All sample extracts were analyzed using an Agilent 8890A gas chromatography column equipped with a 5977B mass spectrometer (Agilent, Shanghai, China). The gas chromatographic column was an Agilent 19091S-433UI column (30 m × 0.25 mm i.d., 0.25 μm film thickness). The injection volume was 1.0 µL. The settings used for the temperature program were as follows: a starting temperature of 60 °C, increase to 160 °C at 8 °C·min−1, and then increase to 220 °C at 10 °C·min−1. The total GC runtime was 18.5 min. The carrier gas was nitrogen, which flowed at a rate of 1.0 mL·min−1. The quadrupole was at 150 °C, and the ion source was at 200 °C. The solvent delay time was 5.0 min. The electron impact ion energy was 70 eV. The mass scan range was m/z 50–500.
19091s 433ui
Manufactured by Agilent Technologies
Sourced in United States
The 19091S-433UI is a lab equipment product from Agilent Technologies. It is designed to perform a core function for laboratory use. However, a detailed and unbiased description of its features and capabilities cannot be provided while maintaining the requested level of conciseness and objectivity.
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Lab products found in correlation
5977b mass spectrometer, by Agilent Technologies (1 mentions)
Ms 5977, by Agilent Technologies (1 mentions)
Gc 8890, by Agilent Technologies (1 mentions)
Hp 5ms ui column, by Agilent Technologies (1 mentions)
Agilent 5977a gc msd system, by Agilent Technologies (1 mentions)
Hp 5ms ultra inert column, by Agilent Technologies (1 mentions)
7 protocols using 19091s 433ui
1
GC-MS Analysis of Organic Compounds
2
GC-MS Analysis of Enzyme Activation Reaction
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The product of the in vitro enzyme activation reaction was detected using a Gas Chromatography-Mass Spectrometry spectrometer (Agilent Technologies, Santa Clara, CA, USA) on a column of Agilent 19091S-433UI (30.0 m × 250 μm × 0.25 μm) with an initial column temperature of 50 °C. The procedure lasts for 2 min, and then heated to 250 °C at 6 °C/min for 3 min. The scanning time started at 4.0 min and ended at 33.16665 min. The temperature of the vaporization chamber was 230 °C, and the injection volume was 1.0 μL. The carrier gas was pure helium with a flow rate of 1.0 mL/min and a pre-column pressure of 7.6522 psi. The mass spectrometry conditions were as follows: EI ion source, ion source temperature of 230 °C, the quadrupole temperature of 150 °C, the electron energy of 70 eV, an emission current of 34.6 μA, a multiplier voltage of 962 V, an interface temperature of 250 °C, and a mass range of 50–350 amu.
3
GC-MS Analysis of Essential Oils and VOCs
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The EOs and VOCs were analyzed using an Agilent GC8890 plus MS5977 instrument (Agilent, Shanghai, China). The chromatographic column was an Agilent 19091S-433UI (30 m × 250 μm × 0.25 μm, -60°C-325°C). The injection volume was 1 μL. The temperature program employed for EO detection had the following settings: an initial temperature of 50°C, an increase to 120°C at 5°C/min, an increase to 180°C at 4°C/min, and then an increase to 300°C at 10°C/min. The ion source and quadrupole were 200 and 150°C, respectively. The temperature program employed for VOC detection had the following settings: an initial temperature of 40°C, an increase to 140°C at 10°C/min, an increase to 200°C at 5°C/min, and then an increase to 300°C at 8°C/min. The ion source and quadrupole were 230 and 150°C, respectively.
4
GC-MS Analysis of Boswellia carterii Oil
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Boswellia carterii essential oil extract was analyzed with gas chromatography (GC) (7890B), equipped with Agilent 19091S-433 UI, HP-5MS UI column (30 m × 0.25 mm internal diameter and 0.25 μm film thickness), and mass spectrometer detector (MS) (5977B). Helium was chosen to be the carrier gas, flowing with a rate of 1.0 mL min−1 and at a split ratio of 30 : 1. A volume of 1 μL of Boswellia carterii essential oil extract was injected, and the temperature program was set to 40 °C for 1 min then it raised to 150 °C with a rate of 4 °C min−1 and held for 6 min. Then, temperature was raised again to reach 210 °C with a rising rate of 4 °C min−1 and held for another 1 min. Finally, temperatures of 280 °C and 220 °C were set for the injector and detector, respectively. For MS, 70 eV was set for electron ionization (EI) with m/z ranging between 50 and 450 and 6 min for solvent delay. And finally, the sample was run for 50.5 min to identify the extracted oil constituents. Wiley and NIST Mass Spectral Library data was used to identify the sample's constituents via comparing the obtained spectrum fragmentation pattern.33,34 (link)
5
Quantitative GC-MS Analysis of Plant Wax
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Buds were cut with a blade, and the wax on them was extracted with chloroform for 3 min (ten buds as a biological replication with three procedural replications). Aliquots of 2 μL of tetracosane were added to each sample bud as an internal standard. Samples dissolved in chloroform were air-dried in the fume hood. Aliquots of 40 μL of pyridine (Sigma, P57506, Kawasaki, Japan) and 40 μL of bis (trimethylsilyl) trifluoroacetamide (Solarbio, B8810, Beijing, China) were added into the samples, followed by incubation at 70 ℃ for 40 min for derivatization. This derivatization mixture was dried under a gentle nitrogen stream. Samples were then re-suspended in 1 mL of chloroform, followed by filtration through a 0.45 μm membrane.
