Antibodies against dj 1

A total of 40 male Sprague Dawley rats (weight, 250–300 g; age, 9–12 weeks) were purchased from Wuhan University, Animal Biosafety Level 3 Laboratory (Wuhan, China). All rats were housed under standard laboratory conditions at 22–24°C, a relative humidity of 50±15%, and a 12 h light/dark cycle. All animals had free access to standard rat chow and water. The experimental protocol of the present study was reviewed and approved by the Animal Care and Use Committee of Wuhan University (Wuhan, China), and was in accordance with the Guide for the Care and Use of Laboratory Animals by the National Institutes of Health. Streptozotocin (STZ) was purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). Antibodies against DJ-1 were purchased from Cell Signaling Technology, Inc. (cat no. 5933; Beverly, MA, USA). Antibodies to Nrf2 were purchased from Santa Cruz Biotechnology, Inc. (cat no. sc722; Dallas, TX, USA). GAPDH and Lamin B were purchased from Cell Signaling Technology, Inc. (cat nos. 5174 and 13435), and secondary antibodies were purchased from Santa Cruz Biotechnology, Inc. (cat no. sc2357). All other chemicals were obtained from commercial sources and were of the highest grade available.