr389, by Cell Signaling Technology (1 mentions) T tak1, by Cell Signaling Technology (1 mentions) T erk, by Cell Signaling Technology (1 mentions) T jnk, by Cell Signaling Technology (1 mentions) P erk, by Cell Signaling Technology (1 mentions) α tubulin, by Merck Group (1 mentions) P erk1 2, by Cell Signaling Technology (1 mentions) Vimentin, by Abcam (1 mentions) P perk, by Cell Signaling Technology (1 mentions)

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MTORC1 (15 citations)

2 protocols using t mtorc1

1

Angiotensin II-Induced Signaling Pathway

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Ang II was purchased from ENZO (ALX-151-039-M025); collagenase and trypsin were purchased from Gibco (Grand Island, NY, United States); the BCA protein assay kit was bought from Pierce (Rockford, United States); and 2,7-dichlorofluorescindiacetate (DCFH-DA) was obtained from the Bioengineering Institute (Nanjing, China). The following primary antibodies were obtained from Cell Signaling Technology (CST, United States): glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (#2118), p-mTORC1 (#2971), T-mTORC1 (#2983), α-actinin (#69758S), P-p70 S6 kinase (Thr389) (#9234P), T-p70 S6 kinase (#2708), P-JNK (T183/Y185) (#4668P),T-JNK (#9258), P-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (#4370P), T-ERK (#4695), P-p38 (#4511P), p38 MAPK (#9212P), T-TAK1 (#5206), P-TAK1 (#4508), T-AKT (#4691), P-AKT (#4060), acetyl-CoA carboxylase antibody (#3676), and P-acetyl-CoA carboxylase antibody (#3661S). ABCAM provided the following primary antibodies: Anti-AMPKα2 (ab3760), p-AMPKα2 (S491, ab109402), anti-SOD1 (ab16831), anti-SOD2 (ab38155), Nrf2 (ab15323), 4-hydroxynonenal (ab46545), sarcomeric α-actinin (ab68167), heme oxygenase1 (ab-13243), and NOX2/gp91phox (ab129068). The Sesn2 antibody was acquired from Proteintech (no. 10795-1-AP). Antibodies were used at 1:1,000 dilutions for Western blotting. The secondary antibodies were obtained from LI-COR Biosciences (Lincoln, United States).
Zhang N., Liao H.H., Feng H., Mou S.Q., Li W.J., Aiyasiding X., Lin Z., Ding W., Zhou Z.Y., Yan H., Chen S, & Tang Q.Z. (2021). Knockout of AMPKα2 Blocked the Protection of Sestrin2 Overexpression Against Cardiac Hypertrophy Induced by Pressure Overload. Frontiers in Pharmacology, 12, 716884.
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2

Antibody Panel for Cellular Signaling

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The following antibodies were used for IHC and Western immunoblotting: ATF4 (catalog 10835-1-AP) and SKP2 (catalog 15010-1-AP) from Proteintech; CHOP (catalog 2895), p-YAP (catalog 13008, S127), YAP (catalog 14074), p27 (catalog 3686), MYC (catalog 9402), p-RPS6 (catalog 4858, S235/236), t-RPS6 (catalog 2217), p-mTORC1 (catalog 5536, S2448), t-mTORC1 (catalog 2983), p-ERK1/2 (catalog 9101, T202/Y204), t-eIF2α (catalog 2103), p-AKT (catalog 9271, S473), t-AKT (catalog 4691), EIF4FBP1 (catalog 2855, T37/46), K48-Ub (catalog 8081), p-PERK (catalog 3179, T980), and PERK (catalog 3192) from Cell Signaling Technology; CYR61 (catalog E-AB-14920), CTGF (E-AB-12339), and aquaporin 2 (E-AB-30540) from Elabscience Biotechnology; CDK1 (catalog SC-54) and t-ERK1/2 (catalog SC-94) from Santa Cruz Biotechnology; α-tubulin (catalog T5168) and K63-Ub (catalog 05-1308) from MilliporeSigma; PCNA (catalog MS-106-P1ABX and, p-LATS1/2 (catalog PA5-64591, S809/S872) from Thermo Fisher Scientific; Oct4 (catalog NB100-2379) from Novus Biologicals; Sox2 (catalog ab97959), vimentin (catalog ab92547), Ki-67 (clone SP6), p-eIF2α (catalog ab32157, S51), BIM (catalog ab32158), and TAZ (catalog ab224239) from Abcam; GRP78 (catalog A0241) from StressMarq Biosciences; and nestin 1 (monoclonal rat-401s) from DSHBU Iowa.
Panda D.K., Bai X., Zhang Y., Stylianesis N.A., Koromilas A.E., Lipman M.L, & Karaplis A.C. (2022). SCF-SKP2 E3 ubiquitin ligase links mTORC1/ER stress/ISR with YAP activation in murine renal cystogenesis. The Journal of Clinical Investigation, 132(24), e153943.
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