Quantitative analysis of wax content was conducted on a GC-MS (Agilent 7890B-7000D, Santa Clara, CA, USA). The capillary column (Agilent 19091S-433UI) was used with helium as carrier gas (1.2 mL/min). The oven was conditioned at 50 °C for 1 min. The temperature was then increased to 170 °C at a rate of 20 °C/min for 2 min, followed by an increase to 300 °C at a rate of 5 °C/min for 15 min. The data thus acquired were analyzed by GC-MS solution software. Peaks were identified with the help of a NIST library and wax standards.
Quantitative analysis of wax content was conducted on a GC-MS (Agilent 7890B-7000D, Santa Clara, CA, USA). The capillary column (Agilent 19091S-433UI) was used with helium as carrier gas (1.2 mL/min). The oven was conditioned at 50 °C for 1 min. The temperature was then increased to 170 °C at a rate of 20 °C/min for 2 min, followed by an increase to 300 °C at a rate of 5 °C/min for 15 min. The data thus acquired were analyzed by GC-MS solution software. Peaks were identified with the help of a NIST library and wax standards.
6
Purity Characterization by GC-MS and NMR
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Purity was inspected by GC–MS (43 (link)) and NMR. Samples were dissolved in ethyl acetate and subjected to GC–MS on an Agilent 5977A GC/MSD system—7890B Agilent GC system with an ultrainert GC column (19091S-433UI) followed by an Agilent 5977A MS instrument. Helium was used as the carrier gas at a constant flow of 0.7 mL/min, and 1:50 split samples were injected. Injection port and MS source temperature were held constant at 230 °C, and the MS quad temperature was held constant at 150 °C. The column temperature gradient was as follows: 70 °C for 0.5 min, 25 °C/min to 150 °C, 15 °C/min to 200 °C, and 25 °C/min to 300 °C and held at the upper temperature for 1 min. Results were analyzed on Mass Hunter software, the mass spectrum was compared to Wiley/NIST 2014 library (SI Appendix, Fig. S3A–C ). For NMR analysis, samples were dissolved in deuterated chloroform (CDCl3) in NMR tubes and were analyzed using an AVANCE III-400 device (Bruker) in 1H, 13C, and DEPT NMR modes. Resulting spectra were compared to National Institute of Advanced Industrial Science and Technology (AIST) spectral database. (SI Appendix, Fig. S3 D and E )
7
GC-MS Profiling of TBDMS and MeOX-TMS Derivatized Samples
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The GC-MS measurements were performed on an Agilent 7890B GC, electron impact ionisation (70 eV) equipped with an Agilent HP 5-ms ultra-inert column (Agilent 19091S-433UI, 30m x 250µm x 0.25 µm dimensions) at the facility in BioX center at IIT Mandi. In GC-MS, 1 µl sample volume was taken for injection. For TBDMS derivatised amino acid hydrolysate, the initial oven temperature was constant at 120 o C for 5 min, then a 4 o C /min ramped to 270 o C, held for 3 min, then a 20 o C/min ramped to 320 o C and held for 1 min. The carrier gas (Helium) flow was maintained at 1.3 ml min -1 . The spectra were recorded with a scanning range of 30 to 600 mz -1 for a total run time of 49 mins. For MeOX-TMS derivatised samples, oven temperature was constant at 50 o C for 5 mins and then ramped to 200 o C with 10 o C /min and held for 10 min. Temperature was again ramped to 300 o C with 5 o C /min and held for 10 min. After that temperature was decreased to 70 o C with 100 o C /min. The carrier gas (Helium) flow was 1.3 ml min -1 . MassHunter (Agilent Technologies, USA) was used to control the data acquisition parameters (both GC separation and mass spectrometry) during all the sample runs.
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